Live‐cell super‐resolution imaging of actin using LifeAct‐14 with a PAINT‐based approach

We present direct‐LIVE‐PAINT, an easy‐to‐implement approach for the nanoscopic imaging of protein structures in live cells using labeled binding peptides. We demonstrate the feasibility of direct‐LIVE‐PAINT with an actin‐binding peptide fused to EGFP, the location of which can be accurately determined as it transiently binds to actin filaments. We show that direct‐LIVE‐PAINT can be used to image actin structures below the diffraction‐limit of light and have used it to observe the dynamic nature of actin in live cells. We envisage a similar approach could be applied to imaging other proteins within live mammalian cells

Tracing international migration in projections of income and inequality across the Shared Socioeconomic Pathways

The Shared Socioeconomic Pathways (SSPs) represent five narratives of future development used for climate change research. They include quantified projections of socioeconomic variables such as population, income levels, inequalities, and emissions over the twenty-first century. The SSP’s population projections embody explicit, pathway-specific international migration assumptions, which are only implicit in the projections of other variables. In this contribution, we explicitly quantify the effects of international migration on income levels and income inequality across and within countries by comparing the original SSP projections to scenarios of zero migration. Income projections without migration are obtained by removing two effects of migration on income dynamics: changes in population size and remittances sent to origin countries. We base our remittance estimates on migrant stocks derived from bilateral migration flow estimates obtained from a gravity model. We find that, on average, migration tends to make the world richer in all SSP narratives. The nature of migration and remittance corridors is shaped by the specific scenario of future development considered. Depending on the particular SSP narrative and world region considered, the effects of migration on income can be substantial, ranging from −5 to +21% at the continental level. We show that migration tends to decrease income inequality across countries and within country in most destination countries but does not affect within-country inequality in origin countries. This new set of projections is consistent with the interdisciplinary framework of the SSPs, which makes it particularly useful for assessing global climate and sustainable development policy options

An emission pathway classification reflecting the Paris Agreement climate objectives

The 2015 Paris Agreement sets the objectives of global climate ambition as expressed in itslong-term temperature goal and mitigation goal. The scientific community has explored thecharacteristics of greenhouse gas emission reduction pathways in line with the ParisAgreement. However, when categorizing such pathways, the focus has been put on thetemperature outcome and not on emission reduction objectives. Here we propose a pathwayclassification that aims to comprehensively reflect the climate criteria set out in the ParisAgreement. We show how such an approach allows for a fully consistent interpretation of theAgreement. For Paris Agreement compatible pathways, we report net zero CO2 and greenhousegas emissions around 2050 and 2065, respectively. We illustrate how pathway designcriteria not rooted in the Paris Agreement, such as the 2100 temperature level, result inscenario outcomes wherein about 6 - 24% higher deployment (interquartile range) of carbondioxide removal is observed

Secure robust carbon dioxide removal policy through credible certification

Carbon Dioxide Removal (CDR) is a key element of any mitigation strategy aiming to achieve the long-term temperature goal of the Paris Agreement, as well as national net-zero and net-negative greenhouse gas emissions targets. For robust CDR policy, the credibility of certification schemes is essential

Imaging Proteins Sensitive to Direct Fusions Using Transient Peptide–Peptide Interactions

Fluorescence microscopy enables specific visualization of proteins in living cells and has played an important role in our understanding of the protein subcellular location and function. Some proteins, however, show altered localization or function when labeled using direct fusions to fluorescent proteins, making themdifficult to study in live cells. Additionally, the resolution of fluorescence microscopy is limited to ∼200 nm, which is 2 orders of magnitude larger than the size of most proteins. To circumvent these challenges, we previously developed LIVE-PAINT, a live-cell superresolution approach that takes advantage of short interacting peptides to transiently bind a fluorescent protein to the protein-ofinterest. Here, we successfully use LIVE-PAINT to image yeastmembrane proteins that do not tolerate the direct fusion of a fluorescent protein by using peptide tags as short as 5-residues. We also demonstrate that it is possible to resolve multiple proteins at the nanoscale concurrently using orthogonal peptide interaction pairs.KEYWORDS: membrane protein, protein−protein interaction, super-resolution microscopy, live-cell imaging, LIVE-PAINT, yeas

A new scenario logic for the Paris Agreement long-term temperature goal

To understand how global warming can be kept well below 2 degrees Celsius and even 1.5 degrees Celsius, climate policy uses scenarios that describe how society could reduce its greenhouse gas emissions. However, current scenarios have a key weakness: they typically focus on reaching specific climate goals in 2100. This choice may encourage risky pathways that delay action, reach higher-than-acceptable mid-century warming, and rely on net removal of carbon dioxide thereafter to undo their initial shortfall in reductions of emissions. Here we draw on insights from physical science to propose a scenario framework that focuses on capping global warming at a specific maximum level with either temperature stabilization or reversal thereafter. The ambition of climate action until carbon neutrality determines peak warming, and can be followed by a variety of long-term states with different sustainability implications. The approach proposed here closely mirrors the intentions of the United Nations Paris Agreement, and makes questions of intergenerational equity into explicit design choices

Policy guidance and pitfalls aligning IPCC scenarios to national land emissions inventories

Taking stock of global progress towards achieving the Paris Agreement requires measuring aggregate national action against modelled mitigation pathways. Because of differences in how land-based carbon removals are defined, scientific sources report higher global carbon emissions than national emissions inventories, a gap which will evolve in the future. We establish a first estimate aligning IPCC-assessed pathways with inventories using a climate model to explicitly include indirect carbon removal dynamics on land area reported as managed for by countries. After alignment, we find that key global mitigation benchmarks can appear more ambitious when considering this extra land sink, though changes vary amongst world regions and temperature outcomes. Our results highlight the need to enhance communication between scientific and policy communities to enable more robust alignment in the future