Tumor necrosis factor superfamily member APRIL contributes to fibrotic scar formation after spinal cord injury

BACKGROUND: Fibrotic scar formation contributes to the axon growth-inhibitory environment that forms following spinal cord injury (SCI). We recently demonstrated that depletion of hematogenous macrophages led to a reduction in fibrotic scar formation and increased axon growth after SCI. These changes were associated with decreased TNFSF13 (a proliferation inducing ligand (APRIL)) expression, but the role of APRIL in fibrotic scar formation after SCI has not been directly investigated. Thus, the goal of this study was to determine the role of APRIL in fibrotic scar formation after SCI. METHODS: APRIL knockout and wild-type mice received contusive SCI and were assessed for inflammatory cytokine/chemokine expression, leukocyte infiltration, fibrotic scar formation, axon growth, and cell proliferation. RESULTS: Expression of APRIL and its receptor BCMA is increased following SCI, and genetic deletion of APRIL led to reduced fibrotic scar formation and increased axon growth. However, the fibrotic scar reduction in APRIL KO mice was not a result of changes in fibroblast or astrocyte proliferation. Rather, APRIL knockout mice displayed reduced TNFα and CCL2 expression and less macrophage and B cell infiltration at the injury site. CONCLUSIONS: Our data indicate that APRIL contributes to fibrotic scar formation after SCI by mediating the inflammatory response

Wave constraints for Titan's Jingpo Lacus and Kraken Mare from VIMS specular reflection lightcurves

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A new digital terrain model of the Huygens landing site on Saturn's largest moon, Titan

© 2020. The Authors. River valleys have been observed on Titan at all latitudes by the Cassini-Huygens mission. Just like water on Earth, liquid methane carves into the substrate to form a complex network of rivers, particularly stunning in the images acquired near the equator by the Huygens probe. To better understand the processes at work that form these landscapes, one needs an accurate digital terrain model (DTM) of this region. The first and to date the only existing DTM of the Huygens landing site was produced by the U.S. Geological Survey (USGS) from high-resolution images acquired by the DISR (Descent Imager/Spectral Radiometer) cameras on board the Huygens probe and using the SOCET SET photogrammetric software. However, this DTM displays inconsistencies, primarily due to nonoptimal viewing geometries and to the poor quality of the original data, unsuitable for photogrammetric reconstruction. We investigate a new approach, benefiting from a recent reprocessing of the DISR images correcting both the radiometric and geometric distortions. For the DTM reconstruction, we use MicMac, a photogrammetry software based on automatic open-source shape-from-motion algorithms. To overcome challenges such as data quality and image complexity (unusual geometric configuration), we developed a specific pipeline that we detailed and documented in this article. In particular, we take advantage of geomorphic considerations to assess ambiguity on the internal calibration and the global orientation of the stereo model. Besides the novelty in this approach, the resulting DTM obtained offers the best spatial sampling of Titan's surface available and a significant improvement over the previous results

Geomorphologic mapping of titan's polar terrains: Constraining surface processes and landscape evolution

We present a geomorphologic map of Titan's polar terrains. The map was generated from a combination of Cassini Synthetic Aperture Radar (SAR) and Imaging Science Subsystem imaging products, as well as altimetry, SARTopo and radargrammetry topographic datasets. In combining imagery with topographic data, our geomorphologic map reveals a stratigraphic sequence from which we infer process interactions between units. In mapping both polar regions with the same geomorphologic units, we conclude that processes that formed the terrains of the north polar region also acted to form the landscape we observe at the south. Uniform, SAR-dark plains are interpreted as sedimentary deposits, and are bounded by moderately dissected uplands. These plains contain the highest density of filled and empty lake depressions, and canyons. These units unconformably overlay a basement rock that outcrops as mountains and SAR-bright dissected terrains at various elevations across both poles. All these units are then superposed by surficial units that slope towards the seas, suggestive of subsequent overland transport of sediment. From estimates of the depths of the embedded empty depressions and canyons that drain into the seas, the SAR-dark plains must be >600 m thick in places, though the thickness may vary across the poles. At the lowest elevations of each polar region, there are large seas, which are currently liquid methane/ethane filled at the north and empty at the south. The large plains deposits and the surrounding hillslopes may represent remnant landforms that are a result of previously vast polar oceans, where larger liquid bodies may have allowed for a sustained accumulation of soluble and insoluble sediments, potentially forming layered sedimentary deposits. Coupled with vertical crustal movements, the resulting layers would be of varying solubilities and erosional resistances, allowing formation of the complex landscape that we observe today