Contribuição da fixação simbiótica de nitrogênio na produção de guandu (Cajanus cajan (L.) Millsp).

Experimento com guandu utilizando solo Latossolo-vermelho-amarelo, variação Laras no Instituto de Zootecnia, Nova Odessa, para avaliar parâmetros indiretos de fixação simbiotica de N2. As plantas utilizadas representam acesso de guandu obtidos no CPPSE-EMBRAPA, São Carlos. Selecionados para caracterizadas agronomicas forrageiras. Matéria seca da parte aérea, matéria seca de nodulos

Sheep Grazing Patterns for Better Land Management: Adjusting GPS Tracking Protocol

Small ruminant livestock systems in northeast Portugal are an extensive activity based on daily grazing paths across the landscape. The flocks use multiple patches of multiple land cover types in different ways throughout the year. Shepherd and flock interactions determine the resting and feeding spots utilized by sheep and goats according to the biotic and abiotic conditions. Information about the herding home range is central to managing the land use and vegetation cover and optimizing sheep and goats\u27 productivity in traditional systems. This study\u27s main objective is to contribute to calibrate a shepherding GPS protocol to monitor sheep flocks based on fieldwork in Vimieiro (Mirandela) on a protected area of the European Natura 2000 network. We answer two farmers\u27 and breeders\u27 requests for using GPS collars to monitor the landscape usage by sheep: (1) How closely do collared sheep remain within the flock? (2) How do the collars perform on different logging frequencies to estimate patch occupancy? We analyzed the grazing paths based on three collars\u27 5-minute GPS positions from winter to summer solstices. We investigated the differences in extent, duration, and frequency data of each collar throughout the season change based on spatial regressions. Results show no significant differences among the three collars ranges. It also indicates that positions collected every 15 and 30 minutes could be adequate. It means that a flock monitoring low cost can be generalized, providing accurate information to manage the pastoral territory and increase the small ruminant\u27s productivit

Comparison of reproducibility, accuracy, sensitivity, and specificity of miRNA quantification platforms

Given the increasing interest in their use as disease biomarkers, the establishment of reproducible, accurate, sensitive, and specific platforms for microRNA (miRNA) quantification in biofluids is of high priority. We compare four platforms for these characteristics: small RNA sequencing (RNA-seq), FirePlex, EdgeSeq, and nCounter. For a pool of synthetic miRNAs, coefficients of variation for technical replicates are lower for EdgeSeq (6.9%) and RNA-seq (8.2%) than for FirePlex (22.4%); nCounter replicates are not performed. Receiver operating characteristic analysis for distinguishing present versus absent miRNAs shows small RNA-seq (area under curve 0.99) is superior to EdgeSeq (0.97), nCounter (0.94), and FirePlex (0.81). Expected differences in expression of placenta-associated miRNAs in plasma from pregnant and non-pregnant women are observed with RNA-seq and EdgeSeq, but not FirePlex or nCounter. These results indicate that differences in performance among miRNA profiling platforms impact ability to detect biological differences among samples and thus their relative utility for research and clinical use

Avaliação do comportamento de sementes de algodoeiro (Gossypium hirsutum L.) durante o armazenamento.

Foi avaliado o comportamento das sementes, através de testes de germinação e de vigor