DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF GALLIC ACID, CURCUMIN AND PIPERINE IN AN AYURVEDIC FORMULATION

Objective: The objective of the present work was to establish a simple, precise, accurate and robust method for simultaneous estimation of gallic acid, curcumin and piperine from the marketed ayurvedic formulation by liquid chromatography. Methods: The separation was carried out on Hemochrom C18 Column (250 mm × 4.6 mm ID, 5 µm pore size) with a mobile phase methanol: acetonitrile: water (pH 3.2adjusted by using orthophosphate acid) in the ratio 70:20:10v/v by isocratic elution modeat 25 °C and the flow rate was setat0.8 ml/min. The analysis was carried out atisoabsorptive wavelength of 295 nm. Results: The retention time of gallic acid, curcumin and piperine was found to be 3.3(±0.2), 4.7 (±0.2) and 5.6 (±0.2) min, respectively. The linearity range for gallic acid, curcumin and piperine was found to be 10-70 μg/ml, 20-80 µg/ml and 2-14 µg/ml, respectively with the coefficient of linear regression greater than 0.99 for all markers. Mean percent recoveries for gallic acid, curcumin, and piperine were found within the limit of acceptance (99-100%). The percent relative standard deviation (%RSD) for precision and robustness was found less than 2%, which indicates the method is precise and robust. The developed method applied for quantification of these markers from the marketed ayurvedic formulation of Dekofcyn tablet. Conclusion: The developed method was found to be simple, rapid, precise and reproducible for standardization of Dekofcyn tablet and can be useful for other formulations containing these three markers

Nosodes and Sarcodes

158-163Nosodes and sarcodes are potentized preparations which are prepared according to homeopathic standards. Nosodes are prepared from inactivated diseased products of human, animal or vegetable origin or cultures of micro-organisms. In contrast, the source material for sarcodes comes from a healthy tissue, organ or secretion of humans or animals. As nosodes and sarcodes are prepared from body substance and discharge they have a peculiar affinity with the functions of the body and they are unique. Both nosodes and sarcodes act as prophylactics and can also be used for improvement of resistance, response to a remedy and for recuperation. The homeopathic nosodes can be used for treating residual infections (e.g. Bacillinum for tuberculosis) and as a prophylactic (e.g. Influenzium for swine flu) whereas sarcodes are used to help rebuild organs and tissues that may be diseased or malfunctioning (e.g. lymph, spleen)

Evaluation of Shaddharana churna: An Ayurvedic formulation

Shaddharana churna is a well-known Ayurvedic formulation that finds its reference in renowned Ayurvedic treaties such as Ashtangasangraham and Ashtanghridaya. As the name suggests Shaddharana churna is composed of equal parts of six drugs namely Katuka, Daruharidra, Ativisha, Chitraka, Patha, and Indravaya. Evaluation of Ayurvedic formulations is necessary to ensure their quality, strength, purity, and authenticity. Present work deals with microscopic, physico-chemical, qualitative, and quantitative evaluation of Shaddharana churna. Qualitative and quantitative evaluation was done by development and validation of high performance thin layer chromatography using berberine from Daruharidra, apocynin from Katuka and plumbagin from Chitraka as marker compounds. The outcomes of the research conform to the need of ensuring quality and safety of Ayurvedic medicines

Evaluation of <i>Shaddharana churna</i>: An <i>Ayurvedic</i> formulation

301-309Shaddharana churna is a well-known Ayurvedic formulation that finds its reference in renowned Ayurvedic treaties such as Ashtangasangraham and Ashtanghridaya. As the name suggests Shaddharana churna is composed of equal parts of six drugs namely Katuka, Daruharidra, Ativisha, Chitraka, Patha, and Indravaya. Evaluation of Ayurvedic formulations is necessary to ensure their quality, strength, purity, and authenticity. Present work deals with microscopic, physico-chemical, qualitative, and quantitative evaluation of Shaddharana churna. Qualitative and quantitative evaluation was done by development and validation of high performance thin layer chromatography using berberine from Daruharidra, apocynin from Katuka and plumbagin from Chitraka as marker compounds. The outcomes of the research conform to the need of ensuring quality and safety of Ayurvedic medicines