Reproductive wastage among Merino ewe flocks. 2. Non-clover areas

This research sought to define the reasons for low fertility among Merino ewes in Western Australia. This first article looks at wastage in Non-Clover area

Chronic contractile dysfunction without hypertrophy does not provoke a compensatory transcriptional response in mouse hearts

Diseased myocardium from humans and experimental animal models shows heightened expression and activity of a specific subtype of phospholipase C (PLC), the splice variant PLCβ1b. Previous studies from our group showed that increasing PLCβ1b expression in adult mouse hearts by viral transduction was sufficient to cause sustained contractile dysfunction of rapid onset, which was maintained indefinitely in the absence of other pathological changes in the myocardium. We hypothesized that impaired contractility alone would be sufficient to induce a compensatory transcriptional response. Unbiased, comprehensive mRNA-sequencing was performed on 6 biological replicates of rAAV6-treated blank, PLCβ1b and PLCβ1a (closely related but inactive splice variant) hearts 8 weeks after injection, when reduced contractility was manifest in PLCβ1b hearts without evidence of induced hypertrophy. Expression of PLCβ1b resulted in expression changes in only 9 genes at FDR<0.1 when compared with control and these genes appeared unrelated to contractility. Importantly, PLCβ1a caused similar mild expression changes to PLCβ1b, despite a complete lack of effect of this isoform on cardiac contractility. We conclude that contractile depression caused by PLCβ1b activation is largely independent of changes in the transcriptome, and thus that lowered contractility is not sufficient in itself to provoke measurable transcriptomic alterations. In addition, our data stress the importance of a stringent control group to filter out transcriptional changes unrelated to cardiac function

Effects of Altering Supplementation Frequency During the Pre-Partum Period of Beef Cows Grazing Dormant Native Range

Spring-calving beef cattle that are grazing low-quality (\u3c 7% crude protein) dormant forage typically are unable to meet their maintenance requirements for protein. Providing a protein supplement (\u3e 30% crude protein) is recommended to decrease losses of body weight and body condition. Nutrient supplementation when forage quality is poor or limited is one of the largest expenditures for forage-based beef cattle operations. The expansion of the ethanol industry has afforded many producers in corn and sorghum-producing regions an alternative to traditional oilseed-based protein supplements. The availability and nutrient profile of distiller’s grains with solubles (DDG) has made it popular as a supplement for cows that are grazing dormant lowquality forages. Decreasing supplementation frequency reduces costs for labor and fuel. Previous research has shown no difference in body weights or body condition scores of cows supplemented with distiller’s grains daily, once every three days, or once every six days; however, the proportion of cows that ate hay during the 60 minutes immediately after supplementation was less on the day of supplementation for cows supplemented once every six days compared to cows supplemented daily. The observed decrease in hay consumption following supplementation could potentially decrease total organic matter intake during late gestation. More frequent supplementation may increase organic matter intake and improve performance during the month before parturition. Therefore, the objective of this study was to evaluate effects of altering frequency of supplementing distiller’s grains during the last 28 days of gestation with respect to performance of spring-calving beef cows consuming low-quality dormant native range

Performance of Beef Replacement Heifers Supplemented With Dried Distillers Grains With Solubles Versus a Mixture of Soybean Meal and Finely Ground Sorghum Grain

Costs of feed, labor, and equipment to develop heifers in a traditional confined feeding system are relatively high. Producers can greatly reduce input costs by developing heifers on dormant native range; however, heifers are unable to consume sufficient protein from the forage base. Suboptimal protein intake will reduce forage digestion and result in poor performance. Supplemental protein is required during these times to increase forage intake and digestion. The most efficient way of providing supplemental protein to heifers consuming lowquality (\u3c7% crude protein) forage is by feeding high-protein supplements (\u3e30% crude protein). Producers traditionally have used high-quality oilseed meals to supplement the necessary protein, but expansion of the ethanol industry has made dried distillers grains with solubles (DDGS) a potentially less expensive supplement option for producers in corn- and sorghum-producing areas. The objective of this study was to evaluate the effects of daily supplementation of DDGS vs. a mixture of soybean meal and finely ground sorghum grain on performance of replacement heifers grazing low-quality dormant native range

Performance of Beef Replacement Heifers Supplemented with Dried Distillers Grains or a Mixture of Soybean Meal and Ground Sorghum Grain

The feed, labor, and equipment costs of developing heifers in a confined feeding system are relatively high. High Plains beef producers can reduce input costs by developing heifers on dormant native range; however, heifers are typically unable to consume sufficient crude protein (CP) from the low-quality (\u3c 7% CP) forage base. Insufficient dietary protein reduces forage digestion and performance potential of growing heifers. Supplementing protein when forage quality was poor has previously been reported to increase forage intake and forage digestibility, which resulted in acceptable levels of performance. An efficient means of supplying supplemental protein to heifers consuming low-quality forage is through the use of supplements with relatively high crude protein concentrations (\u3e 30% CP). Traditionally, producers have used oilseed meals in this capacity, but with the expansion of the ethanol industry, dried distillers grains with solubles (DDG) have become widely available as an alternative protein source for producers in corn and sorghum-producing regions. Adequate heifer body weight and body condition score at first breeding are essential to minimize age at first calving and to increase lifetime productivity. Therefore, the objective of our study was to evaluate the effects of supplementation of DDG or an approximately isonitrogenous mixture of soybean meal and ground sorghum grain on growth and reproductive performance of replacement heifers grazing low-quality, dormant native range

Effects of Altering Supplementation Frequency During the Pre-Partum Period of Beef Cows Grazing Dormant Native Range

Spring-calving beef cattle that are grazing low-quality (\u3c 7% crude protein) dormant forage typically are unable to meet their maintenance requirements for protein. Providing a protein supplement (\u3e 30% crude protein) is recommended to decrease losses of body weight and body condition. Nutrient supplementation when forage quality is poor or limited is one of the largest expenditures for forage-based beef cattle operations. The expansion of the ethanol industry has afforded many producers in corn and sorghum-producing regions an alternative to traditional oilseed-based protein supplements. The availability and nutrient profile of distiller’s grains with solubles (DDG) has made it popular as a supplement for cows that are grazing dormant low-quality forages. Decreasing supplementation frequency reduces costs for labor and fuel. Previous research has shown no difference in body weights or body condition scores of cows supplemented with distiller’s grains daily, once every three days, or once every six days; however, the proportion of cows that ate hay during the 60 minutes immediately after supplementation was less on the day of supplementation for cows supplemented once every six days compared to cows supplemented daily. The observed decrease in hay consumption following supplementation could potentially decrease total organic matter intake during late gestation. More frequent supplementation may increase organic matter intake and improve performance during the month before parturition. Therefore, the objective of this study was to evaluate effects of altering frequency of supplementing distiller’s grains during the last 28 days of gestation with respect to performance of spring-calving beef cows consuming low-quality dormant native range

Factors Associated with Recurrence of Varicose Veins after Thermal Ablation: Results of The Recurrent Veins after Thermal Ablation Study

Background. The goal of this retrospective cohort study (REVATA) was to determine the site, source, and contributory factors of varicose vein recurrence after radiofrequency (RF) and laser ablation. Methods. Seven centers enrolled patients into the study over a 1-year period. All patients underwent previous thermal ablation of the great saphenous vein (GSV), small saphenous vein (SSV), or anterior accessory great saphenous vein (AAGSV). From a specific designed study tool, the etiology of recurrence was identified. Results. 2,380 patients were evaluated during this time frame. A total of 164 patients had varicose vein recurrence at a median of 3 years. GSV ablation was the initial treatment in 159 patients (RF: 33, laser: 126, 52 of these patients had either SSV or AAGSV ablation concurrently). Total or partial GSV recanalization occurred in 47 patients. New AAGSV reflux occurred in 40 patients, and new SSV reflux occurred in 24 patients. Perforator pathology was present in 64% of patients. Conclusion. Recurrence of varicose veins occurred at a median of 3 years after procedure. The four most important factors associated with recurrent veins included perforating veins, recanalized GSV, new AAGSV reflux, and new SSV reflux in decreasing frequency. Patients who underwent RF treatment had a statistically higher rate of recanalization than those treated with laser

Gott Time Machines, BTZ Black Hole Formation, and Choptuik Scaling

We study the formation of BTZ black holes by the collision of point particles. It is shown that the Gott time machine, originally constructed for the case of vanishing cosmological constant, provides a precise mechanism for black hole formation. As a result, one obtains an exact analytic understanding of the Choptuik scaling.Comment: 6 pages, Late

Edge pixel response studies of edgeless silicon sensor technology for pixellated imaging detectors

Silicon sensor technologies with reduced dead area at the sensor's perimeter are under development at a number of institutes. Several fabrication methods for sensors which are sensitive close to the physical edge of the device are under investigation utilising techniques such as active-edges, passivated edges and current-terminating rings. Such technologies offer the goal of a seamlessly tiled detection surface with minimum dead space between the individual modules. In order to quantify the performance of different geometries and different bulk and implant types, characterisation of several sensors fabricated using active-edge technology were performed at the B16 beam line of the Diamond Light Source. The sensors were fabricated by VTT and bump-bonded to Timepix ROICs. They were 100 and 200 μ m thick sensors, with the last pixel-to-edge distance of either 50 or 100 μ m. The sensors were fabricated as either n-on-n or n-on-p type devices. Using 15 keV monochromatic X-rays with a beam spot of 2.5 μ m, the performance at the outer edge and corners pixels of the sensors was evaluated at three bias voltages. The results indicate a significant change in the charge collection properties between the edge and 5th (up to 275 μ m) from edge pixel for the 200 μ m thick n-on-n sensor. The edge pixel performance of the 100 μ m thick n-on-p sensors is affected only for the last two pixels (up to 110 μ m) subject to biasing conditions. Imaging characteristics of all sensor types investigated are stable over time and the non-uniformities can be minimised by flat-field corrections. The results from the synchrotron tests combined with lab measurements are presented along with an explanation of the observed effects