An improved stress corrosion test medium for aluminum alloys

A laboratory test method that is only mildly corrosive to aluminum and discriminating for use in classifying the stress corrosion cracking resistance of aluminum alloys is presented along with the method used in evaluating the media selected for testing. The proposed medium is easier to prepare and less expensive than substitute ocean water

Peroxisome Proliferator-activated receptor alpha gene variation influences age of onset and progression of type 2 diabetes

Dysregulation of fatty acid metabolism is important in the pathogenesis of type 2 diabetes. Peroxisome proliferator-activated receptor (PPAR) is a master regulator of fatty acid catabolism, and PPAR activators delay the onset of type 2 diabetes. We examined association between three PPAR gene polymorphisms (an AC variant in intron 1, the L162V variant, and the intron 7 GC variant) and age at diagnosis of type 2 diabetes in 912 Caucasian type 2 diabetic subjects. Individually, PPAR gene variants did not influence age at diagnosis, but in combination, the rare alleles of both the intron 1 AC (P < 0.001) and intron 7 GC (P = 0.025) variants synergistically lowered age at diagnosis (interaction P < 0.001). Overall, the PPAR haplotype signficantly influenced age at diagnosis (P = 0.027), with the C-L-C and C-V-C haplotypes (intron 1-L162V-intron 7) accelerating onset of diabetes by 5.9 (P = 0.02) and 10 (P = 0.03) years, respectively, as compared with the common A-L-G haplotype, and was associated with an odds ratio for early-onset diabetes (age at diagnosis 45 years) of 3.75 (95% CI 1.65–8.56, P = 0.002). Intron 1 C-allele carriers also progressed more rapidly to insulin monotherapy (AA 9.4 ± 1.5 and AC + CC 5.3 ± 1.1 years, P = 0.002). These data indicate that PPAR gene variation influences the onset and progression of type 2 diabetes

Empirical model for quasi direct current interruption with a convoluted arc

This contribution considers various aspects of a quasi direct current, convoluted arc produced by a magnetic field (B-field) connected in parallel with an RLC circuit that have not been considered in combination. These aspects are the arc current limitation due to the arc convolution, changes in arc resistance due to the B-field and material ablation, and the relative significance of the RLC circuit in producing an artificial current zero. As a result, it has been possible to produce an empirical equation for predicting the current interruption capability in terms of the B-field magnitude and RLC components

A Novel Approach to Power Circuit Breaker Design for Replacement of SF6

This contribution explores the role of PTFE ablation in enhancing current interruption for various background gases in high voltage circuit breakers. An assessment of the current interruption capability has been made in terms of the arcing duration and the contact gap length at which critical arc extinction is achieved. These observations are supported by measurements of the magnitude of extinction and re-ignition voltage peaks. Most previous and other current experimental work on gas filled circuit breaker design follows conventional wisdom in investigating arcing behaviours at elevated gas pressures (usually up to 6 bar). But in this work we concentrate on the effects of using low gas pressures (less than 1 bar) in the presence of a close-fitting shield of ablatant polymer material (PTFE) that surrounds the electrode assembly of an experimental high power circuit breaker. We demonstrate that for several different gases, arc extinction capability compares well under these conditions with SF6, suggesting that SF6 could be replaced entirely in this novel system by more environmentally friendly gases. Moreover, the critical contact gap lengths at extinction are only slightly greater than when using SF6 at 6 bar. Weight loss measurements from the ablatant shield suggest that a chemical puffer action is the most likely mechanism for achieving the observed arc extinctions in this system

Human paraoxonase gene cluster polymorphisms as predictors of coronary heart disease risk in the prospective Northwick Park Heart Study II

AbstractThe anti-atherogenic effect of HDL has been suggested to be partly due to the action of HDL-associated paraoxonase (PON). Three distinct enzymes have been identified, encoded by PON1, PON2 and PON3, clustered on chromosome 7q21–q22. Two cSNPs in PON1 (L55M and Q192R) and one in PON2 (S311C) have been implicated as independent risk factors for coronary heart disease (CHD) in some, but not all, studies. A PON3 SNP (A99A) was identified and the effect of these four PON SNPs on HDL levels and CHD risk was examined in the prospective Northwick Park Heart Study II (NPHSII). Genotype frequencies did not differ between cases and controls but the CHD risk associated with smoking was significantly modified by PON1 L55M genotype. Compared to LL non-smokers, LL smokers had a hazard ratio (HR) of 1.30 (95% CI 0.81–2.06) while M-allele carriers had a HR of 1.76 (1.17–2.67). When genotypes were analysed in combination, men with the genotype PON1 55 LM/MM+PON2 311 CC, had HR of 3.54 (1.81–6.93) compared to PON1 LL+PON2 SS/SC men (interaction P=0.004). These effects were independent of classical risk factors. These data demonstrate the importance of stratifying by environmental factors and the use of multiple SNPs for genetic analysis

Global analysis reveals the complexity of the human glomerular extracellular matrix.

The glomerulus contains unique cellular and extracellular matrix (ECM) components, which are required for intact barrier function. Studies of the cellular components have helped to build understanding of glomerular disease; however, the full composition and regulation of glomerular ECM remains poorly understood. We used mass spectrometry-based proteomics of enriched ECM extracts for a global analysis of human glomerular ECM in vivo and identified a tissue-specific proteome of 144 structural and regulatory ECM proteins. This catalog includes all previously identified glomerular components plus many new and abundant components. Relative protein quantification showed a dominance of collagen IV, collagen I, and laminin isoforms in the glomerular ECM together with abundant collagen VI and TINAGL1. Protein network analysis enabled the creation of a glomerular ECM interactome, which revealed a core of highly connected structural components. More than one half of the glomerular ECM proteome was validated using colocalization studies and data from the Human Protein Atlas. This study yields the greatest number of ECM proteins relative to previous investigations of whole glomerular extracts, highlighting the importance of sample enrichment. It also shows that the composition of glomerular ECM is far more complex than previously appreciated and suggests that many more ECM components may contribute to glomerular development and disease processes. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD000456

Short communication: a survey of grass-clover ley management and creation of a near infra-red reflectance spectroscopy equation to predict clover concentration

The purpose of the present study was, firstly, to examine current practice for the agronomy of grass-clover mixed swards used for silage-making in the UK, and secondly, to develop and validate a Near Infra-Red Reflectance Spectroscopy (NIRS) equation capable of predicting clover concentration (CC) in undried and unmilled grass-clover silage samples. A calibration set of 94 grass-clover (white, trifolium repens, and red, trifolium pratense) mixture silage samples were sourced from UK farms and an accompanying questionnaire was used to obtain information on the sward agronomy used to produce each sample. Questionnaire data highlighted that (i) reducing the use of fertiliser inputs (ii) increasing uptake of new varieties, and (iii) increasing the farmer’s ability to measure botanical composition as potential strategies for improving the utilisation of clover in grass swards. Botanical composition was measured by hand separation for each sample and a new NIRS equation was created and assessed using blind validation with an independent set of 30 grass-clover samples. The relative standard error of cross validation (SECV, as a percentage of the measured mean) of the optimised equation produced was 36.8%, and, in an independent validation test, the ratio of standard error of prediction to the standard deviation of the reference data set (RPD) was 1.56. The equation could be improved by increasing accuracy at high CCs but showed promise as a simple tool to assist growers in sward management decisions

Investigating the Effect of Withania somnifera, Selenium and Hydrocortisone on Blood Count and Bronchial Lavage of Experimental Asthmatic BALB/c Mice

The murine asthmatic Balb/c mouse model was used to investigate the effect of W. somnifera L., the  antioxidant selenium, W. somnifera and selenium in combination and hydrocortisone as positive control  on the number of white blood cells in blood smears and bronchial lavage smears as well as the platelet  distribution of asthmatic Balb/c mice. The mice were sensitised, nebulized and treated over a period of  43 days and blood smears were made of each individual animal and bronchial lavage was performed by  injecting 0.3ml of saline into the trachea of the mice, both on the day of termination. Light microscopy  analysis of the bronchial lavage revealed a significant decrease in the number of eosinophils counted in the  asthmatic and the different treatment groups. In the asthmatic group, numerous platelet clumps were found  distributed between white blood cells. Platelets were also found in the other treatment groups but are not  as prevalent as in the asthmatic group. Results from the blood smears showed the same trends, where cell  counts in control and hydrocortisone blood smears were decreased compared to that of the asthma group. It  is concluded that W. somnifera and W. somnifera combined with selenium significantly decreased the white  blood cells in both bronchial lavage as well as blood smears, suggesting that W. somnifera indeed has an  anti-inflammatory potential and it, in combination with an anti-oxidant like selenium, might successfully  be used in the treatment of asthma.

Reformulation initiative for partial replacement of saturated with unsaturated fats in dairy foods attenuates the increase in LDL cholesterol and improves flow-mediated dilatation compared with conventional dairy: the randomized, controlled REplacement of SaturatEd fat in dairy on Total cholesterol (RESET) study

Background Modifying dairy fat composition by increasing the MUFA content is a potential strategy to reduce dietary SFA intake for cardiovascular disease (CVD) prevention in the population. Objectives To determine the effects of consuming SFA-reduced, MUFA-enriched (modified) dairy products, compared with conventional dairy products (control), on the fasting cholesterol profile (primary outcome), endothelial function assessed by flow-mediated dilatation (FMD; key secondary outcome), and other cardiometabolic risk markers. Methods A double-blind, randomized, controlled crossover 12-wk intervention was conducted. Participants with a 1.5-fold higher (moderate) CVD risk than the population mean replaced habitual dairy products with study products (milk, cheese, and butter) to achieve a high-fat, high-dairy isoenergetic daily dietary exchange [38% of total energy intake (%TE) from fat: control (dietary target: 19%TE SFA; 11%TE MUFA) and modified (16%TE SFA; 14%TE MUFA) diet]. Results Fifty-four participants (57.4% men; mean ± SEM age: 52 ± 3 y; BMI: 25.8 ± 0.5 kg/m2) completed the study. The modified diet attenuated the rise in fasting LDL cholesterol observed with the control diet (0.03 ± 0.06 mmol/L and 0.19 ± 0.05 mmol/L, respectively; P = 0.03). Relative to baseline, the %FMD response increased after the modified diet (0.35% ± 0.15%), whereas a decrease was observed after the control diet (−0.51% ± 0.15%; P< 0.0001). In addition, fasting plasma nitrite concentrations increased after the modified diet, yet decreased after the control diet (0.02 ± 0.01 μmol/L and −0.03 ± 0.02 μmol/L, respectively; P = 0.01). Conclusions In adults at moderate CVD risk, consumption of a high-fat diet containing SFA-reduced, MUFA-enriched dairy products for 12 wk showed beneficial effects on fasting LDL cholesterol and endothelial function compared with conventional dairy products. Our findings indicate that fatty acid modification of dairy products may have potential as a public health strategy aimed at CVD risk reduction. This trial was registered at clinicaltrials.gov as NCT02089035