Block-Conditional Missing at Random Models for Missing Data

Two major ideas in the analysis of missing data are (a) the EM algorithm [Dempster, Laird and Rubin, J. Roy. Statist. Soc. Ser. B 39 (1977) 1--38] for maximum likelihood (ML) estimation, and (b) the formulation of models for the joint distribution of the data ${Z}$ and missing data indicators ${M}$, and associated "missing at random"; (MAR) condition under which a model for ${M}$ is unnecessary [Rubin, Biometrika 63 (1976) 581--592]. Most previous work has treated ${Z}$ and ${M}$ as single blocks, yielding selection or pattern-mixture models depending on how their joint distribution is factorized. This paper explores "block-sequential"; models that interleave subsets of the variables and their missing data indicators, and then make parameter restrictions based on assumptions in each block. These include models that are not MAR. We examine a subclass of block-sequential models we call block-conditional MAR (BCMAR) models, and an associated block-monotone reduced likelihood strategy that typically yields consistent estimates by selectively discarding some data. Alternatively, full ML estimation can often be achieved via the EM algorithm. We examine in some detail BCMAR models for the case of two multinomially distributed categorical variables, and a two block structure where the first block is categorical and the second block arises from a (possibly multivariate) exponential family distribution.Comment: Published in at http://dx.doi.org/10.1214/10-STS344 the Statistical Science (http://www.imstat.org/sts/) by the Institute of Mathematical Statistics (http://www.imstat.org

A Perspective on the Scientific Registry of Transplant Recipients' Migration to Bayesian Methods

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/112268/1/ajt13354.pd

Safety, feasibility and cost of outpatient radiofrequency catheter ablation of accessory atrioventricular connections

AbstractObjectives. The purpose of this study was to evaluate prospectively the safety, feasibility and cost of performing radiofrequency catheter ablation of accessory atrioventricular (AV) connections on an outpatient basis in 137 cases.Background. The efficacy and low complication rate of radiofrequency ablation as performed in the hospital suggested that it might be feasible to perform it on an outpatient basis.Methods. In 100 cases (73%) performed between September 1, 1991 and April 20, 1992, patients met criteria for treatment as outpatients. Reasons for exclusion were age <13 or >70 years (4), anteroseptal location of the accessory AV connection (5 patients), obesity (>30% of ideal body weight) (4 patients) or clinical indication for hospitalization (24 patients). Patients with only venous punctures had a recovery period of 3 h and those with arterial punctures had a recovery period of 6 h. There were 63 men and 32 women (5 patients underwent two ablation procedures >1 month apart), with a mean age ± SD of 36 ± 13 years. The pathway was left-sided in 67 cases and right-sided or posteroseptal in 33.Results. The procedure was successful in 97 of 100 cases, with a mean procedure duration of 99 ± 42 min. In 70 cases the patient was discharged the day of ablation, and in 30 cases the patient required a short (≤18-h) overnight stay because the procedure was completed too late in the day for recovery in the outpatient facility. The mean duration of observation was 4.8 ± 1.5 h for outpatients and 15 ± 1.4 h for patients who underwent overnight hospitalization. At follow-up study, two patients had a clinically significant complication; both had a femoral artery pseudoaneurysm detected ≥1 week after the procedure and both required surgical repair. Thirty consecutive patients (22 outpatients and 8 hospitalized overnight) undergoing catheter ablation after January 1, 1992 were chosen for a cost analysis. The mean cost of the procedure was $10,183 ±$1,082.Conclusions. The majority of patients undergoing radiofrequency catheter ablation of an accessory AV connection can be treated safely on an outpatient basis

Non-Coding RNA Sequencing of Equine Endometrium During Maternal Recognition of Pregnancy.

Maternal recognition of pregnancy (MRP) in the mare is not well defined. In a non-pregnant mare, prostaglandin F2α (PGF) is released on day 14 post-ovulation (PO) to cause luteal regression, resulting in loss of progesterone production. Equine MRP occurs prior to day 14 to halt PGF production. Studies have failed to identify a gene candidate for MRP, so attention has turned to small, non-coding RNAs. The objective of this study was to evaluate small RNA (&lt;200 nucleotides) content in endometrium during MRP. Mares were used in a cross-over design with each having a pregnant and non-mated cycle. Each mare was randomly assigned to collection day 11 or 13 PO (n = 3/day) and endometrial biopsies were obtained. Total RNA was isolated and sequencing libraries were prepared using a small RNA library preparation kit and sequenced on a HiSeq 2000. EquCab3 was used as the reference genome and DESeq2 was used for statistical analysis. On day 11, 419 ncRNAs, representing miRNA, snRNA, snoRNA, scaRNA, and vaultRNA, were different between pregnancy statuses, but none on day 13. Equine endometrial ncRNAs with unknown structure and function were also identified. This study is the first to describe ncRNA transcriptome in equine endometrium. Identifying targets of these ncRNAs could lead to determining MRP

Comparison of Fixed Burst Versus Decremental Burst Pacing for Termination of Ventricular Tachycardia

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72440/1/j.1540-8159.1993.tb01531.x.pd

Functionally Annotating Regulatory Elements in the Equine Genome Using Histone Mark ChIP-Seq.

One of the primary aims of the Functional Annotation of ANimal Genomes (FAANG) initiative is to characterize tissue-specific regulation within animal genomes. To this end, we used chromatin immunoprecipitation followed by sequencing (ChIP-Seq) to map four histone modifications (H3K4me1, H3K4me3, H3K27ac, and H3K27me3) in eight prioritized tissues collected as part of the FAANG equine biobank from two thoroughbred mares. Data were generated according to optimized experimental parameters developed during quality control testing. To ensure that we obtained sufficient ChIP and successful peak-calling, data and peak-calls were assessed using six quality metrics, replicate comparisons, and site-specific evaluations. Tissue specificity was explored by identifying binding motifs within unique active regions, and motifs were further characterized by gene ontology (GO) and protein-protein interaction analyses. The histone marks identified in this study represent some of the first resources for tissue-specific regulation within the equine genome. As such, these publicly available annotation data can be used to advance equine studies investigating health, performance, reproduction, and other traits of economic interest in the horse

Successful ATAC-Seq From Snap-Frozen Equine Tissues

An assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) has become an increasingly popular method to assess genome-wide chromatin accessibility in isolated nuclei from fresh tissues. However, many biobanks contain only snap-frozen tissue samples. While ATAC-seq has been applied to frozen brain tissues in human, its applicability in a wide variety of tissues in horse remains unclear. The Functional Annotation of Animal Genome (FAANG) project is an international collaboration aimed to provide high quality functional annotation of animal genomes. The equine FAANG initiative has generated a biobank of over 80 tissues from two reference female animals and experiments to begin to characterize tissue specificity of genome function for prioritized tissues have been performed. Due to the logistics of tissue collection and storage, extracting nuclei from a large number of tissues for ATAC-seq at the time of collection is not always practical. To assess the feasibility of using stored frozen tissues for ATAC-seq and to provide a guideline for the equine FAANG project, we compared ATAC-seq results from nuclei isolated from frozen tissue to cryopreserved nuclei (CN) isolated at the time of tissue harvest in liver, a highly cellular homogenous tissue, and lamina, a relatively acellular tissue unique to the horse. We identified 20,000– 33,000 accessible chromatin regions in lamina and 22–61,000 in liver, with consistently more peaks identified using CN isolated at time of tissue collection. Our results suggest that frozen tissues are an acceptable substitute when CN are not available. For more challenging tissues such as lamina, nuclei extraction at the time of tissue collection is still preferred for optimal results. Therefore, tissue type and accessibility to intact nuclei should be considered when designing ATAC-seq experiments

PROGRAM & PROCEEDINGS of the 2022 COLLOQUIUM OF THE STONY BROOK UNIVERSITY INTELLECTUAL PROPERTY PARTNERS Female Perspectives on Entrepreneurship and Research How diverse perspectives inspire creativity, drive innovation, and encourage inclusive economic growth

Diverse and varied perspectives are not only central to our institutional mission, but are essential to our society, as we hope to create a more inclusive, more sustainable and ultimately brighter world. Diverse perspectives and collaboration between different institutions, fields and industries must become the norm. This is the program and proceeding of Stony Brook University\u27s colloquium on female perspectives on entrepreneurship and research and how diverse perspectives inspire creativity, drive innovation, and encourage inclusive economic growth. This was a much-needed discussion in January 2022 that\u27s important for the development of entrepreneurship and research worldwide. At Stony Brook University, the entire research community believes that creating an equitable and integrated work environment is an essential part of the success of its innovation and culture. So, this proceeding provides a transcript of the meaningful moderated discussion between 6 expert panelists about female entrepreneurship and research

Female Perspectives on Entrepreneurship and Research How diverse perspectives inspire creativity, drive innovation, and encourage inclusive economic growth

Diverse and varied perspectives are not only central to our institutional mission, but are essential to our society, as we hope to create a more inclusive, more sustainable and ultimately brighter world. Diverse perspectives and collaboration between different institutions, fields and industries must become the norm. This is the program and proceeding of Stony Brook University\u27s colloquium on female perspectives on entrepreneurship and research and how diverse perspectives inspire creativity, drive innovation, and encourage inclusive economic growth. This was a much-needed discussion in January 2022 that\u27s important for the development of entrepreneurship and research worldwide. At Stony Brook University, the entire research community believes that creating an equitable and integrated work environment is an essential part of the success of its innovation and culture. So, this proceeding provides a transcript of the meaningful moderated discussion between 6 expert panelists about female entrepreneurship and research

Female Perspectives on Entrepreneurship and Research How diverse perspectives inspire creativity, drive innovation, and encourage inclusive economic growth

Diverse and varied perspectives are not only central to our institutional mission, but are essential to our society, as we hope to create a more inclusive, more sustainable and ultimately brighter world. Diverse perspectives and collaboration between different institutions, fields and industries must become the norm. This is the program and proceeding of Stony Brook University\u27s colloquium on female perspectives on entrepreneurship and research and how diverse perspectives inspire creativity, drive innovation, and encourage inclusive economic growth. This was a much-needed discussion in January 2022 that\u27s important for the development of entrepreneurship and research worldwide. At Stony Brook University, the entire research community believes that creating an equitable and integrated work environment is an essential part of the success of its innovation and culture. So, this proceeding provides a transcript of the meaningful moderated discussion between 6 expert panelists about female entrepreneurship and research