Folding and Unfolding in the Blue Copper Protein Rusticyanin: Role of the Oxidation State

The unfolding process of the blue copper protein rusticyanin has been studied from the structural and the thermodynamic points of view at two pH values (pH 2.5 and 7.0). When Rc unfolds, copper ion remains bound to the polypeptide chain. Nuclear magnetic resonance data suggest that three of the copper ligands in the folded state are bound to the metal ion in the unfolded form, while the other native ligand is detached. These structural changes are reflected in the redox potentials of the protein in both folded and unfolded forms. The affinities of the copper ion in both redox states have been also determined at the two specified pH values. The results indicate that the presence of two histidine ligands in the folded protein can compensate the change in the net charge that the copper ion receives from their ligands, while, in the unfolded protein, charges of aminoacids are completely transferred to the copper ion, altering decisively the relative stability of its two-redox states

Enhancing methane production from the invasive macroalga Rugulopteryx okamurae through anaerobic co-digestion with olive mill solid waste: process performance and kinetic analysis

The biomass valorisation of the invasive brown alga Rugulopteryx okamurae (Dictyotales, Phaeophyceae) is key to curbing the expansion of this invasive macroalga which is generating tonnes of biomass on southern Spain beaches. As a feasible alternative for the biomass management, anaerobic co-digestion is proposed in this study. Although the anaerobic digestion of macroalgae barely produced 177 mL of CH4 g−1 VS, the co-digestion with a C-rich substrate, such as the olive mill solid waste (OMSW, the main waste derived from the two-phase olive oil manufacturing process), improved the anaerobic digestion process. The mixture improved not only the methane yield, but also its biodegradability. The highest biodegradability was found in the mixture 1 R. okamurae—1 OMSW, which improved the biodegradability of the macroalgae by 12.9% and 38.1% for the OMSW. The highest methane yield was observed for the mixture 1 R. okamurae—3 OMSW, improving the methane production of macroalgae alone by 157% and the OMSW methane production by 8.6%. Two mathematical models were used to fit the experimental data of methane production time with the aim of assessing the processes and obtaining the kinetic constants of the anaerobic co-digestion of different combination of R. okamurae and OMSW and both substrates independently. First-order kinetic and the transference function models allowed for appropriately fitting the experimental results of methane production with digestion time. The specific rate constant, k (first-order model) for the mixture 1 R. okamurae- 1.5 OMSW, was 5.1 and 1.3 times higher than that obtained for the mono-digestion of single OMSW and the macroalga, respectively. In the same way, the transference function model revealed that the maximum methane production rate (Rmax) was also found for the mixture 1 R. okamurae—1.5 OMSW (30.4 mL CH4 g−1 VS day−1), which was 1.6 and 2.2 times higher than the corresponding to the mono-digestions of the single OMSW and sole R. okamurae (18.9 and 13.6 mL CH4 g−1 VS day−1), respectively.Ministerio de Ciencia e Innovación PID2020-114975RB-10

Study of hard double-parton scattering in four-jet events in pp collisions at √s = 7 TeV with the ATLAS experiment

Journal of High Energy Physics 2016.11 (2016): 110 reproduced by permission of Scuola Internazionale Superiore di Studi Avanzati (SISSA)Artículo escrito por muchos autores, sólo se referencian el que aparece en primer lugar, el nombre del grupo de colaboración y los autores que firman como pertenecientes a la UAMInclusive four-jet events produced in proton-proton collisions at a centre-ofmass energy of √ s = 7 TeV are analysed for the presence of hard double-parton scattering using data corresponding to an integrated luminosity of 37.3 pb−1 , collected with the ATLAS detector at the LHC. The contribution of hard double-parton scattering to the production of four-jet events is extracted using an artificial neural network, assuming that hard double-parton scattering can be approximated by an uncorrelated overlaying of dijet events. For events containing at least four jets with transverse momentum pT ≥ 20 GeV and pseudorapidity |η| ≤ 4.4, and at least one having pT ≥ 42.5 GeV, the contribution of hard double-parton scattering is estimated to be fDPS = 0.092 +0.005 −0.011 (stat.) +0.033 −0.037 (syst.). After combining this measurement with those of the inclusive dijet and four-jet cross-sections in the appropriate phase space regions, the effective cross-section, σeff, was determined to be σeff = 14.9 +1.2 −1.0 (stat.) +5.1 −3.8 (syst.) mb. This result is consistent within the quoted uncertainties with previous measurements of σeff, performed at centre-of-mass energies between 63 GeV and 8 TeV using various final states, and it corresponds to 21+7 −6% of the total inelastic cross-section measured at √ s = 7 TeV. The distributions of the observables sensitive to the contribution of hard double-parton scattering, corrected for detector effects, are also providedWe acknowledge the support of ANPCyT, Argentina; YerPhI, Armenia; ARC, Australia; BMWFW and FWF, Austria; ANAS, Azerbaijan; SSTC, Belarus; CNPq and FAPESP, Brazil; NSERC, NRC and CFI, Canada; CERN; CONICYT, Chile; CAS, MOST and NSFC, China; COLCIENCIAS, Colombia; MSMT CR, MPO CR and VSC CR, Czech Republic; DNRF and DNSRC, Denmark; IN2P3-CNRS, CEA-DSM/IRFU, France; GNSF, Georgia; BMBF, HGF, and MPG, Germany; GSRT, Greece; RGC, Hong Kong SAR, China; ISF, I-CORE and Benoziyo Center, Israel; INFN, Italy; MEXT and JSPS, Japan; CNRST, Morocco; FOM and NWO, Netherlands; RCN, Norway; MNiSW and NCN, Poland; FCT, Portugal; MNE/IFA, Romania; MES of Russia and NRC KI, Russian Federation; JINR; MESTD, Serbia; MSSR, Slovakia; ARRS and MIZS, Slovenia; DST/NRF, South Africa; MINECO, Spain; SRC and Wallenberg Foundation, Sweden; SERI, SNSF and Cantons of Bern and Geneva, Switzerland; MOST, Taiwan; TAEK, Turkey; STFC, United Kingdom; DOE and NSF, United States of America. In addition, individual groups and members have received support from BCKDF, the Canada Council, CANARIE, CRC, Compute Canada, FQRNT, and the Ontario Innovation Trust, Canada; EPLANET, ERC, FP7, Horizon 2020 and Marie Sklodowska- Curie Actions, European Union; Investissements d’Avenir Labex and Idex, ANR, Région Auvergne and Fondation Partager le Savoir, France; DFG and AvH Foundation, Germany; Herakleitos, Thales and Aristeia programmes cofinanced by EU-ESF and the Greek NSRF; BSF, GIF and Minerva, Israel; BRF, Norway; Generalitat de Catalunya, Generalitat Valenciana, Spain; the Royal Society and Leverhulme Trust, United Kingdo

Search for dark matter in association with a Higgs boson decaying to b-quarks in pp collisions at √s=13 TeV with the ATLAS detector

A search for dark matter pair production in association with a Higgs boson decaying to a pair of bottom quarks is presented, using 3.2 fb−1of pp collisions at a centre-of-mass energy of 13 TeV collected by the ATLAS detector at the LHC. The decay of the Higgs boson is reconstructed as a high-momentum bb¯ system with either a pair of small-radius jets, or a single large-radius jet with substructure. The observed data are found to be consistent with the expected backgrounds. Results are interpreted using a simplified model with a Z′gauge boson mediating the interaction between dark matter and the Standard Model as well as a two-Higgs-doublet model containing an additional Z′boson which decays to a Standard Model Higgs boson and a new pseudoscalar Higgs boson, the latter decaying into a pair of dark matter particlesWe acknowledge the support of ANPCyT, Argentina; YerPhI, Ar-menia; ARC, Australia; BMWFW and FWF, Austria; ANAS, Azerbai-jan; SSTC, Belarus; CNPq and FAPESP, Brazil; NSERC, NRC and CFI, Canada; CERN; CONICYT, Chile; CAS, MOST and NSFC, China; COLCIENCIAS, Colombia; MSMT CR, MPO CR and VSC CR, Czech Re-public; DNRF and DNSRC, Denmark; IN2P3-CNRS, CEA-DSM/IRFU, France; GNSF, Georgia; BMBF, HGF, and MPG, Germany; GSRT, Greece; RGC, Hong Kong SAR, China; ISF, I-CORE and Benoziyo Center, Israel; INFN, Italy; MEXT and JSPS, Japan; CNRST, Morocco; FOM and NWO, Netherlands; RCN, Norway; MNiSW and NCN, Poland; FCT, Portugal; MNE/IFA, Romania; MES of Russia and NRC KI, Russian Federation; JINR; MESTD, Serbia; MSSR, Slovakia; ARRS and MIZŠ, Slovenia; DST/NRF, South Africa; MINECO, Spain; SRC and Wallenberg Foundation, Sweden; SERI, SNSF and Cantons of Bern and Geneva, Switzerland; MOST, Taiwan; TAEK, Turkey; STFC, United Kingdom; DOE and NSF, United States of America. In addition, individual groups and members have received support from BCKDF, the Canada Council, CANARIE, CRC, Compute Canada, FQRNT, and the Ontario Innovation Trust, Canada; EPLANET, ERC, FP7, Horizon 2020 and Marie Skłodowska-Curie Actions, opean Union; Investissements d’Avenir Labex and Idex, ANR, Région Au-vergne and Fondation Partager le Savoir, France; DFG and AvH Foundation, Germany; Herakleitos, Thales and Aristeia programmes co-financed by EU-ESF and the Greek NSRF; BSF, GIF and Minerva, Israel; BRF, Norway; Generalitat de Catalunya, Generalitat Valen-ciana, Spain; the Royal Society and Leverhulme Trust, United Kingdo

Sliding mode control of switching converters: general theory in an integrated solution

The application of the sliding-mode control theory to the regulation of dc-to-dc switching converters is reviewed. Subsequently, linear dynamical models are derived and compensating networks are designed. An analog integrated circuit illustrating the theory has been designed, implemented and applied to regulate a buck converter in continuous conduction mode. Experimental verifications are provided.Peer ReviewedPostprint (published version

Search for pair production of third-generation scalar leptoquarks decaying into a top quark and a tau-lepton in pp collisions at √s=13 TeV with the ATLAS detector

Artículo escrito por un elevado número de autores, solo se referencian el que aparece en primer lugar, el nombre del grupo de colaboración, si le hubiere, y los autores pertenecientes a la UA