621 research outputs found
Developmental Phases of Individual Mouse Preimplantation Embryos Characterized by Lipid Signatures using Desorption Electrospray Ionization Mass Spectrometry
Multiple Reaction Monitoring Profiling (MRM-Profiling) of Lipids To Distinguish Strain-Level Differences in Microbial Resistance in Escherichia coli
The worldwide increase in antimicrobial resistance is due to antibiotic overuse in agriculture and overprescription in medicine. For appropriate and timely patient support, faster diagnosis of antimicrobial resistance is required. Current methods for bacterial identification rely on genomics and proteomics and use comparisons with databases of known strains, but the diagnostic value of metabolites and lipids has not been explored significantly. Standard mass spectrometry/chromatography methods involve multiple dilutions during sample preparation and separation. To increase the amount of chemical information acquired and the speed of analysis of lipids, multiple reaction monitoring profiling (MRM-Profiling) has been applied. The MRM-Profiling workflow includes a discovery stage and a screening stage. The discovery stage employs precursor (PREC) ion and neutral loss (NL) scans to screen representative pooled samples for functional groups associated with particular lipid classes. The information from the first stage is organized in precursor/product ion pairs, or MRMs, and the screening stage rapidly interrogates individual samples for these MRMs. In this study, we performed MRM-Profiling of lipid extracts from four different strains of Escherichia coli cultured with amoxicillin or amoxicillin/clavulanate, a β-lactam and β-lactamase inhibitor, respectively. t tests, analysis of variance and receiver operating characteristic (ROC) curves were used to determine the significance of each MRM. Principal component analysis was applied to distinguish different strains cultured under conditions that allowed or disallowed development of bacterial resistance. The results demonstrate that MRM-Profiling distinguishes the lipid profiles of resistant and nonresistant E. coli strains
On the gravitational production of superheavy dark matter
The dark matter in the universe can be in the form of a superheavy matter
species (WIMPZILLA). Several mechanisms have been proposed for the production
of WIMPZILLA particles during or immediately following the inflationary epoch.
Perhaps the most attractive mechanism is through gravitational particle
production, where particles are produced simply as a result of the expansion of
the universe. In this paper we present a detailed numerical calculation of
WIMPZILLA gravitational production in hybrid-inflation models and
natural-inflation models. Generalizing these findings, we also explore the
dependence of the gravitational production mechanism on various models of
inflation. We show that superheavy dark matter production seems to be robust,
with Omega_X h^2 ~ (M_X / (10^11 GeV))^2 (T_RH / (10^9 GeV)), so long as M_X <
H_I, where M_X is the WIMPZILLA mass, T_RH is the reheat temperature, and H_I
is the expansion rate of the universe during inflation.Comment: 26 pages, 7 figures; LaTeX; submitted to Physical Review D; minor
typographical error correcte
Direct Plant Tissue Analysis and Imprint Imaging by Desorption Electrospray Ionization Mass Spectrometry
A comprehensive evaluation of the kinetic method applied in the determination of the proton affinity of the nucleic acid molecules
Isomeric discrimination and quantification of thyroid hormones, T3 and rT3, by the single ratio kinetic method using electrospray ionization mass spectrometry
Whiteness, Blackness and Settlement: Leisure and the Integration of New Migrants
At times of economic uncertainty the position of new migrants is subject to ever closer scrutiny. While the main focus of attention tends to be on the world of employment the research on which this paper is based started from the proposition that leisure and sport spaces can support processes of social inclusion yet may also serve to exclude certain groups. As such, these spaces may be seen as contested and racialised places that shape behaviour. The paper draws on interviews with White migrants from Poland and Black migrants from Africa to examine the normalising of whiteness. We use this paper not just to explore how leisure and sport spaces are encoded by new migrants, but how struggles over those spaces and the use of social and cultural capital are racialised
Distinction and quantitation of sugar isomers in ternary mixtures using the kinetic method
Alkali chloride cluster ion dissociation examined by the kinetic method: Heterolytic bond dissociation energies, effective temperatures, and entropic effects
Reform, Justice, and Sovereignty: A Food Systems Agenda for Environmental Communication
Food ecologies and economies are vital to the survival of communities, non-human species, and our planet. While environmental communication scholars have legitimated food as a topic of inquiry, the entangled ecological, cultural, economic, racial, colonial, and alimentary relations that sustain food systems demand greater attention. In this essay, we review literature within and beyond environmental communication, charting the landscape of critical food work in our field. We then illustrate how environmental justice commitments can invigorate interdisciplinary food systems-focused communication scholarship articulating issues of, and critical responses to, injustice and inequity across the food chain. We stake an agenda for food systems communication by mapping three orientations—food system reform, justice, and sovereignty—that can assist in our critical engagements with and interventions into the food system. Ultimately, we entreat environmental communication scholars to attend to the bends, textures, and confluences of these orientations so that we may deepen our future food-related inquiries
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