15 research outputs found

    ANO1 amplification and expression in HNSCC with a high propensity for future distant metastasis and its functions in HNSCC cell lines

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    BACKGROUND: Head and neck squamous cell carcinoma (HNSCC) is associated with poor survival. To identify prognostic and diagnostic markers and therapeutic targets, we studied ANO1, a recently identified calcium-activated chloride channel (CaCC). METHODS: High-resolution genomic and transcriptomic microarray analysis and functional studies using HNSCC cell line and CaCC inhibitors. RESULTS: Amplification and overexpression of genes within the 11q13 amplicon are associated with the propensity for future distance metastasis of HPV-negative HNSCC. ANO1 was selected for functional studies based on high correlations, cell surface expression and CaCC activity. ANO1 overexpression in cells that express low endogenous levels stimulates cell movement, whereas downregulation in cells with high endogenous levels has the opposite effect. ANO1 overexpression also stimulates attachment, spreading, detachment and invasion, which could account for its effects on migration. CaCC inhibitors decrease movement, suggesting that channel activity is required for the effects of ANO1. In contrast, ANO1 overexpression does not affect cell proliferation. INTERPRETATION: ANO1 amplification and expression could be markers for distant metastasis in HNSCC. ANO1 overexpression affects cell properties linked to metastasis. Inhibitors of CaCCs could be used to inhibit the tumourigenic properties of ANO1, whereas activators developed to increase CaCC activity could have adverse effects

    État des travaux normatifs dans le domaine du mesurage des radionucléides dans l'environnement

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    Les préoccupations concernant la présence de substances radioactives dans l'environnement conduisent régulièrement à des expertises sur les niveaux d'activité des radionucléides dans l'environnement et à des évaluations de leurs impacts radiologiques sur les populations humaines. La crédibilité de ces expertises repose en partie sur la qualité et la fiabilité des mesurages. Ce document présente les systèmes d'organisations de la normalisation, nationale et internationale, ainsi que les documents normatifs publiés et les travaux de normalisation en cours relatifs à la métrologie des radionucléides présents dans les matrices environnementales
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