53 research outputs found
Albumin-derived peptides efficiently reduce renal uptake of radiolabelled peptides
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88022.pdf (publisher's version ) (Closed access)PURPOSE: In peptide-receptor radionuclide therapy (PRRT), the maximum activity dose that can safely be administered is limited by high renal uptake and retention of radiolabelled peptides. The kidney radiation dose can be reduced by coinfusion of agents that competitively inhibit the reabsorption of radiolabelled peptides, such as positively charged amino acids, Gelofusine, or trypsinised albumin. The aim of this study was to identify more specific and potent inhibitors of the kidney reabsorption of radiolabelled peptides, based on albumin. METHODS: Albumin was fragmented using cyanogen bromide and six albumin-derived peptides with different numbers of electric charges were selected and synthesised. The effect of albumin fragments (FRALB-C) and selected albumin-derived peptides on the internalisation of (111)In-albumin, (111)In-minigastrin, (111)In-exendin and (111)In-octreotide by megalin-expressing cells was assessed. In rats, the effect of Gelofusine and albumin-derived peptides on the renal uptake and biodistribution of (111)In-minigastrin, (111)In-exendin and (111)In-octreotide was determined. RESULTS: FRALB-C significantly reduced the uptake of all radiolabelled peptides in vitro. The albumin-derived peptides showed different potencies in reducing the uptake of (111)In-albumin, (111)In-exendin and (111)In-minigastrin in vitro. The most efficient albumin-derived peptide (peptide #6), was selected for in vivo testing. In rats, 5 mg of peptide #6 very efficiently inhibited the renal uptake of (111)In-minigastrin, by 88%. Uptake of (111)In-exendin and (111)In-octreotide was reduced by 26 and 33%, respectively. CONCLUSIONS: The albumin-derived peptide #6 efficiently inhibited the renal reabsorption of (111)In-minigastrin, (111)In-exendin and (111)In-octreotide and is a promising candidate for kidney protection in PRRT.1 februari 201
The effects of potato virus Y-derived virus small interfering RNAs of three biologically distinct strains on potato (Solanum tuberosum) transcriptome
The epigenetic modifier JMJD6 is amplified in mammary tumors and cooperates with c-Myc to enhance cellular transformation, tumor progression, and metastasis
The AF-1-deficient estrogen receptor ERα46 isoform is frequently expressed in human breast tumors
Production froide des huiles visqueuses. Première partie : Observations sur champ
La production froide de certains réservoirs contenant des huiles visqueuses, au Canada et au Venezuela principalement, amène à des productivités et des taux de récupération supérieurs aux prédictions calculées à l'aide des équations habituellement utilisées pour décrire les écoulements classiques. Pour expliquer ces productions anormalement élevées, un certain nombre de mécanismes ont été évoqués, principalement d'origines hydrodynamiques et géomécaniques. Les propriétés hydrodynamiques des fluides produits, partiellement dégazés durant la production : huiles moussantes, fluides à bulles, sont en effet plus favorables que celles des fluides en place. Par effet géomécanique d'érosion interne du sable, un réseau de chenaux (wormholes, piping tubes) peut se créer in situ, améliorant notablement le drainage. Cet article comprend quatre parties : les observations sur champ, l'étude en laboratoire des huiles moussantes, leur modélisation numérique, les effets géomécaniques liés à la production de sable
Rt-Pcr-Elosa Tests On Pooled Sample Units For The Detection Of Virus Y In Potato Tubers
Strong resistance to potato tuber necrotic ringspot disease in potato induced by transformation with coat protein gene sequences from an NTN isolate of Potato virus Y
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