Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane

p53 is a transcription factor that induces growth arrest or apoptosis in response to cellular stress. To identify new p53-inducible proapoptotic genes, we compared, by differential display, the expression of genes in spleen or thymus of normal and p53 nullizygote mice after γ-irradiation of whole animals. We report the identification and characterization of human and mouse Scotin homologues, a novel gene directly transactivated by p53. The Scotin protein is localized to the ER and the nuclear membrane. Scotin can induce apoptosis in a caspase-dependent manner. Inhibition of endogenous Scotin expression increases resistance to p53-dependent apoptosis induced by DNA damage, suggesting that Scotin plays a role in p53-dependent apoptosis. The discovery of Scotin brings to light a role of the ER in p53-dependent apoptosis

Tailored graph ensembles as proxies or null models for real networks I: tools for quantifying structure

We study the tailoring of structured random graph ensembles to real networks, with the objective of generating precise and practical mathematical tools for quantifying and comparing network topologies macroscopically, beyond the level of degree statistics. Our family of ensembles can produce graphs with any prescribed degree distribution and any degree-degree correlation function, its control parameters can be calculated fully analytically, and as a result we can calculate (asymptotically) formulae for entropies and complexities, and for information-theoretic distances between networks, expressed directly and explicitly in terms of their measured degree distribution and degree correlations.Comment: 25 pages, 3 figure

Milk as a drug delivery platform in paediatrics

Communication presented at the PSSRC − 9th Annual Symposium. 16-18 September 2015, Ghent, Belgium"Purpose: to assess the physical properties of spray dried milk powders and evaluate drug-milk interactions.

Medicinal powders made of fresh milk for pediatric use

Communication presented at the 1st International Congress of CiiEM - From Basic Sciences to Clinical Research. 27-28 November 2015, Egas Moniz, Caparica, Portugal"Nowadays, the need to formulate medicines specifically designed for children is imperative [1] and solid dosage forms are the first choice for providing the required drug stability and dose accuracy. Milk, as a worldwide accepted food, is proposed in this study as a platform to deliver drugs orally in pediatrics. Therefore, the main goals of this project are: (a) to assess the properties of spray-dried milk powders, (b) to evaluate drug-milk interactions and (c) to ascertain the stability of the spray-dried milk powders."Fundação para a Ciência e a Tecnologi

Optical and Near Infrared Study of the Cepheus E outflow, a very low excitation object

We present images and spectra of the Cepheus E (Cep E) region at both optical and infrared wavelengths. Only the brightest region of the southern lobe of the Cep E outflow reveals optical emission, suggesting that the extinction close to the outflow source plays an important r\^ole in the observed difference between the optical and IR morphologies. Cep E is a unique object since it provides a link between the spectroscopic properties of the optical Herbig-Haro (HH) objects and those of deeply embedded outflows.Comment: Accepted Astron. J., 8 files: paper, tables plus 6 figure

Visita precursora do projeto Rondon: relato de experiências no estado do Pará, em Santa Luzia do Pará, em 2007, e em São Domingos do Araguaia, em 2015

Trabalho apresentado no II Congresso Nacional do PROJETO RONDON, realizado em Florianópolis, SC, no período de 23 a 25 de setembro de 2015 - Universidade Federal de Santa Catarina.A visita precursora tem a finalidade de fazer o reconhecimento local, como manter contato com a comunidade e apoio dos órgãos públicos, assim como flexibilizar a proposta encaminhada nas condições do município, prepararem alojamento e alimentação para alunos e professores durante os dezesseis dias que permanecem nas Operações, escolhida para a IES atuar. No momento da visita precursora o Projeto Rondon sai da teoria e vai para a prática, além do que serve para transformar a proposta em plano de trabalho factível as localidades. Este artigo tem o objetivo de descrever as experiências na visita precursora nos Municípios de Santa Luzia do Pará, em 2007 e em São Domingos do Araguaia, em 2015, no Estado do Pará, como planejamento para a Operação Grão-Pará e para a operação Itacaiúnas, em dois momentos diferentes do Projeto Rondon. Foi realizada uma pesquisa bibliográfica descritiva e de relato de caso sobre a visita precursora em duas épocas do Projeto Rondon, durante a Operação Grão-Pará em 2007 e a Operação Itacaiúnas em 2015, ambas no Estado do Pará. Foi observado durante a pesquisa que visita precursora tem passado por muitas transformações, necessária as realidades do Projeto Rondon, das IES e principalmente dos Municípios envolvidos, é um instrumento de planejamento para que as Operações ocorram com eficiência e eficácia à época de sua realização. A vista precursora é a composição de cenários para a realização das Operações do Projeto Rondon as quais se destinam, até mesmo para manter contato com a comunidade, a fim de apoiar os Rondonistas, principalmente em se tratando da hospedagem e alimentação dos alunos e professores das equipes do conjunto A e B. Especificamente as visitas precursoras com Santa Luzia do Pará, destinadas as Operações Grão-Pará em 2007 e Itacaiúnas em 2015, foram infinitamente importantes para delinearmos e ajustarmos a proposta à realidade do município, até mesmo para organizarmos os minicursos, workshops, entre outras atividades, durante as Operações

Spray-dried fresh milk powders for oral drug delivery in pediatrics

Poster presented at the 10th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology. 4-7 April 2016, Glasgow, UKN/

Effects of hydroxycinnamic acids on the glycolysis pathway

Glycolysis is a metabolic pathway vital to the production of energy and some organisms rely on it solely to meet their energy requirements. It is also a central pathway in the metabolism of carbohydrates and a source of therapeutic targets against diabetes and cancer. Caffeoylquinic acids (CQAs) have been extensively studied for their role in the treatment and prevention of diabetes (and cancer) but their mechanisms of action remain mostly unknown. As such, molecular docking was used to find possible targets of CQAs in the glycolysis pathway. The molecular docking assays showed that CQAs were docked preferably to the Rossman fold (nicotinamide adenine dinucleotide — NAD(H) binding site) of oxidoreductases, that use NAD(H) as a cofactor, than to any other site. In-vitro assays were then performed using two NAD(H) dependent oxidoreductases from glycolysis (alcohol dehydrogenase and L-lactate dehydrogenase) in order confirm if CQAs would compete with the cofactor to inhibit the reaction. The results from these assays indicate that CQAs can act as both inhibitors and activators of NAD(H) dependent oxidoreductases of the glycolysis pathway.info:eu-repo/semantics/publishedVersio