Stormwater sand filters in water-sensitive urban design

This paper investigates the suitability of sand filters for harvesting and treating stormwater for non-potable reuse purposes. A stormwater sand filtration device was constructed in a small urban catchment in Sydney, Australia. A sand filter is typically used in water-sensitive urban design (WSUD) as a component of a treatment train to remove pollution from stormwater before discharge to receiving waters, to groundwater or for collection and reuse. This paper describes an 18 month field study undertaken to determine the effectiveness and pollutant removal efficiency of a sand filter, and the differences in the pollutant removal efficiency of two grades of sand. A comparison of pollutant removal with previous literature on sand filters showed similar efficiencies but nutrient removal was higher than expected. A further unexpected result was that the coarse filter media performed as well as the fine media for most pollutant types and was superior in suspended solids removal. Improved modelling equations for predicting suspended solids and total phosphorus removal in sand filters are also presented in this paper

Spectroscopy and Imaging Performance of the Liquid Xenon Gamma-Ray Imaging Telescope (LXeGRIT)

LXeGRIT is a balloon-borne Compton telescope based on a liquid xenon time projection chamber (LXeTPC) for imaging cosmic \g-rays in the energy band of 0.2-20 MeV. The detector, with 400 cm$^2$ area and 7 cm drift gap, is filled with high purity LXe. Both ionization and scintillation light signals are detected to measure the energy deposits and the three spatial coordinates of individual \g -ray interactions within the sensitive volume. The TPC has been characterized with repeated measurements of its spectral and Compton imaging response to \g -rays from radioactive sources such as \na, \cs, \yt and Am-Be. The detector shows a linear response to \g -rays in the energy range 511 keV -4.4 MeV, with an energy resolution (FWHM) of \Delta E/E=8.8% \: \sqrt{1\MeV /E}. Compton imaging of \yt \g -ray events with two detected interactions is consistent with an angular resolution of $\sim$ 3 degrees (RMS) at 1.8 MeV.Comment: To appear in: Hard X-Ray, Gamma-Ray and Neutron Detector Physics XI, 2000; Proc. SPIE, vol. 4140; K.A. Flanagan & O.H. Siegmund, ed

MiR203 Mediates Subversion of Stem Cell Properties During Mammary Epithelial Differentiation via Repression of ΔNP63α and Promotes Mesenchymal-to-Epithelial Transition

During reproductive life, the mammary epithelium undergoes consecutive cycles of proliferation, differentiation and apoptosis. Doing so relies on the retained proliferative capacity, prolonged lifespan and developmental potency of mammary stem cells (MaSCs). ΔNp63α, the predominant TP63 isoform in mammary epithelia, is robustly expressed in MaSCs and is required for preservation of self-renewing capacity in diverse epithelial structures. However, the mechanism(s) underlying subversion of this activity during forfeiture of self-renewing capacity are poorly understood. MicroRNAs (miRNAs) govern critical cellular functions including stem cell maintenance, development, cell cycle regulation and differentiation by disrupting translation of target mRNAs. Data presented here indicate that expression of miR203, a miRNA that targets ΔNp63α and ΔNp63β is activated during luminal epithelial differentiation and that this pattern is observed in the murine mammary hierarchy. In addition, we present evidence that the transcription factor Zeb1 represses miR203 expression, thus enhancing ΔNp63α protein levels. Furthermore, ectopic miR203 suppresses ΔNp63α expression, proliferation and colony formation. The anti-clonogenic effects mediated by miR203 require suppression of ΔNp63α. In addition, ectopic miR203 promotes mesenchymal-to-epithelial transition and disrupts activities associated with epithelial stem cells. These studies support a model in which induction of miR203 mediates forfeiture of self-renewing capacity via suppression of ΔNp63α and may also have anti-tumorigenic activity through its reduction of EMT and cancer stem cell populations

Gamma-ray observations of the Crab Region using a coded-aperture telescope

The region of the Galactic anticenter, including the Crab Nebula, was observed during a balloon flight of the University of New Hampshire Directional Gamma-Ray Telescope employing the coded-aperture imaging technique to image celestial gamma-radiation between 160 keV and 9.3 MeV. The background systematics are treated with a simple and relatively straightforward correction procedure. The results demonstrate that the coded-aperture procedure is a viable approach for imaging not only point sources of radiation, but also extended sources of emission. The results for the Crab\u27s photon spectrum are consistent with a power-law spectrum. Upper limits on the flux levels of line emission at 405 keV and 1050 keV and on the flux from the X-ray binary source A0535 + 26 and diffuse Galactic emission from the anticenter region are derived

Oncogenic Transformation of Mammary Epithelial Cells by Transforming Growth Factor Beta Independent of Mammary Stem Cell Regulation

BackgroundTransforming growth factor beta (TGFβ) is transiently increased in the mammary gland during involution and by radiation. While TGFβ normally has a tumour suppressor role, prolonged exposure to TGFβ can induce an oncogenic epithelial to mesenchymal transition (EMT) program in permissive cells and initiate the generation of cancer stem cells. Our objective is to mimic the transient exposure to TGFβ during involution to determine the persistent effects on premalignant mammary epithelium

Identification of Protein Biomarker Signatures for Acute Myeloid Leukemia (AML) Using Both Nontargeted and Targeted Approaches

Acute myeloid leukemia (AML) is characterized by an increasing number of clonal myeloid blast cells which are incapable of differentiating into mature leukocytes. AML risk stratification is based on genetic background, which also serves as a means to identify the optimal treatment of individual patients. However, constant refinements are needed, and the inclusion of significant measurements, based on the various omics approaches that are currently available to researchers/clinicians, have the potential to increase overall accuracy with respect to patient management. Using both nontargeted (label-free mass spectrometry) and targeted (multiplex immunoassays) proteomics, a range of proteins were found to be significantly changed in AML patients with different genetic backgrounds. The inclusion of validated proteomic biomarker panels could be an important factor in the prognostic classification of AML patients. The ability to measure both cellular and secreted analytes, at diagnosis and during the course of treatment, has advantages in identifying transforming biological mechanisms in patients, assisting important clinical management decisions.Peer reviewe

Gamma-ray observations of Cygnus X-1 and Cygnus X-3 using a coded-aperture telescope

A balloon-borne coded-aperture telescope, measuring gamma-ray photons in the 160 keV to 9.3 MeV range, was used to observe the Cygnus region of the sky on October 1 and 2, 1984. In the 2-9.3-MeV band, evidence is found for a hard spectral component with a mean flux level at the top of the atmosphere of 7.4 + or - 2.5 x 10 to the -7th photons/sq cm per s per keV, inconsistent with the predictions of the inverse Compton models normally used to describe the X-ray emission. Both Cyg X-1 and Cyg X-3 could be observed simultaneously with the telescope. The results are used to establish 1-sigma upper flux limits on the spectral emission from Cyg X-3

Pregnancy Induces Persistent Changes that Potentiate Apoptotic Signaling and Responses to DNA Damage

A full-term pregnancy reduces the lifetime risk of breast cancer by up to 50%. This effect is mediated, in part, by p53-dependent pathways. Gene expression profiling was used to investigate the mechanisms that alter apoptotic responses to DNA damage in the mammary gland. Radiation-induced responses in BALB/c-Trp53+/+ and BALB/c-Trp53-/- mice identified 121 genes that were altered by radiation and p53 status (p53-IR). To determine the effect of parity, mice were mated, force-weaned and mammary glands were allowed to involute for 21 days (parous) and compared with age-matched nulliparous mice. Gene expression profiles were determined in mammary tissues from nulliparous (N), parous (P), irradiated nulliparous (N-IR) and irradiated parous (P-IR) mice. The p53-IR gene signature did not differ among the N-IR and P-IR groups indicating that transcriptional activity of p53 was not altered by parity. However, expression profiles of apoptosis-related genes differed significantly in the parous group. The alterations in parous mammary tissues was accompanied by over-representation of biological processes that included “signal transduction” (e=1.69E-05). Within this set, Wnt signaling was especially pronounced (e Parity-regulated genes collaborate with p53-dependent targets, which act as a “switch”, to elicit apoptosis following ionizing radiation. The epigenetic states of the parity-regulated genes Tgfb2 and Wnt5a provide a mechanism for the persistent alterations in gene expression and apoptosis in parous mammary epithelial cells

Mapping Children's Discussions of Evidence in Science to Assess Collaboration and Argumentation

The research reported in this paper concerns the development of children's skills of interpreting and evaluating evidence in science. Previous studies have shown that school teaching often places limited emphasis on the development of these skills, which are necessary for children to engage in scientific debate and decision-making. The research, undertaken in the UK, involved four collaborative decision-making activities to stimulate group discussion, each was carried out with five groups of four children (10-11 years old). The research shows how the children evaluated evidence for possible choices and judged whether their evidence was sufficient to support a particular conclusion or the rejection of alternative conclusions. A mapping technique was developed to analyse the discussions and identify different "levels" of argumentation. The authors conclude that suitable collaborative activities that focus on the discussion of evidence can be developed to exercise children's ability to argue effectively in making decisions