Reducing the nitrate content of protected lettuce.

End of Project ReportA research project was carried out jointly between Teagasc, Kinsealy Research Centre and University College Dublin, Department of Crop Science, Horticulture and Forestry which studied the effects of cultivar, nitrogen fertilisation and light intensity on the nitrate content of protected butterhead lettuce. In a series of cultivar trials of winter and summer butterhead lettuce, significant differences in the nitrate content of the lettuce between cultivars were found only in one experiment. In this instance, the differences were not consistent between successive harvests. It was concluded that screening lettuce cultivars for tissue nitrate level is unlikely to contribute to an overall reduction of nitrate levels. The application of N in a liquid feed throughout the cropping period resulted in higher nitrate levels in lettuce plants grown in soil filled containers compared with a similar amount of N applied to the soil before planting. Withdrawing N for the final 10 days of the cropping period did not affect the nitrate content of the lettuce. In an experiment studying nitrogen source and rate on lettuce grown in containers, the use of calcium cyanamide as a N source resulted in lower nitrate levels in the lettuce and gave a reduced head weight compared with calcium ammonium nitrate (CAN) or ammonium sulphate. Increasing the rate of CAN or ammonium sulphate gave higher lettuce nitrate levels. A nitrification inhibitor reduced the soil nitrate levels especially with sulphate of ammonia as the N source but did not affect the plant nitrate levels significantly. The addition of chloride to the soil reduced nitrate levels in the lettuce. In a further fertilisation study using containers, calcium cyanamide again resulted in lower plant nitrate levels than CAN. Increasing the rate of CAN increased soil nitrate levels, lettuce head weight and plant nitrate levels. The relationship between soil nitrate levels, lettuce head weight and plant nitrate level indicates that the level of 100-150 mg·L-1 of nitrate N in the soil, advocated in the Code of Good Practice, is a compromise between maximising plant growth and minimising lettuce nitrate content. A comparison between CAN and calcium cyanamide in a border soil experiment again showed that the latter N source resulted in lower lettuce nitrate levels. In this experiment the addition of chloride to the soil did not affect plant nitrate levels. Lettuce was grown, in late summer, in small tunnels using a range of polyethylene cladding materials. Head weight correlated well with the overall light transmission of the materials. In one of the materials that had a low light transmission, lettuce nitrate content was doubled compared with those grown under the materials with high light transmission. Under both winter and summer conditions, the nitrate content of lettuce heads was not influenced by the time of day at which harvest took place. In experiments in which multiple harvests were carried out there was no consistent trend in nitrate content as the heads developed and matured. Within individual heads of lettuce there was a steep concentration gradient with the older outer leaves having much higher concentrations of nitrate than the younger inner leaves. Herbicides commonly used in protected lettuce production did not influence the nitrate content of the lettuce

The environmental contaminant DDE fails to influence the outcome of sexual differentiation in the marine turtle Chelonia mydas.

In many turtles, the temperature experienced during the middle of egg incubation determines the sex of the offspring. The implication of steroid sex hormones as the proximate trigger for sex determination opens the possibility that endocrine-disrupting contaminants may also influence the outcome of sexual differentiation. In this study we investigate the potential effects of DDE (a common DDT metabolite) on sexual differentiation of Chelonia mydas (green sea turtle). Four clutches of eggs collected from Heron Island, Queensland, Australia, were treated with DDE at the beginning of the thermosensitive period for sexual determination. An incubation temperature of 28 degrees C or less produces male hatchlings in this species, whereas 30 degrees C or more produces female hatchlings. Dosed eggs were consequently incubated at two temperatures (27.6 degrees C and 30.4 degrees C) on the upper and lower boundaries of the sex determination threshold for this species. DDE, ranging from 3.3 to 66.5 microg, was dissolved in 5, 10, and 25 microl ethanol and applied to eggshells above the embryo. Less than 2.5 ng/g DDE was present in eggs prior to dosing. Approximately 34% of the applied DDE was absorbed in the eggs, but only approximately 8% of applied DDE was found in embryos. Thus, treated eggs, corrected for background DDE, had up to 543 ng/g DDE. The sex ratio at these doses did not differ from what would be expected on consideration of temperature alone. Incubation time, hatching success, incidence of body deformities, hatching size, and weight were also within the limits of healthy developed hatchlings. This indicates that the eggs of C. mydas in the wild with concentrations of DDE less than 543 ng/g should produce hatchlings with relatively high hatching success, survival rate, and normally differentiated gonads

A systematic review of simulation studies which compare existing statistical methods to account for non-compliance in randomised controlled trials

INTRODUCTION: Non-compliance is a common challenge for researchers and may reduce the power of an intention-to-treat analysis. Whilst a per protocol approach attempts to deal with this issue, it can result in biased estimates. Several methods to resolve this issue have been identified in previous reviews, but there is limited evidence supporting their use. This review aimed to identify simulation studies which compare such methods, assess the extent to which certain methods have been investigated and determine their performance under various scenarios.METHODS: A systematic search of several electronic databases including MEDLINE and Scopus was carried out from conception to 30th November 2022. Included papers were published in a peer-reviewed journal, readily available in the English language and focused on comparing relevant methods in a superiority randomised controlled trial under a simulation study. Articles were screened using these criteria and a predetermined extraction form used to identify relevant information. A quality assessment appraised the risk of bias in individual studies. Extracted data was synthesised using tables, figures and a narrative summary. Both screening and data extraction were performed by two independent reviewers with disagreements resolved by consensus.RESULTS: Of 2325 papers identified, 267 full texts were screened and 17 studies finally included. Twelve methods were identified across papers. Instrumental variable methods were commonly considered, but many authors found them to be biased in some settings. Non-compliance was generally assumed to be all-or-nothing and only occurring in the intervention group, although some methods considered it as time-varying. Simulation studies commonly varied the level and type of non-compliance and factors such as effect size and strength of confounding. The quality of papers was generally good, although some lacked detail and justification. Therefore, their conclusions were deemed to be less reliable.CONCLUSIONS: It is common for papers to consider instrumental variable methods but more studies are needed that consider G-methods and compare a wide range of methods in realistic scenarios. It is difficult to make conclusions about the best method to deal with non-compliance due to a limited body of evidence and the difficulty in combining results from independent simulation studies.PROSPERO REGISTRATION NUMBER: CRD42022370910.</p

The Counterpart Principle of Analogical Support by Structural Similarity

We propose and investigate an Analogy Principle in the context of Unary Inductive Logic based on a notion of support by structural similarity which is often employed to motivate scientific conjectures

Mutation analysis of HIF prolyl hydroxylases (PHD/EGLN) in individuals with features of phaeochromocytoma and renal cell carcinoma susceptibility

Germline mutations in the von Hippel–Lindau disease (VHL) and succinate dehydrogenase subunit B (SDHB) genes can cause inherited phaeochromocytoma and/or renal cell carcinoma(RCC). Dysregulation of the hypoxia-inducible factor (HIF) transcription factors has been linked to VHL and SDHB-related RCC; both HIF dysregulation and disordered function of a prolyl hydroxylase domain isoform 3 (PHD3/EGLN3)-related pathway of neuronal apoptosis have been linked to the development of phaeochromocytoma. The 2-oxoglutarate-dependent prolyl hydroxylase enzymes PHD1 (EGLN2), PHD2 (EGLN1) and PHD3 (EGLN3) have a key role in regulating the stability of HIF-a subunits (and hence expression of the HIF-a transcription factors). A germline PHD2 mutation has been reported in association with congenital erythrocytosis and recurrent extra-adrenal phaeochromocytoma. We undertook mutation analysis of PHD1, PHD2 and PHD3 in two cohorts of patients with features of inherited phaeochromocytoma (nZ82) and inherited RCC (nZ64) and no evidence of germline mutations in known susceptibility genes. No confirmed pathogenic mutations were detected suggesting that mutations in these genes are not a frequent cause of inherited phaeochromocytoma or RCC

Tracking and Data Relay Satellite System (TDRSS) Support of User Spacecraft without TDRSS Transponders

NASA GSFC VNS TSG personnel have proposed the use of TDRSS to obtain telemetry and/or S-band one-way return Doppler tracking data for spacecraft which do not have TDRSS-compatible transponders and therefore were never considered candidates for TDRSS support. For spacecraft with less stable local oscillators (LO), one-way return Doppler tracking data is typically of poor quality. It has been demonstrated using UARS, WIND, and NOAA-J tracking data that the simultaneous use of two TDRSS spacecraft can yield differenced one-way return Doppler data of high quality which is usable for orbit determination by differencing away the effects of oscillator instability