Frequency multiplication in high-energy electron beams Semiannual progress report, Oct. 1, 1966 - Apr. 1, 1967

Frequency multiplication in high energy electron beam

The Role of Indigenous Knowledge in the Effective Collective Management of the Communal Rangelands

Numerous scientific studies have highlighted the complexities associated with the collective management of communal rangelands. To date, policy interventions in rangelands have largely ignored people’s traditional ways of managing, with adverse effects on rangeland productivity. Thus, local knowledge has not been considered in spatial planning, despite the fact that local rural communities are often repositories of key indigenous knowledge. Hence this study set out to evaluate the role of indigenous knowledge in the management of the communal rangeland in Cata and Guquka, now and in the future. This was achieved through the use of Participatory GIS (PGIS), specifically participatory mapping to analyse how the communities use and view their rangelands now and how this has changed over time, and whether this can form a potential resource for effective communal rangeland management in the future. Results revealed that Cata and Guquka participants held extensive indigenous and spatial knowledge in relation to their communal areas. However, the existing knowledge is not translated into effective management of the communal rangelands, instead it is trapped in the older generation. These findings were attributed to social challenges including an ageing population, lack of youth involvement, fear of livestock theft, lack of mutual trust amongst community members and lack of resources such as fencing, access to dipping tanks and government services, and financial constraints. Thus, factors inhibiting the use of the existing indigenous knowledge for effective management of the communal rangelands in Cata and Guquka are more social than environmental. This suggests that new policy approaches incorporating local people’s indigenous knowledge in spatial planning which takes into account their unique local situations and the relationships between people and their resources are necessary. When people feel like their voices are heard and opinions valued, the adoption and sustainability of policy-based interventions becomes less challenging. Therefore, indigenous local knowledge, if effectively harnessed, could form a key component in adaptive management of these communal rangelands

Heterologous prime-boost-boost immunisation of Chinese cynomolgus macaques using DNA and recombinant poxvirus vectors expressing HIV-1 virus-like particles

Background: There is renewed interest in the development of poxvirus vector-based HIV vaccines due to the protective effect observed with repeated recombinant canarypox priming with gp120 boosting in the recent Thai placebo-controlled trial. This study sought to investigate whether a heterologous prime-boost-boost vaccine regimen in Chinese cynomolgus macaques with a DNA vaccine and recombinant poxviral vectors expressing HIV virus-like particles bearing envelopes derived from the most prevalent clades circulating in sub-Saharan Africa, focused the antibody response to shared neutralising epitopes. Methods: Three Chinese cynomolgus macaques were immunised via intramuscular injections using a regimen composed of a prime with two DNA vaccines expressing clade A Env/clade B Gag followed by boosting with recombinant fowlpox virus expressing HIV-1 clade D Gag, Env and cholera toxin B subunit followed by the final boost with recombinant modified vaccinia virus Ankara expressing HIV-1 clade C Env, Gag and human complement protein C3d. We measured the macaque serum antibody responses by ELISA, enumerated T cell responses by IFN-gamma ELISpot and assessed seroneutralisation of HIV-1 using the TZM-bl beta-galactosidase assay with primary isolates of HIV-1. Results: This study shows that large and complex synthetic DNA sequences can be successfully cloned in a single step into two poxvirus vectors: MVA and FPV and the recombinant poxviruses could be grown to high titres. The vaccine candidates showed appropriate expression of recombinant proteins with the formation of authentic HIV virus-like particles seen on transmission electron microscopy. In addition the b12 epitope was shown to be held in common by the vaccine candidates using confocal immunofluorescent microscopy. The vaccine candidates were safely administered to Chinese cynomolgus macaques which elicited modest T cell responses at the end of the study but only one out of the three macaques elicited an HIV-specific antibody response. However, the antibodies did not neutralise primary isolates of HIV-1 or the V3-sensitive isolate SF162 using the TZM-bl b-galactosidase assay. Conclusions: MVA and FP9 are ideal replication-deficient viral vectors for HIV-1 vaccines due to their excellent safety profile for use in humans. This study shows this novel prime-boost-boost regimen was poorly immunogenic in Chinese cynomolgus macaques

Out of Step with the World

What are we to make of the cultural nonconformity of hardcore/punks? Is there any ethical value in the pursuit of cultural nonconformity? Distinct moral justifications can be teased from the lyrics of the hardcore/punk bands that we have grown up with and still love. The best explanation of what makes cultural nonconformity morally valuable, we believe, comes from John Stuart Mill: that it opens up new cultural space to oneself and to others, permitting "new and original experiments of living.

Initial Studies of the Bidirectional Reflectance Distribution Function of Multi-Walled Carbon Nanotube Structures for Stray Light Control Applications

The presentation examines the application of low reflectance surfaces in optical instruments, multi-walled carbon nanotubes (MWCNTs), research objects, MWCNT samples, measurement of 8 deg. directional/hemispherical reflectance, measurement of bidirectional reflectance distribution function (BRDF), and what is current the "blackest ever black"

Plasma Electronics

Contains reports on nine research projects.U. S. Air Force under Air Force Contract AF 19(604)-7400National Science Foundation under Grant G-9330U.S.Navy(Office of Naval Research)under Contract Nonr-1841(78)U. S. ArmyLincoln Laboratory, Purchase Order DDL B-00337U. S. Nav

Plasma Electronics

Contains research objectives and reports on twelve research projects.National Science Foundation under Grant G-9330U. S. Navy (Office of Naval Research) under Contract Nonr-1841(78)U. S. NavyLincoln Laboratory, Purchase Order DDL B-00306U. S. ArmyU. S. Air Force under Air Force Contract AF19(604)-740

Plasma Electronics

Contains reports on eight research projects.National Science Foundation (Grant G-24073)United States Atomic Energy Commission (Contract AF(30-1)-3285)Lincoln Laboratory (Purchase Order DDL BB-107)United States Air Force (Contract AF19(628)-500)United States Atomic Energy Commission (Contract AT(30-1)-3221

Plasmas and Controlled Nuclear Fusion

Contains reports on two research projects.National Science Foundation (Grants GK-57)National Science Foundation (Grants GK-614

Laser Time-of-Flight Mass Spectrometry for Future In Situ Planetary Missions

Laser desorption/ionization time-of-flight mass spectrometry (LD-TOF-MS) is a versatile, low-complexity instrument class that holds significant promise for future landed in situ planetary missions that emphasize compositional analysis of surface materials. Here we describe a 5kg-class instrument that is capable of detecting and analyzing a variety of analytes directly from rock or ice samples. Through laboratory studies of a suite of representative samples, we show that detection and analysis of key mineral composition, small organics, and particularly, higher molecular weight organics are well suited to this instrument design. A mass range exceeding 100,000 Da has recently been demonstrated. We describe recent efforts in instrument prototype development and future directions that will enhance our analytical capabilities targeting organic mixtures on primitive and icy bodies. We present results on a series of standards, simulated mixtures, and meteoritic samples