964 research outputs found

    Optimizing ISOCAM data processing using spatial redundancy

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    We present new data processing techniques that allow to correct the main instrumental effects that degrade the images obtained by ISOCAM, the camera on board the Infrared Space Observatory (ISO). Our techniques take advantage of the fact that a position on the sky has been observed by several pixels at different times. We use this information (1) to correct the long term variation of the detector response, (2) to correct memory effects after glitches and point sources, and (3) to refine the deglitching process. Our new method allows the detection of faint extended emission with contrast smaller than 1% of the zodiacal background. The data reduction corrects instrumental effects to the point where the noise in the final map is dominated by the readout and the photon noises. All raster ISOCAM observations can benefit from the data processing described here. These techniques could also be applied to other raster type observations (e.g. ISOPHOT or IRAC on SIRTF).Comment: 13 pages, 10 figures, to be published in Astronomy and Astrophysics Supplement Serie

    GHIGLS: HI mapping at intermediate Galactic latitude using the Green Bank Telescope

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    This paper introduces the data cubes from GHIGLS, deep Green Bank Telescope surveys of the 21-cm line emission of HI in 37 targeted fields at intermediate Galactic latitude. The GHIGLS fields together cover over 1000 square degrees at 9.55' spatial resolution. The HI spectra have an effective velocity resolution about 1.0 km/s and cover at least -450 < v < +250 km/s. GHIGLS highlights that even at intermediate Galactic latitude the interstellar medium is very complex. Spatial structure of the HI is quantified through power spectra of maps of the column density, NHI. For our featured representative field, centered on the North Ecliptic Pole, the scaling exponents in power-law representations of the power spectra of NHI maps for low, intermediate, and high velocity gas components (LVC, IVC, and HVC) are -2.86 +/- 0.04, -2.69 +/- 0.04, and -2.59 +/- 0.07, respectively. After Gaussian decomposition of the line profiles, NHI maps were also made corresponding to the narrow-line and broad-line components in the LVC range; for the narrow-line map the exponent is -1.9 +/- 0.1, reflecting more small scale structure in the cold neutral medium (CNM). There is evidence that filamentary structure in the HI CNM is oriented parallel to the Galactic magnetic field. The power spectrum analysis also offers insight into the various contributions to uncertainty in the data. The effect of 21-cm line opacity on the GHIGLS NHI maps is estimated.Comment: Accepted for publication in The Astrophysical Journal, 2015 July 16. 32 pages, 21 figures (Fig. 10 new). Minor revisions from review, particularly Section 8 and Appendix C; results unchanged. Additional surveys added and made available; new Appendix B. Added descriptions of available FITS files and links to four illustrative movies on enhanced GHIGLS archive (www.cita.utoronto.ca/GHIGLS/

    Analysis of Diffusion of Ras2 in Saccharomyces cerevisiae Using Fluorescence Recovery after Photobleaching

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    Binding, lateral diffusion and exchange are fundamental dynamic processes involved in protein association with cellular membranes. In this study, we developed numerical simulations of lateral diffusion and exchange of fluorophores in membranes with arbitrary bleach geometry and exchange of the membrane localized fluorophore with the cytosol during Fluorescence Recovery after Photobleaching (FRAP) experiments. The model simulations were used to design FRAP experiments with varying bleach region sizes on plasma-membrane localized wild type GFP-Ras2 with a dual lipid anchor and mutant GFP-Ras2C318S with a single lipid anchor in live yeast cells to investigate diffusional mobility and the presence of any exchange processes operating in the time scale of our experiments. Model parameters estimated using data from FRAP experiments with a 1 micron x 1 micron bleach region-of-interest (ROI) and a 0.5 micron x 0.5 micron bleach ROI showed that GFP-Ras2, single or dual lipid modified, diffuses as single species with no evidence of exchange with a cytoplasmic pool. This is the first report of Ras2 mobility in yeast plasma membrane. The methods developed in this study are generally applicable for studying diffusion and exchange of membrane associated fluorophores using FRAP on commercial confocal laser scanning microscopes.Comment: Accepted for publication in Physical Biology (2010). 28 pages, 7 figures, 3 table

    Consistent Modeling of Rotational Nonequilibrium in a Hybrid Particle-Continuum Method

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    Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106474/1/AIAA2013-3145.pd

    Explanatory Supplement of the ISOGAL-DENIS Point Source Catalogue

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    We present version 1.0 of the ISOGAL-DENIS Point Source Catalogue (PSC), containing more than 100,000 point sources detected at 7 and/or 15 micron in the ISOGAL survey of the inner Galaxy with the ISOCAM instrument on board the Infrared Space Observatory (ISO). These sources are cross-identified, wherever possible, with near-infrared (0.8-2.2 micron) data from the DENIS survey. The overall surface covered by the ISOGAL survey is about 16 square degrees, mostly (95%) distributed near the Galactic plane (|b| < 1 deg), where the source extraction can become confusion limited and perturbed by the high background emission. Therefore, special care has been taken aimed at limiting the photometric error to ~0.2 magnitude down to a sensitivity limit of typically 10 mJy. The present paper gives a complete description of the entries and the information which can be found in this catalogue, as well as a detailed discussion of the data processing and the quality checks which have been completed. The catalogue is available via the VizieR Service at the Centre de Donn\'ees Astronomiques de Strasbourg (CDS, http://vizier.u-strasbg.fr/viz-bin/VizieR/) and also via the server at the Institut d'Astrophysique de Paris (http://www-isogal.iap.fr/). A more complete version of this paper, including a detailed description of the data processing, is available in electronic form through the ADS service.Comment: 21 pages, 7 figures. A&A in press. Full length version with 32 figures and detailed description of the data processing is available here: http://www-isogal.iap.fr/Publications/ExplSupplFull.ps.g

    On the use of fractional Brownian motion simulations to determine the 3D statistical properties of interstellar gas

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    Based on fractional Brownian motion (fBm) simulations of 3D gas density and velocity fields, we present a study of the statistical properties of spectro-imagery observations (channel maps, integrated emission, and line centroid velocity) in the case of an optically thin medium at various temperatures. The power spectral index gamma_W of the integrated emission is identified with that of the 3D density field (gamma_n) provided the medium's depth is at least of the order of the largest transverse scale in the image, and the power spectrum of the centroid velocity map is found to have the same index gamma_C as that of the velocity field (gamma_v). Further tests with non-fBm density and velocity fields show that this last result holds, and is not modified either by the effects of density-velocity correlations. A comparison is made with the theoretical predictions of Lazarian & Pogosyan (2000).Comment: 28 pages, 14 figures, accepted for publication in ApJ. For preprint with higher-resolution figures, see http://www.cita.utoronto.ca/~mamd/miville_fbm2003.pd

    Excitation lines and the breakdown of Stokes-Einstein relations in supercooled liquids

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    By applying the concept of dynamical facilitation and analyzing the excitation lines that result from this facilitation, we investigate the origin of decoupling of transport coefficients in supercooled liquids. We illustrate our approach with two classes of models. One depicts diffusion in a strong glass former, and the other in a fragile glass former. At low temperatures, both models exhibit violation of the Stokes-Einstein relation, Dτ1D\sim\tau^{-1}, where DD is the self diffusion constant and τ\tau is the structural relaxation time. In the strong case, the violation is sensitive to dimensionality dd, going as Dτ2/3D\sim\tau^{-2/3} for d=1d=1, and as Dτ0.95D\sim \tau^{-0.95} for d=3d=3. In the fragile case, however, we argue that dimensionality dependence is weak, and show that for d=1d=1, Dτ0.73D \sim \tau^{-0.73}. This scaling for the fragile case compares favorably with the results of a recent experimental study for a three-dimensional fragile glass former.Comment: 7 pages, 7 figures, submitted to Phys. Rev.

    Rotational Correlation Functions of Single Molecules

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    Single molecule rotational correlation functions are analyzed for several reorientation geometries. Even for the simplest model of isotropic rotational diffusion our findings predict non-exponential correlation functions to be observed by polarization sensitive single molecule fluorescence microscopy. This may have a deep impact on interpreting the results of molecular reorientation measurements in heterogeneous environments.Comment: 5 pages, 4 figure

    Structure and Colors of Diffuse Emission in the Spitzer Galactic First Look Survey

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    We investigate the density structure of the interstellar medium using new high-resolution maps of the 8 micron, 24 micron, and 70 micron surface brightness towards a molecular cloud in the Gum Nebula, made as part of the Spitzer Space Telescope Galactic First Look Survey. The maps are correlated with 100 micron images measured with IRAS. At 24 and 70 micron, the spatial power spectrum of surface brightness follows a power law with spectral index -3.5. At 24 micron, the power law behavior is remarkably consistent from the 0.2 degree size of our maps down to the 5 arcsecond spatial resolution. Thus, the structure of the 24 micron emission is self-similar even at milliparsec scales. The combined power spectrum produced from Spitzer 24 micron and IRAS 25 micron images is consistent with a change in the power law exponent from -2.6 to -3.5. The decrease may be due to the transition from a two-dimensional to three-dimensional structure. Under this hypothesis, we estimate the thickness of the emitting medium to be 0.3 pc.Comment: 13 Pages, 3 Figures, to be published in Astrophysical Journal Supplement Series (Spitzer Special Issue), volume 154. Uses aastex v5.
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