Application de la methode de fixation-coloration de Maillet à certains Invertébrés

Maillet’s osmium-zinc iodide technique gave the most interesting results and demonstrated the presence of amyelinated fibers in vertebrates. This method therefore was tried for invertebrates, including the Mollusca, where it proved fully efficient

Endometrium Cell Surface Abnormalities in the Syrian Hamster as a Result of In Utero Exposure to Diethylstilbestrol

Scanning electron microscopy (SEM) was used to observe changes in the hamster endometrium cell surface following in utero pre- and/or postnatal exposure to diethylstilbestrol (DES). Some of the changes in cell surfaces are associated with alterations in cell sizes and shapes (from columnar to cuboidal and/or squamous) and in microvilli and mucous secretion. In all cases, DES treated uteri show mucosal cell surface pleomorphism, apocrine secretion and cystic accumulation of secretory material. Microvillous pleomorphism and peculiar linkages attaching one microvillus to others were investigated. Although the function and nature of such linkages is unclear, their presence seems to be more prominent in the in utero DES treated hamster endometrium. These infrastructures may provide a support for the microvilli distributed on the mucosal cell surfaces, i.e., a morphological compromise between the single microvillous surface and the microridged structures. These interconnections may represent glycocalyx material or remodeling of cell surfaces toward squamous epithelium

Realistic turbulent inflow conditions for estimating the performance of a floating wind turbine

A novel method for generating turbulent inflow boundary conditions for aeroelastic computations is proposed, based on interfacing hybrid hot-wire and particle image velocimetry measurements performed in a wind tunnel to a full-scale load simulation conducted with FAST. This approach is based on the use of proper orthogonal decomposition (POD) to interpolate and extrapolate the experimental data onto the numerical grid. The temporal dynamics of the temporal POD coefficients is driven by the high-frequency hot-wire measurements used as input for a lower-order model built using a multi-time-delay linear stochastic estimation (LSE) approach. Being directly extracted from the data, the generated three-component velocity fields later used as inlet conditions present correct one- and two-point spatial statistics and realistic temporal dynamics. Wind tunnel measurements are performed at a scale of 1:750, using a properly scaled porous disk as a floating wind turbine model. The motions of the platform are imposed by a linear actuator. Between all 6 degrees of freedom (DOFs) possible, the present study focus on the streamwise direction motion of the model (surge motion). The POD analysis of the flow, with or without considering the presence of the surge motion of the model, shows that a few modes are able to capture the characteristics of the most energetic flow structures and the main features of the wind turbine wake, such as its meandering and the influence of the surge motion. The interfacing method is first tested to estimate the performance of a wind turbine in an offshore boundary layer and then those of a wind turbine immersed in the wake of an upstream wind turbine subjected to a sinusoidal surge motion. Results are also compared to those obtained using the standard inflow generation method provided by TurbSim available in FAST.</p

α-Tocopheryl succinate promotes selective cell death induced by vitamin K3 in combination with ascorbate

BACKGROUND: A strategy to reduce the secondary effects of anti-cancer agents is to potentiate the therapeutic effect by their combination. A combination of vitamin K3 (VK3) and ascorbic acid (AA) exhibited an anti-cancer synergistic effect, associated with extracellular production of H2O2 that promoted cell death. METHODS: The redox-silent vitamin E analogue a-tocopheryl succinate (a-TOS) was used in combination with VK3 and AA to evaluate their effect on prostate cancer cells. RESULTS: Prostate cancer cells were sensitive to a-TOS and VK3 treatment, but resistant to AA upto 3.2mM. When combined, a synergistic effect was found for VK3\u2013AA, whereas a-TOS\u2013VK3 and a-TOS\u2013AA combination showed an antagonist and additive effect, respectively. However, sub-lethal doses of AA\u2013VK3 combination combined with a sub-toxic dose of a-TOS showed to induce efficient cell death that resembles autoschizis. Associated with this cell demise, lipid peroxidation, DNA damage, cytoskeleton alteration, lysosomal\u2013mitochondrial perturbation, and release of cytochrome c without caspase activation were observed. Inhibition of lysosomal proteases did not attenuate cell death induced by the combined agents. Furthermore, cell deaths by apoptosis and autoschizis were detected. CONCLUSION: These finding support the emerging idea that synergistic combinations of some agents can overcome toxicity and other side-effects associated with high doses of single drugs creating the opportunity for therapeutically relevant selectivity

The first World Cell Race

Motility is a common property of animal cells. Cell motility is required for embryogenesis [1], tissue morphogenesis [2] and the immune response [3] but is also involved in disease processes, such as metastasis of cancer cells [4]. Analysis of cell migration in native tissue in vivo has yet to be fully explored, but motility can be relatively easily studied in vitro in isolated cells. Recent evidence suggests that cells plated in vitro on thin lines of adhesive proteins printed onto culture dishes can recapitulate many features of in vivo migration on collagen fibers 5, 6. However, even with controlled in vitro measurements, the characteristics of motility are diverse and are dependent on the cell type, origin and external cues. One objective of the first World Cell Race was to perform a large-scale comparison of motility across many different adherent cell types under standardized conditions. To achieve a diverse selection, we enlisted the help of many international laboratories, who submitted cells for analysis. The large-scale analysis, made feasible by this competition-oriented collaboration, demonstrated that higher cell speed correlates with the persistence of movement in the same direction irrespective of cell origin