Method for improving the signal-to-noise ratio of the Wheatstone bridge type bolometer Patent

Development of method for improving signal to noise ratio and accuracy of Wheatstone bridge type radiation measuring instrumen

Infrared horizon locator

A precise method and apparatus for locating the earth's infrared horizon from space that is independent of season and latitude is described. First and second integrations of the earth's radiance profile are made from space to earth with the second delayed with respect to the first. The second integration is multiplied by a predetermined constant R and then compared with the first integration. When the two are equal the horizon is located

Led induced chlorophyll fluorescence transient imager for measurements of health and stress status of whole plants

We have developed LED (light emitting diode) induced fluorescence transient imaging instrumentation to image the plant health/stress status by calculation of two images: Fv/Fm (variable fluorescence over saturation level of fluorescence) and the time response, tTR, of the fluorescence time curve. Within a short time interval (˜580 ms) multiple images (typically 20) are captured using the LEDs in the pulsed mode. For each pixel of the fluorescence image Fv/Fm and tTR are calculated and presented as images that correlate with the quantum yield of PSII photochemistry and the time response of this process, respectively. The advantage of the technology lies in the imaging of photosynthetic parameters within a short time interval, remotely and under light conditions. This was accomplished by the development of a high intensity pulsed LED light source (total 5 kW electrical power) and using the LEDs in the pulsed mode with a pulse width of 15 ms and time between sequential pulses of 14 ms. Using this instrumentation we investigated the effect of herbicide treatment, Sencor, on black nightshade (Solanum nigrum L.) plants. Effects of the herbicide on the first fluorescence images could be detected. At the saturation level of the fluorescence this effect disappeared. The effect of the herbicide was visualized on the Fv/Fm image and the time response tTR image. Healthy and herbicide treated parts of the plant yielded average values of Fv/Fm=0.81±0.03 and 0.06±0.02, respectively. Furthermore, the effect of drought stress was investigated on saintpaulia (Saintpaulia ionantha) plants. Under dark conditions no differences in the image of Fv/Fm and tTR could be detected between the control and the plant with drought stress. Under actinic light of 90 µmol m-2 s-1 differences were observed in images of (Fm’-F’)/Fm’ and tTR’. We conclude that for the first time images of a time response of the photosynthesis of leaves are presented. Furthermore, the proposed instrumentation can be used for high throughput screening, as a sensor in sorting machines and has potential greenhouse applications

Spatial-frequency response of the limb infrared monitor of the stratosphere

Measurements from the Limb Infrared Monitor of the stratosphere (LIMS) are presented and discussed. From the LIMS design model data, the modulation transfer function (MTF) was calculated for the optical system, the detector field mask, the electronics, and the overall system for each channel. The signal output performance of the instrument was predicted from the system MTF data and model input radiance data for each channel. The MTF measurements made on the flight sensor confirmed the analytical results

Brain organoids: Filling the need for a human model of neurological disorder

Neurological disorders are among the leading causes of death worldwide, accounting for almost all onsets of dementia in the elderly, and are known to negatively affect motor ability, mental and cognitive performance, as well as overall wellbeing and happiness. Currently, most neurological disorders go untreated due to a lack of viable treatment options. The reason for this lack of options is s poor understanding of the disorders, primarily due to research models that do not translate well into the human in vivo system. Current models for researching neurological disorders, neurodevelopment, and drug interactions in the central nervous system include in vitro monolayer cell cultures, and in vivo animal models. These models have shortcomings when it comes to translating research about disorder pathology, development, and treatment to humans. Brain organoids are three-dimensional (3D) cultures of stem cell-derived neural cells that mimic the development of the in vivo human brain with high degrees of accuracy. Researchers have started developing these miniature brains to model neurodevelopment, and neuropathology. Brain organoids have been used to model a wide range of neurological disorders, including the complex and poorly understood neurodevelopmental and neurodegenerative disorders. In this review, we discuss the brain organoid technology, placing special focus on the different brain organoid models that have been developed, discussing their strengths, weaknesses, and uses in neurological disease modeling

Production of organic seeds: Status, Challenges and Prospects

General article on the requirements of organic agriculture for seed production. Beside this the organic agricultural system has other demands for organic seed since it does not use chemical control measures and uses natural fertilizers instead of chemical fertilizers. Research can offer an important contribution in the production of seed without diseases

Mogelijkheden om vroeg tijdig bladrandproblemen te signaleren met MIPS bij Hortensia

Met geavanceerde camera technieken zijn beelden vast te leggen van fotosynthese activiteit en het is ook mogelijk gebleken om bladgedeelten met stress vast te leggen, zonder dat deze stress met het blote oog al te zien is. Dit opent perspectieven om monitorringonderzoek te doen naar het ontstaan van bladrandschade en tegelijkertijd naar de eventuele mogelijkheden tot ingrijpen met (wellicht) herstel. Deze metingen worden in het donker uitgevoerd. Er is recentelijk een nieuwe camera ontwikkeld die ook in het licht kan meten, maar bij een meting in het licht zal het verschil tussen schade- en gezonde plekken waarschijnlijk kleiner zijn dan bij een meting in het donker. Dit laatste zal getest moeten worden

Correcting Confocal Acquisition to Optimize Imaging of Fluorescence Resonance Energy Transfer by Sensitized Emission

AbstractImaging of fluorescence resonance energy transfer (FRET) between suitable fluorophores is increasingly being used to study cellular processes with high spatiotemporal resolution. The genetically encoded Cyan (CFP) and Yellow (YFP) variants of Green Fluorescent Protein have become the most popular donor and acceptor pair in cell biology. FRET between these fluorophores can be imaged by detecting sensitized emission. This technique, for which CFP is excited and transfer is detected as emission of YFP, is sensitive, fast, and straightforward, provided that proper corrections are made. In this study, the detection of sensitized emission between CFP and YFP by confocal microscopy is optimized. It is shown that this FRET pair is best excited at 430nm. We identify major sources of error and variability in confocal FRET acquisition including chromatic aberrations and instability of the excitation sources. We demonstrate that a novel correction algorithm that employs online corrective measurements yields reliable estimates of FRET efficiency, and it is also shown how the effect of other error sources can be minimized

Waterberging en natuur in het beekdal van de Beerze : veldwerkplaats Beekdallandschap Boxtel, 30 juni 2008

Beekdalen zijn heel geschikt om tijdelijk overtollig water op te slaan, om het vervolgens geleidelijk te laten afvloeien. Daarom worden vele beekdalen voor dit doel heringericht. Tegelijkertijd zijn de beekdalen echter vaak onderdeel van Natura 2000 of de EHS en daar gelden dus natuurdoelen. Zo ook het beekdal van de Beerze. De beek is op veel plaatsen verondiept, versmald, van vistrappen voorzien, meandert weer en een deel van het beekdal doet dienst als waterberging. Maar door de waterberging van het voedselrijke en soms verontreinigde water lukt het veelal niet de natuurdoelen te behalen. Zo’n situatie komt op veel meer plaatsen in Nederland voor. In dit verslag de ervaringen van Boxtel