DIVERSITY OF THE SOUTHERN GREEN STINK BUG NEZARA VIRIDULA (L.) (HETEROPTERA: PENTATOMIDAE)

The southern green stink bug Nezara viridula (L.) (Heteroptera: Pentatomidae) is a global pest of considerable ecological, agricultural and economical interest. The ancestral home of this species is supposed to be Africa and/or Mediterranean and presumably it was spread worldwide during the last two centuries with human trade and agriculture. Bugs found today on different continents do not differ morphologically, however there are substantial differences in their mating behaviour. We used horizontal starch gel electrophoresis to determine the suitability of biochemical markers for assessment of genetic variation between geographically isolated populations of N. viridula. The initial survey of populations from Slovenia, France, French West Indies and Brazil resulted in the resolution of polymorphic banding patterns within the following enzyme systems: GPI, IDH, MDH, ME, MPI and PGM. Results indicate there are consistent differences among tested populations

DIVERSITY OF THE SOUTHERN GREEN STINK BUG NEZARA VIRIDULA (L.) (HETEROPTERA: PENTATOMIDAE)

The southern green stink bug Nezara viridula (L.) (Heteroptera: Pentatomidae) is a global pest of considerable ecological, agricultural and economical interest. The ancestral home of this species is supposed to be Africa and/or Mediterranean and presumably it was spread worldwide during the last two centuries with human trade and agriculture. Bugs found today on different continents do not differ morphologically, however there are substantial differences in their mating behaviour. We used horizontal starch gel electrophoresis to determine the suitability of biochemical markers for assessment of genetic variation between geographically isolated populations of N. viridula. The initial survey of populations from Slovenia, France, French West Indies and Brazil resulted in the resolution of polymorphic banding patterns within the following enzyme systems: GPI, IDH, MDH, ME, MPI and PGM. Results indicate there are consistent differences among tested populations

GMO sampling strategies in food and feed chains

The sampling step is relevant whenever it is necessary to evaluate an analyte in a lot. However, sampling is by far the most crucial step when the analyte is heterogeneously distributed in the lot, especially at low concentration, because high sampling uncertainty could invalidate the overall analytical testing. The economic and legal implications of such failure could greatly exceed the very high cost of accurate sampling. When there is a legal requirement that the analyte be analytically traced, low sampling reliability at any step of the production and distribution chains could cause the entire traceability system to fail. These considerations also apply to the legally - imposed GMO requirements, particularly those for labelling and traceability. Improper sampling can mislead both food producers and consumers about the GM content in products. In addition, if GMO tests fail to detect an unauthorised GM crop, those unauthorised crops could pose potential risks to human and animal health. Failure of traceability could result in collapse of transgenic and conventional/organic co - existence in the fi eld, with subsequent environmental and economic consequences. A sampling plan should be reliable, cost - effective and feasible in the real life. ‘ Fit - for - purpose ’ sampling plans should be studied and applied, given the wide variety of field situations and ultimate goals. In addition, the uncertainty level of the ‘ overall analytical chain ’ is relevant in many respects, such as making decisions about the best procedures to put in place. Uncertainty in GMO sampling is high, therefore statistical studies should be performed to evaluate sampling uncertainty or at least generate insight into how to make responsible decisions on the most appropriate sampling plan to adopt at each step of the food and feed chain. Prior to the European research project Co - Extra, valuable and indispensible research had been conducted on topics such as the distribution of GMOs in a lot and the implementation of reliable software tools to reveal the most suitable sample size for GMO detection. Research conducted within the Co - Extra project fi lled in relevant theoretical gaps related to the assessment of control plans. All of this research represents a basis for selecting appropriate sampling plans that are best adapted to the operational conditions.JRC.I.3-Molecular Biology and Genomic