7 research outputs found
Povezanost serumske koncentracije vitamina d i glaukoma kod žena
The aim of the study was to determine whether serum vitamin D level is lower in female patients with glaucoma as compared with control group. The mechanism by which vitamin D reduces intraocular pressure is not fully clarified. Almost all tissues possess vitamin D receptor (VDR). The mice lacking VDR (VDR knockout mice) have greatly contributed to the understanding of the general vitamin D physiologic function. VDR has been found in some ganglion layer cells, external and internal nuclear layers of retina, and in retinal pigment epithelium, while VDR epitopes have also been found in the ciliary body epithelium, pointing to the role of this protein in eye physiology. The 1,25(OH)2D3 modulates expression of the genes involved in the regulation of intraocular pressure in non-human primates. Extracellular matrix can be remodeled by 1,25(OH)2D3 treatment. Actin disruption can lead to cell morphology alteration, trabecular meshwork relaxation and intraocular pressure reduction. This observational cross-sectional study included 90 female glaucoma subjects aged 45-55 and 50 glaucoma free female subjects as control group. Results of a pilot study conducted in 20 glaucoma subjects and 20 control subjects are presented below. All study subjects underwent history taking, complete ophthalmologic examination and serum vitamin D determination. The mean serum vitamin D level was 32.31 nmol/L in glaucoma patients and 64.17 nmol/L in control subjects. Serum vitamin D level was statistically significantly lower in glaucoma patients as compared with control group (p<0.05).Cilj istraživanja bio je utvrditi je li kod ispitanica oboljelih od glaukoma snižena serumska koncentracija vitamina D u odnosu na kontrolnu skupinu. Mehanizam kojim vitamin D snižava oÄni tlak nije jasno poznat. Gotovo sva tkiva posjeduju receptor za vitamin D (VDR). Velik doprinos razumijevanju globalne fizioloÅ”ke funkcije vitamina D dobiven je od miÅ”a kojemu nedostaje VDR (engl. knockout mice for VDR). VDR je pronaÄen u nekim stanicama ganglijskog sloja, u vanjskom i unutarnjem nuklearnom sloju mrežnice i u retinalnom pigmentnom epitelu. Epitopi za VDR su pronaÄeni i u epitelu cilijarnog
tijela. To sve govori o važnosti tog proteina u fiziologiji oka. 1,25 (OH)2D3 modulira ekspresiju gena koji su ukljuÄeni u regulaciju oÄnog tlaka u nehumanih primata. Nakon lijeÄenja pomoÄu 1,25(OH)2D3 može se remodelirati ekstracelularni matriks. Disrupcija aktina može dovesti do promjene staniÄne morfologije, relaksacije trabekularne mreže i sniženja oÄnog tlaka. Provedeno je opažajno presjeÄno istraživanje s kontrolnom skupinom. U istraživanje je bilo ukljuÄeno 90 ispitanica oboljelih od glaukoma u dobi od 45-55 godina i 50 ispitanica u kontrolnoj skupini. Prikazuju se rezultati probnog ispitivanja u 20 ispitanica oboljelih od glaukoma i isto toliko u kontrolnoj skupini. Ispitanicama je uzeta anamneza i kompletan oftalmoloÅ”ki status te je odreÄen vitamin D u serumu. Koncentracija vitamina D u serumu ispitanica s glaukomom bila je 32,31 nmol/L u odnosu na kontrolnu skupinu gdje je bila 64,17 nmol/L. U zakljuÄku, kod ispitanica oboljelih od glaukoma bila je snižena serumska koncentracija vitamina D u odnosu na kontrolnu skupinu na statistiÄki znaÄajnoj razini od p<0,05
Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes
This study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTSā+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical-scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 Āµmol Lā1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical-scavenging efficacy
Antibacterial Fractions from Erodium cicutarium ExposedāClinical Strains of Staphylococcus aureus in Focus
Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strainsā
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the ātimeākillā
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from aboveādescribed methods led to further activityā
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloylāshikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloylāshikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigatio
Association between serum vitamin d level and glaucoma in women
The aim of the study was to determine whether serum vitamin D level is lower in female patients with glaucoma as compared with control group. The mechanism by which vitamin D reduces intraocular pressure is not fully clarified. Almost all tissues possess vitamin D receptor (VDR). The mice lacking VDR (VDR knockout mice) have greatly contributed to the understanding of the general vitamin D physiologic function. VDR has been found in some ganglion layer cells, external and internal nuclear layers of retina, and in retinal pigment epithelium, while VDR epitopes have also been found in the ciliary body epithelium, pointing to the role of this protein in eye physiology. The 1,25(OH)2D3 modulates expression of the genes involved in the regulation of intraocular pressure in non-human primates. Extracellular matrix can be remodeled by 1,25(OH)2D3 treatment. Actin disruption can lead to cell morphology alteration, trabecular meshwork relaxation and intraocular pressure reduction. This observational cross-sectional study included 90 female glaucoma subjects aged 45-55 and 50 glaucoma free female subjects as control group. Results of a pilot study conducted in 20 glaucoma subjects and 20 control subjects are presented below. All study subjects underwent history taking, complete ophthalmologic examination and serum vitamin D determination. The mean serum vitamin D level was 32.31 nmol/L in glaucoma patients and 64.17 nmol/L in control subjects. Serum vitamin D level was statistically significantly lower in glaucoma patients as compared with control group (p<0.05)
Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes
This study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTSā+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical-scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 Āµmol Lā1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical-scavenging efficacy
Fitokemijska karakterizacija bioaktivnih polifenolnih spojeva endemiÄne vrste Centaurea ragusina L. (Asteraceae)
Phytochemical characterization of the Croatian endemic
species Centaurea ragusina L. (Asteraceae) was carried out. The presence
of polyphenols in above ground plant parts was proven by using chemical
reactions of developing colored products and precipitates. The thin layer
chromatography showed the presence of quercetin, rutin, and chlorogenic
acid in the methanolic extract of C. ragusina. Quantitative analysis of total
polyphenols (TP), tannins (T), fiavonoids (F), and phenolic acids (PA) was
carried out using three different spectrophotometric methods. The contents of
analyzed polyphenolic compounds were as follows: 3.04Ā±0.04% (TP),
0.36Ā±0.04% (T), 0.25Ā±0.00% (F), and 0.38Ā±0.01% (PA). This phytochemical
characterization is a contribution to the scientific study of Croatian
endemic species and completes the existing knowledge about phytotherapeutic
potential of C. ragusina, especially in relation to the content of
bioactive polyphenolic substances
First Extensive Polyphenolic Profile of Erodium cicutarium with Novel Insights to Elemental Composition and Antioxidant Activity
Erodium cicutarium is known for its total polyphenolic content, but this work reveals the first highly detailed profile of E. cicutarium, obtained with UHPLC-LTQ OrbiTrap MS4 and UHPLC-QqQ-MS/MS techniques. A total of 85 phenolic compounds were identified and 17 constituents were quantified. Overall, 25 new compounds were found, which have not yet been reported for the Erodium genera, or the family Geraniaceae. Along with methanolic extracts, the so far poorly investigated water extracts exhibited in vitro antioxidant activity according to all performed assays, including the ferric reducing/antioxidant power assay (FRAP), 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), 2,2ā²-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) assay (ABTS) and cupric ion reducing antioxidant capacity assay (CUPRAC). Elemental composition analysis performed with inductively coupled plasma atomic emission spectrometry (ICP-AES) and, additionally, hydride generation atomic absorption spectrometry (HydrEA-ETAAS) showed six most abundant elements to be decreasing as follows: Mg>Ca>K>S>P>Na, and gave first data regarding inorganic arsenic content (109.3ā248.4 ng gā1). These results suggest E. cicutarium to be a valuable source of various phenolic compounds with substantial potential for further bioactivity testing