28 research outputs found
Present state and future perspectives of using pluripotent stem cells in toxicology research
The use of novel drugs and chemicals requires reliable data on their potential toxic effects on humans. Current test systems are mainly based on animals or in vitro–cultured animal-derived cells and do not or not sufficiently mirror the situation in humans. Therefore, in vitro models based on human pluripotent stem cells (hPSCs) have become an attractive alternative. The article summarizes the characteristics of pluripotent stem cells, including embryonic carcinoma and embryonic germ cells, and discusses the potential of pluripotent stem cells for safety pharmacology and toxicology. Special attention is directed to the potential application of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) for the assessment of developmental toxicology as well as cardio- and hepatotoxicology. With respect to embryotoxicology, recent achievements of the embryonic stem cell test (EST) are described and current limitations as well as prospects of embryotoxicity studies using pluripotent stem cells are discussed. Furthermore, recent efforts to establish hPSC-based cell models for testing cardio- and hepatotoxicity are presented. In this context, methods for differentiation and selection of cardiac and hepatic cells from hPSCs are summarized, requirements and implications with respect to the use of these cells in safety pharmacology and toxicology are presented, and future challenges and perspectives of using hPSCs are discussed
Subject-based didactics oscillating between the demands of competence-based learning and inclusive pedagogy: unification or concealment of incompability?
Globalization of Stem Cell Science: An Examination of Current and Past Collaborative Research Networks
Science and engineering research has becoming an increasingly international phenomenon. Traditional bibliometric studies
have not captured the evolution of collaborative partnerships between countries, particularly in emerging technologies
such as stem cell science, in which an immense amount of investment has been made in the past decade. Analyzing over
2,800 articles from the top journals that include stem cell research in their publications, this study demonstrates the
globalization of stem cell science. From 2000 to 2010, international collaborations increased from 20.9% to 36% of all stem
cell publications analyzed. The United States remains the most prolific and the most dominant country in the field in terms
of publications in high impact journals. But Asian countries, particularly China are steadily gaining ground. Exhibiting the
largest relative growth, the percent of Chinese-authored stem cell papers grew more than ten-fold, while the percent of
Chinese-authored international papers increased over seven times from 2000 to 2010. And while the percent of total stem
cell publications exhibited modest growth for European countries, the percent of international publications increased more
substantially, particularly in the United Kingdom. Overall, the data indicated that traditional networks of collaboration extant
in 2000 still predominate in stem cell science. Although more nations are becoming involved in international collaborations
and undertaking stem cell research, many of these efforts, with the exception of those in certain Asian countries, have yet to
translate into publications in high impact journals