Performance of CO1 and ITS2 nested PCR in molecular identification of ordinary scabies (Sarcoptes scabiei var. hominis)

Scabies is a global disease with a high prevalence, causing morbidity and even mortality, especially in poor and developing countries. However, it is often misdiagnosed due to varied and unspecified lesions. The gold standard technique for diagnosis is a microscopic examination, which requires experienced experts in finding mites, mainly in ordinary scabies. CO1 and ITS2 genes have been widely used in molecular identification to detect Sarcoptes scabiei and its variants. This study aimed to determine and compare the sensitivity and specificity of CO1 and ITS2 S. scabiei genes to the microscopic examination of scabies skin scrapings. The skin scrapings of 52 subjects with scabies diagnosed by anamnesis, physical examination, and dermoscopic examination were examined under a microscope and analyzed by nested PCR. The diagnostic test result showed that the sensitivity of nested PCR of both CO1 and ITS2 genes to micro‐ scope examination was 100%. However, the specificity of both CO1 and ITS2 nested PCR was poor (24% and 0%). Hence, CO1 and ITS2 nested PCR could be more suitable for screening ordinary scabies in humans than the microscopic examination

Acquired haemophilia A treated with recombinant factor VIIa by an infusion pump and midline catheter

Acquired haemophilia A is an autoimmune bleeding disorder treated with immunosuppression and bypassing agents such as recombinant factor VIIa (rFVIIa). The half time of rFVIIa is short, which requires frequent bolus injections in order to maintain hemostasis. Providing a continuous pump infusion instead of bolus injections could not only be more time and cost- -efficient but also safer by maintaining a constant level of the bypassing agent. rFVIIa is administered intravenously usually through a peripheral venous catheter. In patients with prolonged intravenous treatment or difficult-to-access peripheral vasculature, midline long peripheral intravenous catheters are an interesting alternative. They have favourable dwell times and failure rates while maintaining the same risk of infection as other peripherally inserted central catheters. This technique has the potential to reduce the costs as well as risk to the patients. Herein, we report a case of a patient with AHA, who was treated with rFVIIa by an infusion pump and midline catheter.Acquired haemophilia A is an autoimmune bleeding disorder treated with immunosuppression and bypassing agents such as recombinant factor VIIa (rFVIIa). The half time of rFVIIa is short, which requires frequent bolus injections in order to maintain hemostasis. Providing a continuous pump infusion instead of bolus injections could not only be more time and cost- -efficient but also safer by maintaining a constant level of the bypassing agent. rFVIIa is administered intravenously usually through a peripheral venous catheter. In patients with prolonged intravenous treatment or difficult-to-access peripheral vasculature, midline long peripheral intravenous catheters are an interesting alternative. They have favourable dwell times and failure rates while maintaining the same risk of infection as other peripherally inserted central catheters. This technique has the potential to reduce the costs as well as risk to the patients. Herein, we report a case of a patient with AHA, who was treated with rFVIIa by an infusion pump and midline catheter

Intrasellar abscess after transsphenoidal pituitary surgery

Sellar abscess is extremely rare complication that could occur after a transphenoidal surgery (TSS). Here we report 45-year-old female patient with left cheek numbness for one month. The Magnetic resonance imaging (MRI) report suggested a pituitary adenoma. Therefore, transphenoidal surgery was performed. During follow up, the patient complained of headache for few days. Subsequent MRI revealed recurrent or residual pituitary tumor. We attempted to excise the lesion using a transfrontal approach. However, pus in the sellar region was found at operation, and no tumor tissue was identified. After the surgery, the patient received antibiotic therapy and fully recovered. Sellar abscess is a serious condition with a high mortality rate. However, a combination of surgical resection and specific antibiotic therapy seems to be very promising

Acquired haemophilia A treated with recombinant factor VIIa by an infusion pump and midline catheter - case study

Nabyta hemofilia A to skaza krwotoczna o podłożu autoimmunologicznym, której leczenieobejmuje immunosupresję i leczenie omijające inhibitor, między innymi rekombinowanymczynnikiem VIIa (rFVIIa). Czas półtrwania rFVIIa jest krótki, co wymaga częstej podażyleku w celu utrzymania hemostazy. Zapewnienie ciągłego wlewu z pompy infuzyjnej zamiastpodaży bolusów może się okazać nie tylko korzystne czasowo i finansowo, ale również bardziejbezpieczne dla pacjenta ze względu na możliwość utrzymania stałego poziomu czynnika omijającegoinhibitor.Zazwyczaj rFVIIa jest podawany dożylnie poprzez cewnik wprowadzany obwodowo. Ciekawąalternatywę dla pacjentów poddawanym leczeniu dożylnemu lub dla osób z trudnym dostępemnaczyniowym stanowi wkłucie pośrednie (midline insertion). Założenie takiego wkłucia jestbezpieczniejsze dla pacjenta, a ryzyko infekcji można porównać z ryzykiem przy zastosowaniuinnych obwodowych cewników żylnych. Zastosowanie techniki wkłucia pośredniego stanowimniejsze zagrożenie dla pacjentów i pozwala obniżyć koszty leczenia.W niniejszym artykule przedstawiono przypadek pacjentki z nabytą hemofilią A, której podawanorFVIIa przez cewnik pośredni (midline) przy użyciu pompy infuzyjnej.Acquired haemophilia A is an autoimmune bleeding disorder treated with immunosuppression and bypassing agents such as recombinant factor VIIa (rFVIIa). The half time of rFVIIa is short, which requires frequent bolus injections in order to maintain hemostasis. Providing a continuous pump infusion instead of bolus injections could not only be more time and cost-efficient but also safer by maintaining a constant level of the bypassing agent. rFVIIa is administered intravenously usually through a peripheral venous catheter. In patients with prolonged intravenous treatment or difficult-to-access peripheral vasculature, midline long peripheral intravenous catheters are an interesting alternative. They have favourable dwell times and failure rates while maintaining the same risk of infection as other peripherally inserted central catheters. This technique has potential to reduce the costs as well as risk to the patients. Herein, we report patient with AHA, who was treated with rFVIIa by an infusion pump and midline catheter

Radial and time resolved measurement of electron density in a copper vapour laser

International audienc

SATURNE LATEST RESULTS FOR POLARIZED BEAMS

Les intensités en protons et en deutons ont pu être progressivement portées à 2.1011 particules/cycle depuis la mise en service de MIMAS en 1987 et grâce aux améliorations faites sur la source. Il n'y a pas de résonance de dépolarisation en deutons. Les résonances de dépolarisation en protons dans MIMAS et SATURNE ont fait l'objet d'études systématiques jusqu'à l'énergie maximale de SATURNE (2.91 GeV). Compte tenu des améliorations encore possibles sur la source et moyennant une injection a énergie plus élevée dans MIMAS une intensité de 5.1011 p ou d par cycle est possible.The intensities for polarized protons or deuterons have been reached up to 2.1011 p or d by cycle since MIMAS operation in 1987 and also with the last improvements of the source. There is no depolarization for deuterons but for protons all the depolarizations in MIMAS and SATURNE have been studied up to the maximum energy. With the possible improvements on the source, associated to an higher injection energy into MIMAS, intensities up to 5.1011 p or d are possible