Massive Parallel Current Power Amplifier Concept for Power Hardware in the Loop Applications

The development of the smartgrid increases the complexity of the current electric grid. To verify and validate the operation of the systems involved in it, Power Hardware-In-theLoop (PHIL) technique allows to test the complete system in an exhaustive way. But the reduced bandwidth of the overall test system can cause inaccuracies and instabilities, which can be harmful for the Hardware Under Test (HUT) or the people who are performing the test. To increase PHIL performance and tackle these problems, this paper proposes a new concept of high bandwidth current amplifier. It is based on a topology of massive parallel interleaved buck-boost converter, which distribute in an equal manner the total current in all the branches. This current reduction allows to use transistors with better switching behaviour, which increase the bandwidth of the converter. Furthermore, a Discontinuous Conduction Mode (DCM) is used, obtaining the nominal output current in only one switching cycle. Description of the concept and the design parameters are provided. Finally, the behaviour of the proposed Power Amplifier (PA) at high frequency setpoint currents is shown in a Matlab/Simulink simulation. © 2022, European Association for the Development of Renewable Energy, Environment and Power Quality (EA4EPQ). All rights reserved

Multi-technology battery storage system for optimal demand-side management

Demand-side management (DSM) provides additional system flexibility for the electric energy environment. To enable the spread of DSM policies the use of automatic systems combined with a local battery energy storage system (BESS) is proposed. Following this statement, it has been developed and tested a 50 kVA low-cost multi-technology energy storage system, with a novel converter topology that permits the integration of multiple battery types or aging. It consists of a 50 kVA bidirectional three-level AC/DC converter for grid connection which feeds five 10 kW isolated bi-directional DC/DC battery converters, based on SiC and Si MOSFETs. The development leads in a pick-and-place system, adding interoperability, ease of connection and modularity

A committed tissue-resident memory T cell precursor within the circulating CD8+ effector T cell pool

An increasing body of evidence emphasizes the role of tissue-resident memory T cells (TRM) in the defense against recurring pathogens and malignant neoplasms. However, little is known with regard to the origin of these cells and their kinship to other CD8+ T cell compartments. To address this issue, we followed the antigen-specific progeny of individual naive CD8+ T cells to the T effector (TEFF), T circulating memory (TCIRCM), and TRM pools by lineage-tracing and single-cell transcriptome analysis. We demonstrate that a subset of T cell clones possesses a heightened capacity to form TRM, and that enriched expression of TRM-fate-associated genes is already apparent in the circulating TEFF offspring of such clones. In addition, we demonstrate that the capacity to generate TRM is permanently imprinted at the clonal level, before skin entry. Collectively, these data provide compelling evidence for early stage TRM fate decisions and the existence of committed TRM precursor cells in the circulatory TEFF compartment.Toxicolog

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis

Although many aspects of blood production are well understood, the spatial organization of myeloid differentiation in the bone marrow remains unknown. Here we use imaging to track granulocyte/macrophage progenitor (GMP) behaviour in mice during emergency and leukaemic myelopoiesis. In the steady state, we find individual GMPs scattered throughout the bone marrow. During regeneration, we observe expanding GMP patches forming defined GMP clusters, which, in turn, locally differentiate into granulocytes. The timed release of important bone marrow niche signals (SCF, IL-1β, G-CSF, TGFβ and CXCL4) and activation of an inducible $\textit{Irf8}$ and β-catenin progenitor self-renewal network control the transient formation of regenerating GMP clusters. In leukaemia, we show that GMP clusters are constantly produced owing to persistent activation of the self-renewal network and a lack of termination cytokines that normally restore haematopoietic stem-cell quiescence. Our results uncover a previously unrecognized dynamic behaviour of GMPs $\textit{in situ}$, which tunes emergency myelopoiesis and is hijacked in leukaemia.This work was supported by NIH K01DK098315 award to E.M.P.; a Bloodwise and CRUK program grants and Wellcome Trust funding to the Cambridge Stem Cell Institute to B.G.; and NIH R01HL092471, R01HL111266 and P30DK063720 grants, Rita Allen Scholar Award and Leukemia Lymphoma Society Scholar Award to E.P

Synthesis and activity of inhibitors highly specific for the glycolytic enzymes from Trypanosoma brucei.

Most glycosomal enzymes of Trypanosoma brucei carry a relatively high number of positive charges. In at least 3 of the enzymes some of the charges unique to these enzymes are concentrated in 2 distinct areas on the enzymes' surface, about 4 nm apart [4] and these positively charged structural elements have been suggested to be the site of interaction with the trypanocidal drug Suramin. We have synthesized a series of symmetrical long chain molecules with negative charges or strong dipoles at each end. Several of these compounds inhibited the glycosomal enzymes more strongly than Suramin. They also exhibited a specificity for the trypanosome enzymes, when compared with homologous enzymes from other organisms. By varying the chain length of the active compounds, a 4-nm distance between the molecules' extremes proved optimal for inhibition. Tetra-substituted compounds were better than di-substituted. Modifications introduced at the two ends indicated that a planar orientation, with an amide bond linking a phenyl ring to the chain, is preferred. Inhibition kinetics for some of the enzymes indicated the existence of multi-site interactions with the inhibitors