The highly reintegrative approach of electronic monitoring in the Netherlands

This contribution describes the way electronic monitoring (EM) is organized and implemented in the Netherlands. It will become clear that the situation in the Netherlands is characterized by, in particular, two features. The application of EM is highly interwoven with the Probation Service and its reintegrative objectives, a characteristic that dominates the organization and use of EM to a great extent. Paradoxically, EM is hardly used in the Netherlands as an autonomous (stand-alone) replacement for short prison sentences. The most straightforward explanation for this situation is that the Netherlands does not really need EM to replace prison capacity since its prison population already decreased drastically since 2005. A second explanation is that the intense involvement of the probation service in the enforcement of electronic monitoring has as a side-effect that these sanctions are not accepted as punitive sanctions, but fully framed into the rehabilitative perspective.Criminal Justice: Legitimacy, accountability, and effectivit

Decoupling nutrient signaling from growth rate causes aerobic glycolysis and deregulation of cell size and gene expression

To survive and proliferate, cells need to coordinate their metabolism, gene expression, and cell division. To understand this coordination and the consequences of its failure, we uncoupled biomass synthesis from nutrient signaling by growing, in chemostats, yeast auxotrophs for histidine, lysine, or uracil in excess of natural nutrients (i.e., sources of carbon, nitrogen, sulfur, and phosphorus), such that their growth rates (GRs) were regulated by the availability of their auxotrophic requirements. The physiological and transcriptional responses to GR changes of these cultures differed markedly from the respective responses of prototrophs whose growth-rate is regulated by the availability of natural nutrients. The data for all auxotrophs at all GRs recapitulated the features of aerobic glycolysis, fermentation despite high oxygen levels in the growth media. In addition, we discovered wide bimodal distributions of cell sizes, indicating a decoupling between the cell division cycle (CDC) and biomass production. The aerobic glycolysis was reflected in a general signature of anaerobic growth, including substantial reduction in the expression levels of mitochondrial and tricarboxylic acid genes. We also found that the magnitude of the transcriptional growth-rate response (GRR) in the auxotrophs is only 40–50% of the magnitude in prototrophs. Furthermore, the auxotrophic cultures express autophagy genes at substantially lower levels, which likely contributes to their lower viability. Our observations suggest that a GR signal, which is a function of the abundance of essential natural nutrients, regulates fermentation/respiration, the GRR, and the CDC.National Institutes of Health (U.S.) (GM046406)National Institute of General Medical Sciences (U.S.) (Center for Quantitative Biology (GM071508