Gonadotropina kosmówkowa jako kluczowy czynnik regulujący implantację zarodka

Abstract Chorionic gonadotropin (CG), as a embryonic factor, controls the implantation process and pregnancy formation. The hormone regulates endometrial functions by its influence on progesterone production and direct modulation of endometrial cell functions. Recent studies suggest the key role of CG in controlling the main implantation processes like apoptosis, angiogenesis, maternal immunological response and trophoblast migration. The review of recent studies regarding CG function indicates its pivotal role in embryo implantation and suggests possible medical applications.Streszczenie Gonadotropina kosmówkowa (CG), jako czynnik zarodkowy, kontroluje proces implantacji i formowania się ciąży. Hormon ten reguluje funkcje błony śluzowej macicy zarówno poprzez wpływ na produkcję progesteronu, jak i poprzez bezpośrednie oddziaływanie na komórki endometrium. Badania ostatnich lat wskazują na kluczowa rolę CG w kontroli najważniejszych procesów związanych z implantacja, takich jak apoptoza, angiogeneza, matczyna odpowiedź immunologiczna i migracja trofoblastu. Przegląd piśmiennictwa ostatnich lat wskazuje na decydującą rolę gonadotropiny kosmówkowej w procesie formowania się ciąży i sugeruje potencjalne zastosowanie medyczne tego hormonu.

Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor beta 1

Chronic kidney disease (CKD) is common in geriatric cats, and the most prevalent pathology is chronic tubulointerstitial inflammation and fibrosis. The cell type predominantly responsible for the production of extra-cellular matrix in renal fibrosis is the myofibroblast, and fibroblast to myofibroblast differentiation is probably a crucial event. The cytokine TGF-β1 is reportedly the most important regulator of myofibroblastic differentiation in other species. The aim of this study was to isolate and characterise renal fibroblasts from cadaverous kidney tissue of cats with and without CKD, and to investigate the transcriptional response to TGF-β1

Quality of life and satisfaction with life in SLE patients—the importance of clinical manifestations

To assess the correlation between quality of life (QoL) and satisfaction with life (SL) in SLE patients and correlate both with clinical symptoms of the disease. The study was performed in 83 patients. QoL was assessed by Short Form 36, and SL was assessed by the Satisfaction with Life Scale. Clinical manifestations presented at the time of examination were taken into consideration. SLE patients assessed their QoL and SL as rather low. Those with photosensitivity as well as neurological symptoms presented lower QoL in particular domains, while those with renal manifestation of SLE assessed their QoL as higher. Similar observations were made for SL only in relation to neurological symptoms. Moreover, our findings show that although SL is a part of QoL, both these parameters should be distinguished in order to fully assess the state of the patient

Modulation of transforming growth factor beta signalling pathway genes by transforming growth factor beta in human osteoarthritic chondrocytes: involvement of Sp1 in both early and late response cells to transforming growth factor beta

International audienc

Expression of Human Frataxin Is Regulated by Transcription Factors SRF and TFAP2

Friedreich ataxia is an autosomal recessive neurodegenerative disease caused by reduced expression levels of the frataxin gene (FXN) due to expansion of triplet nucleotide GAA repeats in the first intron of FXN. Augmentation of frataxin expression levels in affected Friedreich ataxia patient tissues might substantially slow disease progression.We utilized bioinformatic tools in conjunction with chromatin immunoprecipitation and electrophoretic mobility shift assays to identify transcription factors that influence transcription of the FXN gene. We found that the transcription factors SRF and TFAP2 bind directly to FXN promoter sequences. SRF and TFAP2 binding sequences in the FXN promoter enhanced transcription from luciferase constructs, while mutagenesis of the predicted SRF or TFAP2 binding sites significantly decreased FXN promoter activity. Further analysis demonstrated that robust SRF- and TFAP2-mediated transcriptional activity was dependent on a regulatory element, located immediately downstream of the first FXN exon. Finally, over-expression of either SRF or TFAP2 significantly increased frataxin mRNA and protein levels in HEK293 cells, and frataxin mRNA levels were also elevated in SH-SY5Y cells and in Friedreich ataxia patient lymphoblasts transfected with SRF or TFAP2.We identified two transcription factors, SRF and TFAP2, as well as an intronic element encompassing EGR3-like sequence, that work together to regulate expression of the FXN gene. By providing new mechanistic insights into the molecular factors influencing frataxin expression, our results should aid in the discovery of new therapeutic targets for the treatment of Friedreich ataxia

MicroRNAs Differentially Expressed in Postnatal Aortic Development Downregulate Elastin via 3′ UTR and Coding-Sequence Binding Sites

Elastin production is characteristically turned off during the maturation of elastin-rich organs such as the aorta. MicroRNAs (miRNAs) are small regulatory RNAs that down-regulate target mRNAs by binding to miRNA regulatory elements (MREs) typically located in the 3′ UTR. Here we show a striking up-regulation of miR-29 and miR-15 family miRNAs during murine aortic development with commensurate down-regulation of targets including elastin and other extracellular matrix (ECM) genes. There were a total of 14 MREs for miR-29 in the coding sequences (CDS) and 3′ UTR of elastin, which was highly significant, and up to 22 miR-29 MREs were found in the CDS of multiple ECM genes including several collagens. This overrepresentation was conserved throughout mammalian evolution. Luciferase reporter assays showed synergistic effects of miR-29 and miR-15 family miRNAs on 3′ UTR and coding-sequence elastin constructs. Our results demonstrate that multiple miR-29 and miR-15 family MREs are characteristic for some ECM genes and suggest that miR-29 and miR-15 family miRNAs are involved in the down-regulation of elastin in the adult aorta

Microbiological indicators for sanitary soil contamination near leaking cesspool

Celem pracy było określenie wskaźników mikrobiologicznych pozwalających potwierdzić zjawisko nieszczelności szamba, przez skażenie pobliskiej gleby ściekami komunalnymi. Z próbek ścieków i gleby wyizolowano i zidentyfikowano kilka gatunków bakterii, stanowiących potencjalne sanitarne zagrożenie dla środowiska: Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Clostridium difficile, Streptococcus uberis.The main purpose of this thesis was to determine the microbiological indicators confirm that the phenomenon of leakages cesspool, contamination of nearby soil by municipal sewage. In the sewage samples and the soil were isolated and identified several species of bacteria, representing a potential health hazard: Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Clostridium difficile, Streptococcus uberis

Sensitivity of predatory bacteria of the genus Bdellovibrio sp. and their preys from Enterobacteriaceae family on antibiotics and disinfectant

Celem pracy była ocena możliwości wykorzystania bakterii drapieżnych z rodzaju Bdellovibrio do oczyszczania ścieków komunalnych z patogennych bakterii Serratia liquefaciens i Citrobacter freundii. Wykazano, że wyizolowane ze ścieków bakterie drapieżne oraz ich ofiary były wrażliwe na antybiotyki, m.in. chloramfenikol, streptomycynę i tetracyklinę oraz płyn dezynfekcyjny tzw. Wodę Ecofair. Stwierdzono, że bakterie Bdellovibrio sp. mogą pełnić funkcję regulatora liczebności bakterii G (-) z rodziny Enterobacteriaceae.The aim of this study was to evaluate the use of predatory bacteria of the genus Bdellovibrio to treatment municipal wastewater with Serratia liquefaciens and Citrobacter freundii bacteria. It was observed, that isolated predadatory bacteria and their preys were sensitive on antibiotics: chloramphenicol, streptomycine and tetracycline and Ecofair Water disinfectant. It was showed that the bacteria of Bdellovibrio genus can reduce the total number of G (-) pathogenic bacteria from Enterobacteriaceae family