Interleukin-16 inhibits sodium channel function and GluA1 phosphorylation via CD4- and CD9-independent mechanisms to reduce hippocampal neuronal excitability and synaptic activity

Interleukin 16 (IL-16) is a cytokine that is primarily associated with CD4+ T cell function, but also exists as a multi-domain PDZ protein expressed within cerebellar and hippocampal neurons. We have previously shown that lymphocyte-derived IL-16 is neuroprotective against excitotoxicity, but evidence of how it affects neuronal function is limited. Here, we have investigated whether IL-16 modulates neuronal excitability and synaptic activity in mouse primary hippocampal cultures. Application of recombinant IL-16 impairs both glutamate-induced increases in intracellular Ca2+ and sEPSC frequency and amplitude in a CD4- and CD9-independent manner. We examined the mechanisms underlying these effects, with rIL-16 reducing GluA1 S831 phosphorylation and inhibiting Na+ channel function. Taken together, these data suggest that IL-16 reduces neuronal excitability and synaptic activity via multiple mechanisms and adds further evidence that alternative receptors may exist for IL-16

Large-scale production of cellulose-binding domains : adsorption studies using CBD-FITC conjugates

A method for the gram-scale production of cellulose-binding domains (CBD) through the proteolytic digestion of a commercial nzymatic preparation (Celluclast) was developed. The CBD obtained, isolated from Trichoderma reesei cellobiohydrolase I, is highly pure and heavily glycosylated. The purified peptide has a molecular weight of 8.43 kDa, comprising the binding module, a part of the linker, and about 30% glycosidic moiety. Its properties may thus be different from recombinant ones expressed in bacteria. CBDfluorescein isothiocyanate conjugates were used to study the CBD-cellulose interaction. The presence of fluorescent peptides adsorbed on crystalline and amorphous cellulose fibers suggests that amorphous regions have a higher concentration of binding sites. The adsorption is reversible, but desorption is a very slow process.Fundação para a Ciência e a Tecnologia (FCT

Research article: Coastal flood-risk management practice in tai o, a town in Hong Kong

Coastal megadeltas in Asia have emerged rapidly; their megacities are particularly stressed by urbanization and rapid population growth. In the Pearl River Delta, towns in coastal megacities, such as Hong Kong, experience severe land shortage, which has led to the installation of essential infrastructure in flood-prone areas. Floods from the storm surge of two recent storms, Typhoons Hagupit and Koppu in 2008 and 2009, respectively, damaged over 100 properties in the Tai O, Hong Kong. Although projected sea-level rise and frequent storms pose a serious threat for the foreseeable future, flood management is inadequately understood by the various stakeholders in Tai O. Based on interviews of 22 stakeholders related to flood-risk management in Tai O, this article highlights the similarities and differences in the prevailing perceptions of coastal flood management practices. In addition, the report highlights the need for urgent attention so as to establish an integrated coastal flood-risk management strategy that will involve all stakeholders in mitigating the emerging flood vulnerability of the coastal communities. © 2013 National Association of Environmental Professionals