Cytotoxicity and DNA damage in the neutrophils of patients with sickle cell anaemia treated with hydroxyurea

Hydroxyurea (HU) is the most important advance in the treatment of sickle cell anaemia (SCA) for preventing complications and improving quality of life for patients. However, some aspects of treatment with HU remain unclear, including their effect on and potential toxicity to other blood cells such as neutrophils. This study used the measurement of Lactate Dehydrogenase (LDH) and Methyl ThiazolTetrazolium (MTT) and the comet assay to investigate the cytotoxicity and damage index (DI) of the DNA in the neutrophils of patients with SCA using HU.In the LDH and MTT assays, a cytoprotective effect was observed in the group of patients treated, as well as an absence of toxicity. When compared to patients without the treatment, the SS group (n=20, 13 women and 07 men, aged 18-69 years), and the group of healthy individuals (AA) used as a control group (n=52, 28 women and 24 men, aged 19-60 years), The SSHU group (n=21, 11 women and 10 men, aged 19-63 years) showed a significant reduction (p20 months), demonstrating that despite the cytoprotective effects in terms of cell viability, the use of HU can induce DNA damage in neutrophils

Search for charm in 250 GeV/c pipisup−sup -p interactions

A study was made of the reaction $pi$$sup -$ + p at 250 GeV/c in order to search for charmed particles. Cross sections, effective mass distributions, and an upper limit for each decay mode were found. However, no evidence was found for charmed particles. (JFP

Avaliação radiográfica do hálux valgo: estudo populacional de novos parâmetros angulares Radiographic evaluation of the hallux valgus: population study of new angular parameters

Estimulados pelo crescente interesse, pelos fatores intrínsecos na gênese da deformidade do hálux valgo, os autores estudaram os ângulos articulares distal do primeiro metatársico (AADM), articular proximal (AAPFP) e articular distal da falange proximal do hálux (AADFP). Para isso, avaliaram as radiografias na incidência AP em ortostase de 70 indivíduos brasileiros adultos e normais (140 pés), com idades variando de 18 a 55 anos (média 29 anos), distribuídos entre 40 indivíduos do sexo feminino (57,1%) e 30 do masculino (42,9%). A análise estatística a que se submeteram os dados indicaram como limites superiores da normalidade para a população estudada os valores de 8 graus para o AADN, 8 graus para o AAPFP e 10 graus para o AADFP.<br>Stimulated by the growing interest in the intrinsic factors at the genesis of the deformity of the hallux valgus, the authors studied the distal articular angle of the first metatarsal (DMAA), the proximal articular (PFPAA), and the distal articular of the proximal phalanx of the hallux (PFDAA). To do so, they evaluated the AP incidence of 70 grownup and normal Brazilian individual (140 feet) in orthostatic position with ages ranging from 18 to 55 years (29 in the average), 40 of which (57.1%) were female and 30 (42.9%) were male. The statistical analysis to wich the data were submitted pointed out as upper limts of normality, for the population studied, the following figures: 8 degrees for DMAA, 8 degrees for PFPAA and 10 degrees for PFDAA