Quantitative analysis of acetyl-CoA production in hypoxic cancer cells reveals substantial contribution from acetate

Background Cell growth requires fatty acids for membrane synthesis. Fatty acids are assembled from 2-carbon units in the form of acetyl-CoA (AcCoA). In nutrient and oxygen replete conditions, acetyl-CoA is predominantly derived from glucose. In hypoxia, however, flux from glucose to acetyl-CoA decreases, and the fractional contribution of glutamine to acetyl-CoA increases. The significance of other acetyl-CoA sources, however, has not been rigorously evaluated. Here we investigate quantitatively, using 13C-tracers and mass spectrometry, the sources of acetyl-CoA in hypoxia.<p></p> Results In normoxic conditions, cultured cells produced more than 90% of acetyl-CoA from glucose and glutamine-derived carbon. In hypoxic cells, this contribution dropped, ranging across cell lines from 50% to 80%. Thus, under hypoxia, one or more additional substrates significantly contribute to acetyl-CoA production. 13C-tracer experiments revealed that neither amino acids nor fatty acids are the primary source of this acetyl-CoA. Instead, the main additional source is acetate. A large contribution from acetate occurs despite it being present in the medium at a low concentration (50–500 μM).<p></p> Conclusions Acetate is an important source of acetyl-CoA in hypoxia. Inhibition of acetate metabolism may impair tumor growth.<p></p&gt

Nonlinear force-free field modeling of a solar active region using SDO/HMI and SOLIS/VSM data

We use SDO/HMI and SOLIS/VSM photospheric magnetic field measurements to model the force-free coronal field above a solar active region, assuming magnetic forces to dominate. We take measurement uncertainties caused by, e.g., noise and the particular inversion technique into account. After searching for the optimum modeling parameters for the particular data sets, we compare the resulting nonlinear force-free model fields. We show the degree of agreement of the coronal field reconstructions from the different data sources by comparing the relative free energy content, the vertical distribution of the magnetic pressure and the vertically integrated current density. Though the longitudinal and transverse magnetic flux measured by the VSM and HMI is clearly different, we find considerable similarities in the modeled fields. This indicates the robustness of the algorithm we use to calculate the nonlinear force-free fields against differences and deficiencies of the photospheric vector maps used as an input. We also depict how much the absolute values of the total force-free, virial and the free magnetic energy differ and how the orientation of the longitudinal and transverse components of the HMI- and VSM-based model volumes compares to each other.Comment: 9 pages, 5 figure

The Role of Phase Space in Complex Fragment Emission from Low to Intermediate Energies

The experimental emission probabilities of complex fragments by low energy compound nuclei and their dependence upon energy and atomic number are compared to the transition state rates. Intermediate-mass-fragment multiplicity distributions for a variety of reactions at intermediate energies are shown to be binomial and thus reducible at all measured transverse energies. From these distributions a single binary event probability can be extracted which has a thermal dependence. A strong thermal signature is also found in the charge distributions. The n-fold charge distributions are reducible to the 1-fold charge distributions through a simple scaling dictated by fold number and charge conservation.Comment: 15 pages, TeX type, psfig, also available at http://csa5.lbl.gov/moretto/ps/brazil.ps, to appear in Proceedings of the 1st International Conference on Nuclear Dynamics at Long and Short Distances, April 8-12, 1996, Angra dos Reis, Brazi

Quantum speed limit for relativistic spin-0 and spin-1 bosons on commutative and noncommutative planes

Quantum speed limits of relativistic charged spin-0 and spin-1 bosons in the background of a homogeneous magnetic field are studied on both commutative and oncommutative planes. We show that, on the commutative plane, the average speeds of wave packets along the radial direction during the interval in which a quantum state evolving from an initial state to the orthogonal final one can not exceed the speed of light, regardless of the intensities of the magnetic field. However, due to the noncommutativity, the average speeds of the wave packets on noncommutative plane will exceed the speed of light in vacuum provided the intensity of the magnetic field is strong enough. It is a clear signature of violating Lorentz invariance in quantum mechanics region.Comment: 8 pages, no figures. arXiv admin note: text overlap with arXiv:1702.0316

Eigenstates of Paraparticle Creation Operators

Eigenstates of the parabose and parafermi creation operators are constructed. In the Dirac contour representation, the parabose eigenstates correspond to the dual vectors of the parabose coherent states. In order $p=2$, conserved-charge parabose creation operator eigenstates are also constructed. The contour forms of the associated resolutions of unity are obtained.Comment: 14 pages, LaTex file, no macros, no figure

The Pairwise Peculiar Velocity Dispersion of Galaxies: Effects of the Infall

We study the reliability of the reconstruction method which uses a modelling of the redshift distortions of the two-point correlation function to estimate the pairwise peculiar velocity dispersion of galaxies. In particular, the dependence of this quantity on different models for the infall velocity is examined for the Las Campanas Redshift Survey. We make extensive use of numerical simulations and of mock catalogs derived from them to discuss the effect of a self-similar infall model, of zero infall, and of the real infall taken from the simulation. The implications for two recent discrepant determinations of the pairwise velocity dispersion for this survey are discussed.Comment: minor changes in the discussion; accepted for publication in ApJ; 8 pages with 2 figures include

Dirac quasinormal frequencies in Schwarzschild-AdS space-time

We investigate the quasinormal mode frequencies for the massless Dirac field in static four dimensional $AdS$ space-time. The separation of the Dirac equation is achieved for the first time in $AdS$ space. Besides the relevance that this calculation can have in the framework of the $AdS/CFT$ correspondence between M-theory on $AdS_4\times S^7$ and SU(N) super Yang-Mills theory on $M_3$, it also serves to fill in a gap in the literature, which has only been concerned with particles of integral spin $0,1,2$.Comment: 13 pages, 6 figure

Tilt-Induced Anisotropic to Isotropic Phase Transition at ν=5/2\nu = 5/2

A modest in-plane magnetic field \Bpar\ is sufficient to destroy the fractional quantized Hall states at $\nu = 5/2$ and 7/2 and replace them with anisotropic compressible phases. Remarkably, we find that at larger \Bpar\ these anisotropic phases can themselves be replaced by isotropic compressible phases reminiscent of the composite fermion fluid at $\nu = 1/2$. We present strong evidence that this transition is a consequence of the mixing of Landau levels from different electric subbands. We also report surprising dependences of the energy gaps at $\nu = 5/2$ and 7/3 on the width of the confinement potential.Comment: Accepted by Phys. Rev. Lett. This is a final version with rewritten introduction and modified figure

Self-Diffusion in 2D Dusty Plasma Liquids: Numerical Simulation Results

We perform Brownian dynamics simulations for studying the self-diffusion in two-dimensional (2D) dusty plasma liquids, in terms of both mean-square displacement and velocity autocorrelation function (VAF). Super-diffusion of charged dust particles has been observed to be most significant at infinitely small damping rate $\gamma$ for intermediate coupling strength, where the long-time asymptotic behavior of VAF is found to be the product of $t^{-1}$ and $\exp{(-\gamma t)}$. The former represents the prediction of early theories in 2D simple liquids and the latter the VAF of a free Brownian particle. This leads to a smooth transition from super-diffusion to normal diffusion, and then to sub-diffusion with an increase of the damping rate. These results well explain the seemingly contradictory scattered in recent classical molecular dynamics simulations and experiments of dusty plasmas.Comment: 10 pages 5 figures, accepted by PR