Alloreactive cytotoxic T lymphocytes generated in the presence of viral- derived peptides show exquisite peptide and MHC specificity

The nature of alloreactivity to MHC molecules has been enigmatic, primarily because of the observation that allogeneic responses are considerably stronger than syngeneic responses. To better determine the specificity potential of allogeneic responses, we have generated alloreactive CTL specific for exogenous, viral-derived peptide ligands. This approach allowed us to critically evaluate both the peptide- and MHC-specificity of these alloreactive T cells. Exploiting the accessibility of the H-2Ld class I molecule for exogenous peptide ligands, alloreactive CTL were generated that are specific for either murine cytomegalovirus (MCMV) or lymphocytic choriomeningitis virus (LCMV) peptides bound by Ld alloantigens. Peptide specificity was initially observed in bulk cultures of alloreactive CTL only when tested on peptide-sensitized T2.Ld target cells that have defective presentation of endogenous peptides. Subsequent cloning of bulk alloreactive CTL lines generated to MCMV yielded CTL clones that had exquisitely specific MCMV peptide recognition requirement. All of the MCMV/Ld alloreactive CTL clones were also exquisitely MHC-specific in that none of the CTL clones lysed targets expressing MCMV/Lq complexes, even though Lq differs from Ld by only six amino acid residues and Lq also binds the MCMV peptide. This observation clearly demonstrates that alloreactive CTL are capable of the same degree of specificity for target cell recognition as are syngeneic CTL in MHC-restricted responses

Confinement effects in a guided-wave interferometer with millimeter-scale arm separation

Guided-wave atom interferometers measure interference effects using atoms held in a confining potential. In one common implementation, the confinement is primarily two-dimensional, and the atoms move along the nearly free dimension under the influence of an off-resonant standing wave laser beam. In this configuration, residual confinement along the nominally free axis can introduce a phase gradient to the atoms that limits the arm separation of the interferometer. We experimentally investigate this effect in detail, and show that it can be alleviated by having the atoms undergo a more symmetric motion in the guide. This can be achieved by either using additional laser pulses or by allowing the atoms to freely oscillate in the potential. Using these techniques, we demonstrate interferometer measurement times up to 72 ms and arm separations up to 0.42 mm with a well controlled phase, or times of 0.91 s and separations of 1.7 mm with an uncontrolled phase.Comment: 14 pages, 6 figure

Design, fabrication, and delivery of a charge injection device as a stellar tracking device

Six 128 x 128 CID imagers fabricated on bulk silicon and with thin polysilicon upper-level electrodes were tested in a star tracking mode. Noise and spectral response were measured as a function of temperature over the range of +25 C to -40 C. Noise at 0 C and below was less than 40 rms carriers/pixel for all devices at an effective noise bandwidth of 150 Hz. Quantum yield for all devices averaged 40% from 0.4 to 1.0 microns with no measurable temperature dependence. Extrapolating from these performance parameters to those of a large (400 x 400) array and accounting for design and processing improvements, indicates that the larger array would show a further improvement in noise performance -- on the order of 25 carriers. A preliminary evaluation of the projected performance of the 400 x 400 array and a representative set of star sensor requirements indicates that the CID has excellent potential as a stellar tracking device

Ejaculate allocation by male sand martins, Riparia riparia

Males of many species allocate sperm to ejaculates strategically in response to variation in the risk and intensity of sperm competition. The notable exception is passerine birds, in which evidence for strategic allocation is absent. Here we report the results of a study testing for strategic ejaculate allocation in a passerine bird, the sand martin (Riparia riparia). Natural ejaculates were collected from males copulating with a model female. Ejaculates transferred in the presence of a rival male contained significantly more sperm than ejaculates transferred in the absence of a rival male. There was no evidence that this difference was due to the confounding effects of the year of ejaculate collection, the identity of the model female, the colony, the stage of season or the period of the day in which ejaculates were collected. A more detailed examination of the ejaculate patterns of individual males, achieved by the DNA profiling of ejaculates, provided additional evidence for strategic allocation of sperm

Measurement of the ac Stark shift with a guided matter-wave interferometer

We demonstrate the effectiveness of a guided-wave Bose-Einstein condensate interferometer for practical measurements. Taking advantage of the large arm separations obtainable in our interferometer, the energy levels of the 87Rb atoms in one arm of the interferometer are shifted by a calibrated laser beam. The resulting phase shifts are used to determine the ac polarizability at a range of frequencies near and at the atomic resonance. The measured values are in good agreement with theoretical expectations. However, we observe a broadening of the transition near the resonance, an indication of collective light scattering effects. This nonlinearity may prove useful for the production and control of squeezed quantum states.Comment: 5 pages, three figure

Microwave Transport in Metallic Single-Walled Carbon Nanotubes

The dynamical conductance of electrically contacted single-walled carbon nanotubes is measured from dc to 10 GHz as a function of source-drain voltage in both the low-field and high-field limits. The ac conductance of the nanotube itself is found to be equal to the dc conductance over the frequency range studied for tubes in both the ballistic and diffusive limit. This clearly demonstrates that nanotubes can carry high-frequency currents at least as well as dc currents over a wide range of operating conditions. Although a detailed theoretical explanation is still lacking, we present a phenomenological model of the ac impedance of a carbon nanotube in the presence of scattering that is consistent with these results.Comment: Added reference

Enhanced dielectrophoresis of nanocolloids by dimer formation

We investigate the dielectrophoretic motion of charge-neutral, polarizable nanocolloids through molecular dynamics simulations. Comparison to analytical results derived for continuum systems shows that the discrete charge distributions on the nanocolloids have a significant impact on their coupling to the external field. Aggregation of nanocolloids leads to enhanced dielectrophoretic transport, provided that increase in the dipole moment upon aggregation can overcome the related increase in friction. The dimer orientation and the exact structure of the nanocolloid charge distribution are shown to be important in the enhanced transport

Energy-momentum conservation in pre-metric electrodynamics with magnetic charges

A necessary and sufficient condition for energy-momentum conservation is proved within a topological, pre-metric approach to classical electrodynamics including magnetic as well as electric charges. The extended Lorentz force, consisting of mutual actions by F=(E, B) on the electric current and G=(H, D) on the magnetic current, can be derived from an energy-momentum "potential" if and only if the constitutive relation G=G(F) satisfies a certain vanishing condition. The electric-magnetic reciprocity introduced by Hehl and Obukhov is seen to define a complex structure on the tensor product of 2-form pairs (F,G) which is independent of but consistent with the Hodge star operator defined by any Lorentzian metric. Contrary to a recent claim in the literature, it does not define a complex structure on the space of 2-forms itself.Comment: 8 pages, 1 fugur