74 research outputs found
Measuring hsCRPâAn Important Part of a Comprehensive Risk Profile or a Clinically Redundant Practice?
James McCormack and Michael Allan discuss issues and questions surrounding hsCRP measurements in patients
Making connections across silos: intimate partner violence, mental health, and substance use
Anaerobiosis revisited: growth of Saccharomyces cerevisiae under extremely low oxygen availability
The budding yeast Saccharomyces cerevisiae plays an important role in biotechnological applications, ranging from fuel ethanol to recombinant protein production. It is also a model organism for studies on cell physiology and genetic regulation. Its ability to grow under anaerobic conditions is of interest in many industrial applications. Unlike industrial bioreactors with their low surface area relative to volume, ensuring a complete anaerobic atmosphere during microbial cultivations in the laboratory is rather difficult. Tiny amounts of O2 that enter the system can vastly influence product yields and microbial physiology. A common procedure in the laboratory is to sparge the culture vessel with ultrapure N2 gas; together with the use of butyl rubber stoppers and norprene tubing, O2 diffusion into the system can be strongly minimized. With insights from some studies conducted in our laboratory, we explore the question âhow anaerobic is anaerobiosis?â. We briefly discuss the role of O2 in non-respiratory pathways in S. cerevisiae and provide a systematic survey of the attempts made thus far to cultivate yeast under anaerobic conditions. We conclude that very few data exist on the physiology of S. cerevisiae under anaerobiosis in the absence of the anaerobic growth factors ergosterol and unsaturated fatty acids. Anaerobicity should be treated as a relative condition since complete anaerobiosis is hardly achievable in the laboratory. Ideally, researchers should provide all the details of their anaerobic set-up, to ensure reproducibility of results among different laboratories.
A correction to this article is available online at http://eprints.whiterose.ac.uk/131930/
https://doi.org/10.1007/s00253-018-9036-
A critical overview of progress in studies of migration of dragonflies (Odonata: Anisoptera), with emphasis on North America
Increased rate of positive penicillin skin tests among patients with glioma: insights into the association between allergies and glioma risk
Evaluation of roughness of machined surfaces using vision system by phase shifting method
If we keep doing what weâre doing weâll keep getting what weâre getting: A need to rethink âacademicâ medicine
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