Combined Electrical Resistivity Imaging and Electromagnetic Survey for Groundwater Studies in the Tarkwa Mining Area, Ghana

The major source of potable water in Tarkwa is the Bonsa Treatment Plant sourced from the Bonsa River. The activities of illegal miners along the banks of the Bonsa River has resulted in pollution of the river. This has resulted in high treatment cost and irregular supply of water to the Tarkwa Township and surrounding communities that are fed by the Bonsa Treatment Plant. In view of the difficulty in getting frequent and regular potable supply of water, people have resorted to construction of boreholes and hand-dug wells. However, the success rates and borehole yields are low especially in the hydrogeologically difficult terrains in the Tarkwa area. The aim of this paper is to investigate the hydrogeological conditions of the Tarkwa area using both the Electrical Resistivity Imaging (ERI) and Electromagnetic (EM) geophysical techniques to determine the electrical resistivity and conductivity values that are related to groundwater accumulation, so that potential water-bearing zones can be identified. Results from electrical resistivity show that the general resistivity distribution in the Tarkwa area is between 32 Ωm and 100 000 Ωm. Water-bearing zones in the Huni Sandstone occur to a depth of 35 m with an average resistivity value of 400 Ωm, at a depth of 60 m and a resistivity of 600 Ωm in the Tarkwa Phyllite, at a depth of 55 m and a resistivity of 600 Ωm in the Banket Series and 50 m depth with resistivity value of 500 Ωm in the Kawere Conglomerate respectively. The electromagnetic conductivity values also show that the general conductivity distribution in the Tarkwa area is 3 – 32 S/m. The application of electrical resistivity and electromagnetic techniques separately gives success rate of 80 % and 65 % respectively. An improved success rate of 86 % is achieved combining the two techniques. Keywords: Electrical Resistivity Imaging, Electromagnetic Method, Groundwate

Groundwater Vulnerability Assessment of the Tarkwa Mining Area Using SINTACS Approach and GIS

Groundwater vulnerability assessment to delineate areas that are susceptible to contamination from mining and anthropogenic activities has become an important element for resource management and landuse planning. In view of the extensive mining in the Tarkwa area, quality of groundwater has become an important issue. This study estimates aquifer vulnerability by applying the SINTACS model which uses seven environmental parameters to evaluate aquifer vulnerability and geographical information system (GIS) in the Tarkwa mining area. Sensitivity analysis has also been carried out to evaluate the relative importance of the model parameters for aquifer vulnerability. The SINTACS model results show that the intrusive rocks within the Tarkwaian and the Birimian rocks are dominated by very high vulnerability classes while the Banket Series is characterised by high vulnerability class. The Huni Sandstones have moderately high vulnerability. In addition, the Kawere Group and the Tarkwa Phyllites displayed medium vulnerability. Analysis from the variogram model shows that all parameters used in the SINTACS model have a strong spatial structure. From statistical analysis, depth to water parameter inflicted the highest impact on the vulnerability of the aquifer followed by effective infiltration, vadose zone media, soil media, aquifer media, topography and hydraulic conductivity in the order of decreasing impact. Sensitivity analysis indicated that the aquifer media, hydraulic characteristics and topography cause large variation in vulnerability index. Depth to water and effective infiltration were found to be more effective in assessing aquifer vulnerability. Keywords: Groundwater, Vulnerability, Tarkwa, SINTACS, GI

Estimation of the Quantity of Water in the Abandoned Underground Mine of Gold Fields Ghana Limited Tarkwa: A Potential Source to Augment Water Supply to Tarkwa Municipality

The Tarkwa district is an important gold mining area in the Southwestern part of Ghana. The main source of potable water supply to the Tarkwa Nsuaem Municipality is from the Bonsa River treatment plant managed by the Ghana Water Company Limited (GWCL). The River is under threat from serious contamination by illegal mining ("galamsey") activities within its catchment area. Consequently, the amount of water supplied to the Municipality has not kept pace with its growing population due to increasing treatment cost and supply difficulties. The need to find alternative and sustainable sources of potable water supply to augment that from GWCL to the Municipality has become imperative. A large void volume created as a result of the abandoned underground mine operated by Gold Fields Ghana Limited (GFGL), after its closure in 1999 has flooded. This potential water resource is being pumped out daily, and wasted, sometimes spilling-over to low lying areas around the mine when allowed to reach its decant level. This study estimated the quantity of water in the Abontiakoon Vertical Shaft (AVS) which is part of the large underground void using survey production figures and post-closure void filling parameters resulting in 2.8 x 106 m3 and 2.9 x 106 m3 respectively. The rate of recharge to the underground water was also estimated to ascertain the sustainability of the void water should it be considered for use by employing the model of predicting rebound on “void filling” basis and average dewatering rate before closure at 2 535 m3/day and 2 618 m3/day respectively; indicating that recharge to the AVS reservoir is about 6 x 106 gal/day or 30% of current daily water supply deficit in the TNM. The estimated potential volume of mine water in storage in the entire Tarkwa underground void is 32 x106 m3. Two samples of the mine water were taken in November 2011 and February 2015 for quality analysis, in order to have a fair knowledge of the water quality parameters. The quality of the underground water was found to be potentially good, and not likely to cause any health threats, or water quality problems. Depth sampling is recommended to determine the chemical profile of the reservoir. Keywords: Reservoir, Municipality, Bonsa River, Contamination, Tarkw

Product Marking and Conformity Assessment of Portland Cements on the Ghanaian Market

Cement bound concrete materials and complementary fittings are requisite ingredients for all civil engineering works. In all these, Portland cement, a basic binding ingredient for the concrete work is the dominant binder. In Ghana, there are various brands of cement on the market. Five major brand products currently in circulation include the Ghana Cement (GHACEM), Western DIAMOND Cement (DIAMOND), CIMAF Cement, DANGOTE Cement and SUPACEM Cement. Increased infrastructural development has placed high demand on cement consumption. Consequently, new products keep emerging in the market. Indeed, a standard measure to provide product marking and evaluations of conformity to standard Class thresholds are required for the desired specification, properties and the performance quality of the cement products. This research therefore sets to ascertain the strength quality of the five cement brands on the Ghanaian market by checking their conformity to C-30 and C-40 standard compressive tests, using their 32.5-R and 42.5-R flagship brands. To achieve this, concrete cubes were moulded with fixed mix ratio of 1:1⅟2:3 and 1:1:2 for C-30 and C-40 respectively. To achieve the desired strength conformity, the slump as well as the coarse and fine aggregate constituents were standardised. The results indicated that the cement brands despite parading same strength thresholds in the market, do not exhibit same strength build-up. There are significant variations in growth of compressive strength over time. It was observed also that conformance threshold within 28 days was not attained for a number of the brands. Indeed, not until 56 days or more some of the brands could not achieve their desired compressive strength thresholds

The Geometry and Structural Analysis of the Gold Deposits of Chirano Mine

The Chirano Mine gold deposit is a typical example of a structurally controlled deposit developed along the Kumasi Basin and the Sefwi Belt margin structure. The area has undergone various regimes of structural deformations. Consequently, all the Chirano deposits are intimately associated with shears and faults along a single continuous structural corridor known as the Chirano Shear Zone (CSZ). The CSZ geometry has been categorised into three major zones namely: (i) Laminated veins in shears, (ii) Breccia and (iii) Ductile to brittle ductile zones. The shear veins trend NE-SW and N-S, are laminated and occur in the sheared fabric close to the footwall. Penetrative foliated zones varying from a few centimeters to several meters constitute the ductile to brittle-ductile structures. Gold grades are much higher within this zone. Analysis of cataclasis intensity recorded in drill core confirms a semi brittle form of deformation within the mineralised domain. The CSZ has different orientations in dip and strike from the south of the mining lease to the north. The subtle changes in orientation are deposit dependent. The structure has a sinuous shape and tends to pinch and swell. The current geometry and the distribution of stratigraphy and orebodies at Chirano is a manifestation of the complex interplay of magmatic and hydrothermal events in the area.  Keywords: Ductile, Brittle-Ductile, Breccia, Chirano Shear Zone, Chirano Lode Horizo

DRAM-3 modulates autophagy and promotes cell survival in the absence of glucose

Macroautophagy is a membrane-trafficking process that delivers cytoplasmic constituents to lysosomes for degradation. The process operates under basal conditions as a mechanism to turnover damaged or misfolded proteins and organelles. As a result, it has a major role in preserving cellular integrity and viability. In addition to this basal function, macroautophagy can also be modulated in response to various forms of cellular stress, and the rate and cargoes of macroautophagy can be tailored to facilitate appropriate cellular responses in particular situations. The macroautophagy machinery is regulated by a group of evolutionarily conserved autophagy-related (ATG) proteins and by several other autophagy regulators, which either have tissue-restricted expression or operate in specific contexts. We report here the characterization of a novel autophagy regulator that we have termed DRAM-3 due to its significant homology to damage-regulated autophagy modulator (DRAM-1). DRAM-3 is expressed in a broad spectrum of normal tissues and tumor cells, but different from DRAM-1, DRAM-3 is not induced by p53 or DNA-damaging agents. Immunofluorescence studies revealed that DRAM-3 localizes to lysosomes/autolysosomes, endosomes and the plasma membrane, but not the endoplasmic reticulum, phagophores, autophagosomes or Golgi, indicating significant overlap with DRAM-1 localization and with organelles associated with macroautophagy. In this regard, we further proceed to show that DRAM-3 expression causes accumulation of autophagosomes under basal conditions and enhances autophagic flux. Reciprocally, CRISPR/Cas9-mediated disruption of DRAM-3 impairs autophagic flux confirming that DRAM-3 is a modulator of macroautophagy. As macroautophagy can be cytoprotective under starvation conditions, we also tested whether DRAM-3 could promote survival on nutrient deprivation. This revealed that DRAM-3 can repress cell death and promote long-term clonogenic survival of cells grown in the absence of glucose. Interestingly, however, this effect is macroautophagy-independent. In summary, these findings constitute the primary characterization of DRAM-3 as a modulator of both macroautophagy and cell survival under starvation conditions

Investigating the role of c-Jun N-terminal kinases in the proliferation of Werner syndrome fibroblasts using diaminopyridine inhibitors

Fibroblasts derived from the progeroid Werner syndrome show reduced replicative lifespan and a "stressed" morphology, both alleviated using the MAP kinase inhibitor SB203580. However, interpretation of these data is problematical because although SB203580 has the stress-activated kinases p38 and JNK1/2 as its preferred targets, it does show relatively low overall kinase selectivity. Several lines of data support a role for both p38 and JNK1/2 activation in the control of cellular proliferation and also the pathology of diseases of ageing, including type II diabetes, diseases to which Werner Syndrome individuals are prone, thus making the use of JNK inhibitors attractive as possible therapeutics. We have thus tested the effects of the widely used JNK inhibitor SP600125 on the proliferation and morphology of WS cells. In addition we synthesised and tested two recently described aminopyridine based inhibitors. SP600125 treatment resulted in the cessation of proliferation of WS cells and resulted in a senescent-like cellular phenotype that does not appear to be related to the inhibition of JNK1/2. In contrast, use of the more selective aminopyridine CMPD 6o at concentrations that fully inhibit JNK1/2 had a positive effect on cellular proliferation of immortalised WS cells, but no effect on the replicative lifespan of primary WS fibroblasts. In addition, CMPD 6o corrected the stressed WS cellular morphology. The aminopyridine CMPD 6r, however, had little effect on WS cells. CMDP 6o was also found to be a weak inhibitor of MK2, which may partially explain its effects on WS cells, since MK2 is known to be involved in regulating cellular morphology via HSP27 phosphorylation, and is thought to play a role in cell cycle arrest. These data suggest that total JNK1/2 activity does not play a substantial role in the proliferation control in WS cells

Impaired Autophagy of an Intracellular Pathogen Induced by a Crohn's Disease Associated ATG16L1 Variant

The genetic risk factors predisposing individuals to the development of inflammatory bowel disease are beginning to be deciphered by genome-wide association studies. Surprisingly, these new data point towards a critical role of autophagy in the pathogenesis of Crohn's disease. A single common coding variant in the autophagy protein ATG16L1 predisposes individuals to the development of Crohn's disease: while ATG16L1 encoding threonine at amino acid position 300 (ATG16L1*300T) confers protection, ATG16L1 encoding for alanine instead of threonine (ATG16L1*300A, also known as T300A) mediates risk towards the development of Crohn's disease. Here we report that, in human epithelial cells, the Crohn's disease-associated ATG16L1 coding variant shows impairment in the capture of internalized Salmonella within autophagosomes. Thus, we propose that the association of ATG16L1*300A with increased risk of Crohn's disease is due to impaired bacterial handling and lowered rates of bacterial capture by autophagy

Neuregulin Promotes Incomplete Autophagy of Prostate Cancer Cells That Is Independent of mTOR Pathway Inhibition

Growth factors activating the ErbB receptors have been described in prostate tumors. The androgen dependent prostate cancer cell line, LNCaP, expresses the ErbB-1, ErbB-2 and ErbB-3 receptor tyrosine kinases. Previously, it was demonstrated that NRG activates ErbB-2/ErbB-3 heterodimers to induce LNCaP cell death, whereas, EGF activates ErbB-1/ErbB-1 or ErbB-1/ErbB-2 dimers to induce cell growth and survival. It was also demonstrated that PI3K inhibitors repressed this cell death suggesting that in androgen deprived LNCaP cells, NRG activates a PI3K-dependent pathway associated with cell death.In the present study we demonstrate that NRG induces autophagy in LNCaP cells, using LC3 as a marker. However, the autophagy induced by NRG may be incomplete since p62 levels elevate. We also demonstrated that NRG- induced autophagy is independent of mammalian target of rapamycin (mTOR) inhibition since NRG induces Akt and S6K activation. Interestingly, inhibition of reactive oxygen species (ROS) by N-acetylcysteine (NAC), inhibited NRG-induced autophagy and cell death. Our study also identified JNK and Beclin 1 as important components in NRG-induced autophagy and cell death. NRG induced elevation in JNK phosphorylation that was inhibited by NAC. Moreover, inhibitor of JNK inhibited NRG-induced autophagy and cell death. Also, in cells overexpressing Bcl-2 or cells expressing sh-RNA against Beclin 1, the effects of NRG, namely induction of autophagy and cell death, were inhibited.Thus, in LNCaP cells, NRG-induces incomplete autophagy and cell death that depend on ROS levels. These effects of NRG are mediated by signaling pathway that activates JNK and Beclin 1, but is independent of mTOR inhibition