Identification of New SRF Binding Sites in Genes Modulated by SRF Over-Expression in Mouse Hearts

Background To identify in vivo new cardiac binding sites of serum response factor (SRF) in genes and to study the response of these genes to mild over-expression of SRF, we employed a cardiac-specific, transgenic mouse model, with mild over-expression of SRF (Mild-O SRF Tg). Methodology Microarray experiments were performed on hearts of Mild-O-SRF Tg at 6 months of age. We identified 207 genes that are important for cardiac function that were differentially expressed in vivo. Among them the promoter region of 192 genes had SRF binding motifs, the classic CArG or CArG-like (CArG-L) elements. Fifty-one of the 56 genes with classic SRF binding sites had not been previously reported. These SRF-modulated genes were grouped into 12 categories based on their function. It was observed that genes associated with cardiac energy metabolism shifted toward that of carbohydrate metabolism and away from that of fatty acid metabolism. The expression of genes that are involved in transcription and ion regulation were decreased, but expression of cytoskeletal genes was significantly increased. Using public databases of mouse models of hemodynamic stress (GEO database), we also found that similar altered expression of the SRF-modulated genes occurred in these hearts with cardiac ischemia or aortic constriction as well. Conclusion and significance SRF-modulated genes are actively regulated under various physiological and pathological conditions. We have discovered that a large number of cardiac genes have classic SRF binding sites and were significantly modulated in the Mild-O-SRF Tg mouse hearts. Hence, the mild elevation of SRF protein in the heart that is observed during typical adult aging may have a major impact on many SRF-modulated genes, thereby affecting Cardiac structure and performance. The results from our study could help to enhance our understanding of SRF regulation of cellular processes in the aged heart

Digestão do feno de capim-elefante anão (Pennisetum purpureum Schum. cv. Mott) sob diferentes níveis de consumo em ovinos Dwarf elephant grass hay (Pennisetum purpureum Schum. cv. Mott) digestion by sheep at different levels of intake

Foram utilizados doze ovinos Texel &times; Corriedale machos, castrados, com 12 meses de idade e peso vivo (PV) inicial médio de 27kg, mantidos em gaiolas de metabolismo, em um delineamento inteiramente casualizado, conduzido em dois períodos, para avaliar o efeito do nível de consumo (base matéria seca (MS)) de feno de capim elefante anão (CEA) (1,50; 1,75; 2,00; 2,25; 2,50% do PV e ad libitum) sobre a digestão nesses animais. A digestibilidade aparente da MS, da matéria orgânica (MO) e do nitrogênio (N), bem como a digestibilidade verdadeira do N, o teor de nutrientes digestíveis totais e a síntese de proteína microbiana ruminal não foram afetados, mas a retenção de N (em g dia-1 e como % do N consumido) foi sempre positiva e aumentou linearmente (P<0,01) com o aumento do consumo de CEA. No entanto, o aumento do consumo diminuiu linearmente a digestibilidade da fibra em detergente neutro (FDN), da fibra em detergente ácido (FDA) (P<0,05) e a digestibilidade verdadeira da MO (P<0,01). A taxa de passagem e o tempo de retenção no ceco-cólon não foram afetados, mas a taxa de passagem da digesta pelo retículo-rúmen aumentou e o tempo de retenção das partículas no retículo-rúmen (TRrr) e no trato digestivo total diminuiu (TRT) (P<0,05) com o aumento no consumo de feno. A redução do tempo de retenção do alimento no retículo-rúmen diminuiu a digestibilidade verdadeira da matéria orgânica e da parede celular da forragem. No entanto, não influenciou a digestibilidade aparente da matéria orgânica e, desse modo, não influenciou o valor energético da forragem.<br>Twelve one year old Texel x Corriedale castrated male sheep, with 27kg mean live weight (LW), housed in metabolic cages, were used in a completely randomized experiment, carried out in two periods, to evaluate the effect of level of intake (dry matter (DM) basis) of dwarf elephant grass (1.5; 1.75; 2.0; 2.25; 2.5% of LW and ad libitum) on digestion. DM, organic matter (OM) and nitrogen (N) apparent digestibility, as well as the N true digestibility, total digestible nutrients (TDN) contents and rumen microbial protein synthesis were not affected by level of hay intake. N retention (as g day-1 and as % of N intake) was always positive and increased linearly (P<0.05) as the level of hay intake increased. However, neutral (NDF) and acid detergent fiber (ADF) apparent digestibility, as well as OM true digestibility decreased linearly (P<0.05) with increasing intake. Passage rate and mean retention time in the cecum-colon were not affected, whereas passage rate through the reticulum-rumen increased and mean retention time decreased (P<0.05) as the level of intake increased. Fibre and OM true digestibility decreased as hay intake increased (P<0.05), mainly due to a decreasing in retention time of particles into the reticulo-rumen. However, OM apparent digestibility and, thus, the energetic value of dwarf elephant grass hay was not affected by the level of intake

Safety of hospital discharge before return of bowel function after elective colorectal surgery

Background: Ileus is common after colorectal surgery and is associated with an increased risk of postoperative complications. Identifying features of normal bowel recovery and the appropriateness for hospital discharge is challenging. This study explored the safety of hospital discharge before the return of bowel function. Methods: A prospective, multicentre cohort study was undertaken across an international collaborative network. Adult patients undergoing elective colorectal resection between January and April 2018 were included. The main outcome of interest was readmission to hospital within 30 days of surgery. The impact of discharge timing according to the return of bowel function was explored using multivariable regression analysis. Other outcomes were postoperative complications within 30 days of surgery, measured using the Clavien\u2013Dindo classification system. Results: A total of 3288 patients were included in the analysis, of whom 301 (9\ub72 per cent) were discharged before the return of bowel function. The median duration of hospital stay for patients discharged before and after return of bowel function was 5 (i.q.r. 4\u20137) and 7 (6\u20138) days respectively (P &lt; 0\ub7001). There were no significant differences in rates of readmission between these groups (6\ub76 versus 8\ub70 per cent; P = 0\ub7499), and this remained the case after multivariable adjustment for baseline differences (odds ratio 0\ub790, 95 per cent c.i. 0\ub755 to 1\ub746; P = 0\ub7659). Rates of postoperative complications were also similar in those discharged before versus after return of bowel function (minor: 34\ub77 versus 39\ub75 per cent; major 3\ub73 versus 3\ub74 per cent; P = 0\ub7110). Conclusion: Discharge before return of bowel function after elective colorectal surgery appears to be safe in appropriately selected patients