1,286 research outputs found
The short-lived MATα2 transcriptional regulator is ubiquitinated in vivo
The substrates of ubiquitin-dependent proteolytic pathways include both damaged or otherwise abnormal proteins and undamaged proteins that are naturally short-lived. Few specific examples of the latter class have been identified, however. Previous work has shown that the cell type-specific MAT-alpha-2 repressor of the yeast Saccharomyces cerevisiae is an extremely short-lived protein. We now demonstrate that alpha-2 is conjugated to ubiquitin in vivo. More than one lysine residue of alpha-2 can be joined to ubiquitin, and some of the ubiquitin moieties form a Lys48-linked multiubiquitin chain. Overexpression of degradation-impaired ubiquitin variants was used to show that at least a significant fraction of alpha-2 degradation is dependent on its ubiquitination
Stability of Impurities with Coulomb Potential in Graphene with Homogeneous Magnetic Field
Given a 2-dimensional no-pair Weyl operator with a point nucleus of charge Z,
we show that a homogeneous magnetic field does not lower the critical charge
beyond which it collapses.Comment: J. Math. Phys. (in press
A Conserved 20S Proteasome Assembly Factor Requires a Cterminal HbYX Motif for Proteasomal Precursor Binding
Dedicated chaperones facilitate the assembly of the eukaryotic proteasome, but how they function remains largely unknown. Here we show that a yeast 20S proteasome assembly factor, Pba1–Pba2, requires a previously overlooked C-terminal hydrophobic-tyrosine-X (HbYX) motif for function. HbYX motifs in proteasome activators open the 20S proteasome entry pore, but Pba1–Pba2 instead binds inactive proteasomal precursors. We discovered an archaeal ortholog of this factor, here named PbaA, that also binds preferentially to proteasomal precursors in a HbYX motif–dependent fashion using the same proteasomal α-ring surface pockets as are bound by activators. PbaA and the related PbaB protein can be induced to bind mature 20S proteasomes if the active sites in the central chamber are occupied by inhibitors. Our data are consistent with an allosteric mechanism in which the maturation of the proteasome active sites determines the binding of assembly chaperones, potentially shielding assembly intermediates or misassembled complexes from nonproductive associations until assembly is complete
Spin polarization of the L-gap surface states on Au(111)
The electron spin polarization (ESP) of the L-gap surface states on Au(111)
is investigated theoretically by means of first-principles electronic-structure
and photoemission calculations. The surface states show a large spin-orbit
induced in-plane ESP which is perpendicular to the in-plane wavevector, in
close analogy to a two-dimensional electron gas with Rashba spin-orbit
interaction. The surface corrugation leads to a small ESP component normal to
the surface, being not reported so far. The surface-states ESP can be probed
qualitatively and quantitatively by spin- and angle-resolved photoelectron
spectroscopy, provided that the initial-state ESP is retained in the
photoemission process and not obscured by spin-orbit induced polarization
effects. Relativistic photoemission calculations provide detailed information
on what photoemission set-ups allow to conclude from the photoelectron ESP on
that of the surface states.Comment: 22 pages with 8 figure
Experimental studies of triplet exciton bands of molecular crystals
Methods of studying properties of triplet exciton states of organic crystals are presented with an emphasis on exposing the dimensionality of excitons. Results of isotopic replacement spectra for 1,4-dibromonaphthalene which is a
linear chain, and for halogenated benzenes, which are likely to be linear chains, are presented. Luminescence studies of linear chain exciton systems are shown to yield information about the stationary states of small clusters. Finally some preliminary studies of two-photon spectra of naphthalene excitons are described
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