141 research outputs found
Heterologous Expression of ATG8c from Soybean Confers Tolerance to Nitrogen Deficiency and Increases Yield in Arabidopsis
Nitrogen is an essential element for plant growth and yield. Improving Nitrogen Use Efficiency (NUE) of crops could potentially reduce the application of chemical fertilizer and alleviate environmental damage. To identify new NUE genes is therefore an important task in molecular breeding. Macroautophagy (autophagy) is an intracellular process in which damaged or obsolete cytoplasmic components are encapsulated in double membraned vesicles termed autophagosomes, then delivered to the vacuole for degradation and nutrient recycling. One of the core components of autophagosome formation, ATG8, has been shown to directly mediate autophagosome expansion, and the transcript of which is highly inducible upon starvation. Therefore, we postulated that certain homologs of Saccharomyces cerevisiae ATG8 (ScATG8) from crop species could have potential for NUE crop breeding. A soybean (Glycine max, cv. Zhonghuang-13) ATG8, GmATG8c, was selected from the 11 family members based on transcript analysis upon nitrogen deprivation. GmATG8c could partially complement the yeast atg8 mutant. Constitutive expression of GmATG8c in soybean callus cells not only enhanced nitrogen starvation tolerance of the cells but accelerated the growth of the calli. Transgenic Arabidopsis over-expressing GmATG8c performed better under extended nitrogen and carbon starvation conditions. Meanwhile, under optimum growth conditions, the transgenic plants grew faster, bolted earlier, produced larger primary and axillary inflorescences, eventually produced more seeds than the wild-type. In average, the yield was improved by 12.9%. We conclude that GmATG8c may serve as an excellent candidate for breeding crops with enhanced NUE and better yield
Genetic control of yield and yield components in winter oilseed rape (Brassica napus L.) grown under nitrogen limitation
Sur une nouvelle méthode de dosage de l'azote dans les substances organiques
J. KjeldahlSA. aus Résumé du compte-rendu des travaux du laboratoire de Carlsberg, 2me volume , première livraison., Copenhague 188
Ãœber die Anwendung der Mikro-Kjeldahl-Pregl-Methode zur Bestimmung des Gesamt-Stickstoffs in Hefe
Determination of protein and total nitrogen using FP-228 in comparison with other methods
Vergleichende Untersuchungen �ber die Bestimmung des Stickstoffgehaltes nach den Methoden von Kjeldahl und Dumas
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