Impact of propulsion system R and D on electric vehicle performance and cost

The efficiency, weight, and manufacturing cost of the propulsion subsystem (motor, motor controller, transmission, and differential, but excluding the battery) are major factors in the purchase price and cost of ownership of a traffic-compatible electric vehicle. The relative impact of each was studied, and the conclusions reached are that propulsion system technology advances can result in a major reduction of the sticker price of an electric vehicle and a smaller, but significant, reduction in overall cost of ownership

Very Light Axigluons and the Top Asymmetry

We show that very light (50 - 90 GeV) axigluons with flavor-universal couplings of order g_{s}/3 may explain the anomalous top forward-backward asymmetry reported by both CDF and D0 collaborations. The model is naturally consistent with the observed t \bar t invariant mass distribution and evades bounds from light Higgs searches, LEP event shapes, and hadronic observables at the Z pole. Very light axigluons can appear as resonances in multijet events, but searches require sensitivity to masses below current limits.Comment: 10 pages, 5 figures, references added, discussion of constraints expanded, general conclusions unchange

Weak-triplet, color-octet scalars and the CDF dijet excess

We extend the standard model to include a weak-triplet and color-octet scalar. This `octo-triplet' field consists of three particles, two charged and one neutral, whose masses and renormalizable interactions depend only on two new parameters. The charged octo-triplet decay into a W boson and a gluon is suppressed by a loop factor and an accidental cancellation. Thus, the main decays of the charged octo-triplet may occur through higher-dimensional operators, mediated by a heavy vectorlike fermion, into quark pairs. For an octo-triplet mass below the t\bar{b} threshold, the decay into Wb\bar{b} or Wb\bar{s} through an off-shell top quark has a width comparable to that into c\bar{s} or c\bar{b}. Pair production with one octo-triplet decaying into two jets and the other decaying into a W and two soft b jets may explain the dijet-plus-W excess reported by the CDF Collaboration. Using a few kinematic distributions, we compare two mechanisms of octo-triplet pair production: through an s-channel coloron and through the coupling to gluons. The higher-dimensional operators that allow dijet decays also lead to CP violation in B_s - \bar B_s mixing.Comment: 18 pages. New CDF kinematic distributions using 7.3 fb^{-1} compared to both resonant and gluon-induced pair production of octets. Corrections in Section 3.1. Comment on the D0 Wjj result included in Section 3.3. Implications for LHC expanded in Section 3.

Distinguishing direct versus indirect transcription factor–DNA interactions

Transcriptional regulation is largely enacted by transcription factors (TFs) binding DNA. Large numbers of TF binding motifs have been revealed by ChIP-chip experiments followed by computational DNA motif discovery. However, the success of motif discovery algorithms has been limited when applied to sequences bound in vivo (such as those identified by ChIP-chip) because the observed TF–DNA interactions are not necessarily direct: Some TFs predominantly associate with DNA indirectly through protein partners, while others exhibit both direct and indirect binding. Here, we present the first method for distinguishing between direct and indirect TF–DNA interactions, integrating in vivo TF binding data, in vivo nucleosome occupancy data, and motifs from in vitro protein binding microarray experiments. When applied to yeast ChIP-chip data, our method reveals that only 48% of the data sets can be readily explained by direct binding of the profiled TF, while 16% can be explained by indirect DNA binding. In the remaining 36%, none of the motifs used in our analysis was able to explain the ChIP-chip data, either because the data were too noisy or because the set of motifs was incomplete. As more in vitro TF DNA binding motifs become available, our method could be used to build a complete catalog of direct and indirect TF–DNA interactions. Our method is not restricted to yeast or to ChIP-chip data, but can be applied in any system for which both in vivo binding data and in vitro DNA binding motifs are available.National Science Foundation (U.S.). (CAREER Award 0347801

Aortic Embolism in Cats: Prevalence, Surgical Treatment and Electrocardiography

Aortic embolism (caudal arterial thromboembolism) was diagnosed over a four-year-period in 14 out of 2,000 cats in a hospital clinic population (7/1,000). Including 35 cases reported in the literature, the average age of 50 cats with aortic embolism was 6-8 years (range one to 16 years). Of these, 37 were males and 13 were females. Endocarditis with thrombosis was the most frequently observed cause of aortic embolism, although aortic arteriosclerosis was reported in one cat. The clinical and pathological features of aortic embolism in five cats are described in this report. In electrocardiograms of four of these, arrhythmias or conduction disturbances were recorded. Intact emboli in the aorta and external iliac arteries were removed by abdominal aortic embolectomy in two cats within hours after the onset of posterior paralysis. Death resulted in one case from cardiac complications and in the other by euthanasia at the later date because of probably recurrent aortic embolism. In the other three cases, multiple sections of the aorta with the embolus in situ were examined, but no microscopic changes in the aortic wall were noted. Surgical removal of an aortic embolus is technically and economically feasible and is considered the treatment of choice when treatment is requested within hours after the onset of clinical signs. Although embolectomy can yield a good immediate result; the long range justification for such therapy requires further evaluation, since recurrent embolization may develop

On the theory of Gordan-Noether on homogeneous forms with zero Hessian (Improved version)

We give a detailed proof for Gordan-Noether's results in "Ueber die algebraischen Formen, deren Hesse'sche Determinante identisch verschwindet" published in 1876 in Mathematische Annahlen. C. Lossen has written a paper in a similar direction as the present paper, but did not provide a proof for every result. In our paper, every result is proved. Furthermore, our paper is independent of Lossen's paper and includes a considerable number of new observations. An earlier version of this paper has been printed in Proceedings of the School of Science of Tokai University, Vol.49, Mar. 2014. In this version, a serious error has been corrected and some new results have been added

Survey of variation in human transcription factors reveals prevalent DNA binding changes

Published in final edited form as: Science. 2016 Mar 25; 351(6280): 1450–1454. Published online 2016 Mar 24. doi: 10.1126/science.aad2257Sequencing of exomes and genomes has revealed abundant genetic variation affecting the coding sequences of human transcription factors (TFs), but the consequences of such variation remain largely unexplored. We developed a computational, structure-based approach to evaluate TF variants for their impact on DNA binding activity and used universal protein-binding microarrays to assay sequence-specific DNA binding activity across 41 reference and 117 variant alleles found in individuals of diverse ancestries and families with Mendelian diseases. We found 77 variants in 28 genes that affect DNA binding affinity or specificity and identified thousands of rare alleles likely to alter the DNA binding activity of human sequence-specific TFs. Our results suggest that most individuals have unique repertoires of TF DNA binding activities, which may contribute to phenotypic variation.National Institutes of Health; NHGRI R01 HG003985; P50 HG004233; A*STAR National Science Scholarship; National Science Foundatio

Sequence specificity incompletely defines the genome-wide occupancy of Myc

BACKGROUND: The Myc-Max heterodimer is a transcription factor that regulates expression of a large number of genes. Genome occupancy of Myc-Max is thought to be driven by Enhancer box (E-box) DNA elements, CACGTG or variants, to which the heterodimer binds in vitro. RESULTS: By analyzing ChIP-Seq datasets, we demonstrate that the positions occupied by Myc-Max across the human genome correlate with the RNA polymerase II, Pol II, transcription machinery significantly better than with E-boxes. Metagene analyses show that in promoter regions, Myc is uniformly positioned about 100 bp upstream of essentially all promoter proximal paused polymerases with Max about 15 bp upstream of Myc. We re-evaluate the DNA binding properties of full length Myc-Max proteins. Electrophoretic mobility shift assay results demonstrate Myc-Max heterodimers display significant sequence preference, but have high affinity for any DNA. Quantification of the relative affinities of Myc-Max for all possible 8-mers using universal protein-binding microarray assays shows that sequences surrounding core 6-mers significantly affect binding. Compared to the in vitro sequence preferences, Myc-Max genomic occupancy measured by ChIP-Seq is largely, although not completely, independent of sequence specificity. CONCLUSIONS: We quantified the affinity of Myc-Max to all possible 8-mers and compared this with the sites of Myc binding across the human genome. Our results indicate that the genomic occupancy of Myc cannot be explained by its intrinsic DNA specificity and suggest that the transcription machinery and associated promoter accessibility play a predominant role in Myc recruitment. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13059-014-0482-3) contains supplementary material, which is available to authorized users