7 research outputs found
A Spatially-Constrained Normalized Gamma Process for Data Clustering
Part 9: ClusteringInternational audienceIn this work, we propose a novel nonparametric Bayesian method for clustering of data with spatial interdependencies. Specifically, we devise a novel normalized Gamma process, regulated by a simplified (pointwise) Markov random field (Gibbsian) distribution with a countably infinite number of states. As a result of its construction, the proposed model allows for introducing spatial dependencies in the clustering mechanics of the normalized Gamma process, thus yielding a novel nonparametric Bayesian method for spatial data clustering. We derive an efficient truncated variational Bayesian algorithm for model inference. We examine the efficacy of our approach by considering an image segmentation application using a real-world dataset. We show that our approach outperforms related methods from the field of Bayesian nonparametrics, including the infinite hidden Markov random field model, and the Dirichlet process prior
Barley Yellow Mosaic Virus: Purification, Electron Microscopy, Serology, and other Properties of two Types of the Virus
Causal agent of sharka disease: historical perspective and current status of Plum pox virus strains
Classical and next generation sequencing approaches unravel <em>Bymovirus</em> diversity in barley crops in France.
Despite the generalized use of cultivars carrying the rym4 resistance gene, the impact of viral mosaic diseases on winter barleys increased in recent years in France. This change could reflect i) an increased prevalence of the rym4 resistance-breaking pathotype of Barley yellow mosaic virus Y (BaYMV-2), ii) the emergence of rym4 resistance-breaking pathotypes of Barley mild mosaic virus (BaMMV) or iii) the emergence of other viruses. A study was undertaken to determine the distribution and diversity of viruses causing yellow mosaic disease. A collection of 241 symptomatic leaf samples from susceptible, rym4 and rym5 varieties was gathered from 117 sites. The viruses present in all samples were identified by specific RT-PCR assays and, for selected samples, by double-stranded RNA next-generation sequencing (NGS). The results show that BaYMV-2 is responsible for the symptoms observed in varieties carrying the resistance gene rym4. In susceptible varieties, both BaYMV-1 and BaYMV-2 were detected, together with BaMMV. Phylogenetic analyses indicate that the rym4 resistance-breaking ability independently evolved in multiple genetic backgrounds. Parallel analyses revealed a similar scenario of multiple independent emergence events in BaMMV for rym5 resistance-breaking, likely involving multiple amino acid positions in the viral-linked genome protein. NGS analyses and classical techniques provided highly convergent results, highlighting and validating the power of NGS approaches for diagnostics and viral population characterization