Crystal structure of the 3C protease from South African Territories type 2 foot-and-mouth disease virus

The replication of foot-and-mouth disease virus (FMDV) is dependent on the virus-encoded 3C protease (3Cpro). As in other picornaviruses, 3Cpro performs most of the proteolytic processing of the polyprotein expressed from the single open reading frame in the RNA genome of the virus. Previous work revealed that the 3Cpro from serotype A – one of the seven serotypes of FMDV – adopts a trypsin-like fold. Phylogenetically the FMDV serotypes are grouped into two clusters, with O, A, C, and Asia 1 in one, and the three South African Territories serotypes, (SAT-1, SAT-2 and SAT-3) in another. We report here the cloning, expression and purification of 3C proteases from four SAT serotype viruses (SAT2/GHA/8/91, SAT1/NIG/5/81, SAT1/UGA/1/97, and SAT2/ZIM/7/83) and the crystal structure at 3.2 Å resolution of 3Cpro from SAT2/GHA/8/91)

Harbin: a quantitation PCR analysis tool

Objectives: To enable analysis and comparisons of different relative quantitation experiments, a web-browser application called Harbin was created that uses a quantile-based scoring system for the comparison of samples at different time points and between experiments. Results: Harbin uses the standard curve method for relative quantitation to calculate concentration ratios (CRs). To evaluate if different datasets can be combined the Harbin quantile bootstrap test is proposed. This test is more sensitive in detecting distributional differences between data sets than the Kolmogorov–Smirnov test. The utility of the test is demonstrated in a comparison of three grapevine leafroll associated virus 3 (GLRaV-3) RT-qPCR data sets. Conclusions: The quantile-based scoring system of CRs will enable the monitoring of virus titre or gene expression over different time points and be useful in other genomic applications where the combining of data sets are required

Terrestrial biological studies in Southern Victoria Land, Antarctica.

The biota, comprising the bryophyte flushes on continental Antarctica, are specialized organisms adapted to the rigorous conditions imposed on them by the environment. Ice free areas of continental Antarctica provide the few habitats suitable for colonization by plants and invertebrates. The presence of free water and certain climatic conditions are essential features. This report investigates bryophyte flushes in two ice free areas in Southern Victoria Land. The first is within the Lake Fryxell Site of Special Scientific Interest No.12 adjacent to the Canada Glacier in the Taylor Valley, one of the so called Dry Valleys. The second is at Granite Harbour approximately sixty kilometres north of the Taylor Valley site

Building audiences: Aboriginal and Torres Strait Islander arts

Building Audiences examines the barriers to and the strategies for increasing audiences in the Aboriginal and Torres Strait Islander arts sector. This research investigates the attitudes, beliefs and behaviours of current and potential audiences. What is in the report? The findings reveal the key barriers facing audience attendance include: uncertainty about how to behave at cultural events and fear of offending lack of awareness with audiences not actively seeking information about Indigenous arts and outdated perceptions of the sector – that it is only perceived as ‘serious or educational’. Building Audiences also considered several strategies to build audiences for Indigenous arts: providing skills development, advice and resourcing to Indigenous practitioners within the arts sector; increasing representation of Indigenous artists in the main programing of arts companies by including more Indigenous people in decision making roles; promoting relationships between Indigenous arts and non-Indigenous companies to present their work to wider audiences; introducing children and young people to Indigenous arts through schools and extracurricular activities; allowing audiences to feel comfortable engaging by creating accessible experiences; implementing long-term strategies to change negative perceptions of Indigenous arts. The project was commissioned by the Australia Council for the Arts and funding partners include Australia Council for the Arts; Faculty of Business and Law and Institute of Koorie Education, Deakin University; Melbourne Business School, The University of Melbourne

Teachers' perceptions of how attention-deficit/hyperactivity disorder may influence learners' career choices

This article reports on teachers’ perceptions of how ADHD may influence learners’ career choices. Purposive selection was used to identify six high school teachers who cater specifically for learners with Attention-Deficit/Hyperactivity Disorder (ADHD). An exploratory case study design and a qualitative approach were adopted. Data was gathered using an audio-recorded semi-structured focus group interview, and analysed and interpreted using inductive thematic analysis. Findings suggest teachers believe that ADHD affects parents, schooling, and the school environment from an early age, that taking medication for ADHD from an early age may positively and negatively influence affect learners’ career choices, and that intervention to address the longer-term impact of ADHD on learners’ career choices is essential. Moreover, teachers believe that parents play a powerful role in the career choice of learners with ADHD. Future research should include early childhood carers, teachers and other health professionals, and include larger and more diverse groups of participants

Selective serotonin reuptake inhibitors (SSRIs) for stroke recovery.

BACKGROUND: Stroke is the major cause of adult disability. Selective serotonin reuptake inhibitors (SSRIs) have been used for many years to manage depression. Recently, small trials have demonstrated that SSRIs might improve recovery after stroke, even in people who are not depressed. Systematic reviews and meta-analyses are the least biased way to bring together data from several trials. Given the promising effect of SSRIs on stroke recovery seen in small trials, a systematic review and meta-analysis is needed. OBJECTIVES: To determine whether SSRIs improve recovery after stroke, and whether treatment with SSRIs was associated with adverse effects. SEARCH METHODS: We searched the Cochrane Stroke Group Trials Register (August 2011), Cochrane Depression Anxiety and Neurosis Group Trials Register (November 2011), Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2011, Issue 8), MEDLINE (from 1948 to August 2011), EMBASE (from 1980 to August 2011), CINAHL (from 1982 to August 2011), AMED (Allied and Complementary Medicine) (from 1985 to August 2011), PsycINFO (from 1967 to August 2011) and PsycBITE (Pyschological Database for Brain Impairment Treatment Efficacy) (March 2012). To identify further published, unpublished and ongoing trials we searched trials registers, pharmaceutical websites, reference lists, contacted experts and performed citation tracking of included studies. SELECTION CRITERIA: We included randomised controlled trials that recruited stroke survivors (ischaemic or haemorrhagic) at any time within the first year. The intervention was any SSRI, given at any dose, for any period. We excluded drugs with mixed pharmacological effects. The comparator was usual care or placebo. In order to be included, trials had to collect data on at least one of our primary (dependence and disability) or secondary (impairments, depression, anxiety, quality of life, fatigue, healthcare cost, death, adverse events and leaving the trial early) outcomes. DATA COLLECTION AND ANALYSIS: We extracted data on demographics, type of stroke, time since stroke, our primary and secondary outcomes, and sources of bias. For trials in English, two review authors independently extracted data. For Chinese papers, one review author extracted data. We used standardised mean differences (SMD) to estimate treatment effects for continuous variables, and risk ratios (RR) for dichotomous effects, with their 95% confidence intervals (CIs). MAIN RESULTS: We identified 56 completed trials of SSRI versus control, of which 52 trials (4059 participants) provided data for meta-analysis. There were statistically significant benefits of SSRI on both of the primary outcomes: RR for reducing dependency at the end of treatment was 0.81 (95% CI 0.68 to 0.97) based on one trial, and for disability score, the SMD was 0.91 (95% CI 0.60 to 1.22) (22 trials involving 1343 participants) with high heterogeneity between trials (I(2) = 87%; P < 0.0001). For neurological deficit, depression and anxiety, there were statistically significant benefits of SSRIs. For neurological deficit score, the SMD was -1.00 (95% CI -1.26 to -0.75) (29 trials involving 2011 participants) with high heterogeneity between trials (I(2) = 86%; P < 0.00001). For dichotomous depression scores, the RR was 0.43 (95% CI 0.24 to 0.77) (eight trials involving 771 participants) with high heterogeneity between trials (I(2) = 77%; P < 0.0001). For continuous depression scores, the SMD was -1.91 (95% CI -2.34 to -1.48) (39 trials involving 2728 participants) with high heterogeneity between trials (I(2) = 95%; P < 0.00001). For anxiety, the SMD was -0.77 (95% CI -1.52 to -0.02) (eight trials involving 413 participants) with high heterogeneity between trials (I(2) = 92%; P < 0.00001). There was no statistically significant benefit of SSRI on cognition, death, motor deficits and leaving the trial early. For cognition, the SMD was 0.32 (95% CI -0.23 to 0.86), (seven trials involving 425 participants) with high heterogeneity between trials (I(2) = 86%; P < 0.00001). The RR for death was 0.76 (95% CI 0.34 to 1.70) (46 trials involving 3344 participants) with no heterogeneity between trials (I(2) = 0%; P = 0.85). For motor deficits, the SMD was -0.33 (95% CI -1.22 to 0.56) (two trials involving 145 participants). The RR for leaving the trial early was 1.02 (95% CI 0.86 to 1.21) in favour of control, with no heterogeneity between trials. There was a non-significant excess of seizures (RR 2.67; 95% CI 0.61 to 11.63) (seven trials involving 444 participants), a non-significant excess of gastrointestinal side effects (RR 1.90; 95% CI 0.94 to 3.85) (14 trials involving 902 participants) and a non-significant excess of bleeding (RR 1.63; 95% CI 0.20 to 13.05) (two trials involving 249 participants) in those allocated SSRIs. Data were not available on quality of life, fatigue or healthcare costs.There was no clear evidence from subgroup analyses that one SSRI was consistently superior to another, or that time since stroke or depression at baseline had a major influence on effect sizes. Sensitivity analyses suggested that effect sizes were smaller when we excluded trials at high or unclear risk of bias.Only eight trials provided data on outcomes after treatment had been completed; the effect sizes were generally in favour of SSRIs but CIs were wide. AUTHORS' CONCLUSIONS: SSRIs appeared to improve dependence, disability, neurological impairment, anxiety and depression after stroke, but there was heterogeneity between trials and methodological limitations in a substantial proportion of the trials. Large, well-designed trials are now needed to determine whether SSRIs should be given routinely to patients with stroke

Revisiting core issues in dynamic assessment

Dynamic assessment is currently poised at a juncture at which theoretical and practical assessment resolutions are necessitated. Such issues concern theoretical approaches towards  psycho-educational assessment.  In order to partially explore these basic assessment approaches, a questionnaire was delivered via electronic mail to one hundred internationally, currently active dynamic assessment researchers and practitioners. The findings from the responses formed the basis for an informal content analysis, which was conducted utilising themes as primary meaning unit and word counts as secondary meaning unit of analyses. The one common and uniting feature about the current research in this area is the broad range of theoretical approaches towards assessment and the current lack of unanimity across types of approaches. Responses showed that varied theoretical frameworks are employed in dynamic assessments which do not necessarily cohere with other traditional approaches. It is contended that an exploratory revisiting of core assessment approaches would assist in positioning practitioners’ and researchers’ theoretical approaches in future assessments

A review of South African research in the field of dynamic assessment

Dynamic assessment, which is often characterised by the learning potential approach across the world and in South Africa, is receiving more attention from educators and research practitioners alike. When compared to the status of international research, local dynamic assessment research can still be regarded as being in its infancy. A selection of studies conducted within this domain was analysed and the results carefully assessed in terms of positive and negative findings to serve as an indication of the trends that this discipline may face in South Africa. The main findings indicate that although the field is still being researched today, there has been a decrease in the number of studies as well as a concomitant decrease in the implementation of dynamic research efforts. The reasons cited are a lack of time, costs, inefficiencies and also confusion as to what dynamic assessment entails. There is, as yet, no consistent definition of dynamic assessment in South Africa, which makes it all the harder to entrench dynamic assessment as a methodology and implement it on as wide a scale as possible

Efficacy of using life design-based counselling for an emerging adult who had suffered parental neglect

This article reports on the value of life design-based counselling for an emerging adult who had experienced parental (emotional) neglect and was now ready to embark on a career. Purposive sampling was used to select an emerging adult who had experienced parental neglect and had entered the workplace stage. Such research in South African education contexts is limited. A QUAL + quan design was used to gather and analyse the data. An intrinsic, descriptive, exploratory intervention case study involving a single individual was the basis of the research. Thematic data analysis (in combination with ATLAS.ti) was conducted to analyse the data, that is, look for themes and subthemes in the data. The intervention enhanced the participant’s career resilience and career adaptability. Exploring her life story for themes and patterns helped her gain new insight into the meaning of her experiences. The intervention enabled her to enter a new chapter in her life from a position of strength and an improved sense of self. Longitudinal studies on the long-term effects of life design-based interventions on groups of emerging adults who had experienced different kinds of parental neglect are needed to determine the longer-term value of the kind of intervention described in this article