\u3ci\u3eIn Vitro\u3c/i\u3e Gas Production Technique to Predict DMD of Ensiled Forage Ruminant Based Diets

The in vitro gas production technique was used as a tool to develop an improved prediction model of dry matter digestibility of ensiled forage based diets. Eleven diets were tested through conventional experiments of in vivo dry matter digestibility (DMD). The same diets were evaluated by the in vitro gas production technique using a gas pressure transducer . The parameters of the model y = A - B Qt Z√t were calculated with data from the accumulated gas curves, i.e. y=cumulative gas production (ml), Q=e-b, Z=e-c, B=ebT+c√T , being A the value for gas pool size (ml), plus lag time, minimum lag time, time for 50% gas production and time for 95% gas production. A stepwise linear regression procedure was used to obtain a model with the best fit. The higher adjusted R2 (0.85) was obtained for a model with: 1. accumulated gas, 2. lag time, 3. Q, 4. B, 5. time for 50% gas production and 6. minimum lag time were included in the model

Mechanosensitivity of the BK Channels in Human Glioblastoma Cells: Kinetics and Dynamical Complexity

BK channels are potassium selective and exhibit large single-channel conductance. They play an important physiological role in glioma cells: they are involved in cell growth and extensive migrating behavior. Due to the fact that these processes are accompanied by changes in membrane stress, here, we examine mechanosensitive properties of BK channels from human glioblastoma cells (gBK channels). Experiments were performed by the use of patch-clamp method on excised patches under membrane suction (0–40 mmHg) at membrane hyper- and depolarization. We have also checked whether channel’s activity is affected by possible changes of membrane morphology after a series of long impulses of suction. Unconventionally, we also analyzed internal structure of the experimental signal to make inferences about conformational dynamics of the channel in stressed membranes. We examined the fractal long-range memory effect (by R/S Hurst analysis), the rate of changes in information by sample entropy, or correlation dimension, and characterize its complexity over a range of scales by the use of Multiscale Entropy method. The obtained results indicate that gBK channels are mechanosensitive at membrane depolarization and hyperpolarization. Prolonged suction of membrane also influences open–closed fluctuations—it decreases channel’s activity at membrane hyperpolarization and, in contrary, increases channel’s activity at high voltages. Both membrane strain and its “fatigue” reduce dynamical complexity of channel gating, which suggest decrease in the number of available open conformations of channel protein in stressed membranes

Performance of Dairy Goats to Alfalfa Silage Based Diets Supplemented with Different Sources of Carbohydrates

Lactating Saanen dairy goats fed alfalfa silage (AS) based diets in four 4x4 Latin Square designed experiment were studied for the effects of supplementation of three different type of carbohydrates (wheat grain, (W); sorghum grain, (SG) and dry citrus pulp, (DCP)) on milk yield, composition and chewing activities. Sixteen does (45± 10 DIM and 2.016 kg ± 0.48 4% FCM) housed indoors in individual pens in a four 4x4 experiment were fed four diets 1) AS (33.9%DM, 19.9%CP, 44.01%NDF); 2) ASW (52.4%DM, 19.8%CP, 33.7%NDF); 3) ASSG (50.9%DM, 17.9%CP, 37%NDF), 4) ASDCP (52.5%DM, 16.12%CP, 39.1%NDF) with forage-to-concentrate ratios of 100:0 or 65:35, 67:33 and 64:36 respectively. Intake of AS DM (2.78%BW) was different (P\u3c 0.05) from the other treatments (average 3.53 ±0.07 %BW). Chewing efficiency (min/g NDF per kg BW 0.75) decrease (P\u3c 0.05) as a result of AS substitution or concentrate supplementation without effect (P\u3e 0.05) of carbohydrate type or dietary level of NDF. Milk, 4%FCM and fat-protein-corrected milk yield was affected (P\u3c 0.05) by concentrate supplementation. Either milk protein content (g/l) or yield (g/day) were not affected by treatments. Body weight changes appeared related to concentrate supplementation. Supplementation increase total DM intake, decrease forage DM intake and chewing efficiency and increase producing performance without changing milk composition

On the simple random-walk models of ion-channel gate dynamics reflecting long-term memory

Several approaches to ion-channel gating modelling have been proposed. Although many models describe the dwell-time distributions correctly, they are incapable of predicting and explaining the long-term correlations between the lengths of adjacent openings and closings of a channel. In this paper we propose two simple random-walk models of the gating dynamics of voltage and Ca2+-activated potassium channels which qualitatively reproduce the dwell-time distributions, and describe the experimentally observed long-term memory quite well. Biological interpretation of both models is presented. In particular, the origin of the correlations is associated with fluctuations of channel mass density. The long-term memory effect, as measured by Hurst R/S analysis of experimental single-channel patch-clamp recordings, is close to the behaviour predicted by our models. The flexibility of the models enables their use as templates for other types of ion channel