Spectral analysis of boundary layers in Rayleigh-Benard convection

A combined experimental and numerical study of the boundary layer in a 4:1 aspect-ratio Rayleigh-B\'{e}nard cell over a four-decade range of Rayleigh numbers has been undertaken aimed at gaining a better insight into the character of the boundary layers. The experiments involved the simultaneous Laser Doppler Anemometry (LDA) measurements of fluid velocity at two locations, i.e. in the boundary layer and far away from it in the bulk, for Rayleigh numbers varying between $1.6 \times 10^7$ and $2.4 \times 10^9$. In parallel, direct numerical simulations (DNS) have been performed for the same configuration for Rayleigh numbers between $7.0 \times 10^4$ and $7.7 \times 10^7$. The temperature and velocity probability density functions and the power spectra of the horizontal velocity fluctuations measured in the boundary layer and in the bulk flow are found to be practically identical. Except for the smallest Rayleigh numbers, the spectra in the boundary layer and in the bulk central region are continuous and have a wide range of active scales. This indicates that both the bulk and the boundary layers are turbulent in the $\textrm{Ra}$ number range considered. However, molecular effects can still be observed and the boundary layer does not behave like a classical shear-driven turbulent boundary layer.Comment: 10 pages, 6 figures, Accepted for publication in Phys. Rev.

Changes in Eicosanoid and Tumour Necrosis Factor-α Production by Rat Peritoneal Macrophages During Carrageenin-Induced Peritonitis

Changes and correlations in cytokine and eicosanoid production by blood monocytes, non-purified and purified peritoneal cells during a carrageenin-induced peritonitis were investigated for a period of ten days. The cells were isolated and stimulated in vitro. Cytokine and eicosanoid production of the non-purified fraction increased steadily during peritonitis. During the whole episode of peritonitis the production capacity of granulocytes was very low and hardly any effect on the production capacity of macrophages (Mϕ) was observed. Cytokine and eicosanoid production of the non-purified fraction was mainly due to the presence of Mϕ. The production capacity of the peripheral blood monocytes was not similar to that of the peritoneal Mϕ

Platelet-primed interactions of coagulation and anticoagulation pathways in flow-dependent thrombus formation

Abstract: In haemostasis and thrombosis, platelet, coagulation and anticoagulation pathways act together to produce fibrin-containing thrombi. We developed a microspot-based technique, in which we assessed platelet adhesion, platelet activation, thrombus structure and fibrin clot formation in real time using flowing whole blood. Microspots were made from distinct platelet-adhesive surfaces in the absence or presence of tissue factor, thrombomodulin or activated protein C. Kinetics of platelet activation, thrombus structure and fibrin formation were assessed by fluorescence microscopy. This work revealed: (1) a priming role of platelet adhesion in thrombus contraction and subsequent fibrin formation; (2) a surface-independent role of tissue factor, independent of the shear rate; (3) a mechanism of tissue factor-enhanced activation of the intrinsic coagulation pathway; (4) a local, suppressive role of the anticoagulant thrombomodulin/protein C pathway under flow. Multiparameter analysis using blood samples from patients with (anti)coagulation disorders indicated characteristic defects in thrombus formation, in cases of factor V, XI or XII deficiency; and in contrast, thrombogenic effects in patients with factor V-Leiden. Taken together, this integrative phenotyping approach of platelet–fibrin thrombus formation has revealed interaction mechanisms of platelet-primed key haemostatic pathways with alterations in patients with (anti)coagulation defects. It can help as an important functional add-on whole-blood phenotyping

Fine mapping the CETP region reveals a common intronic insertion associated to HDL-C

Background: Individuals with exceptional longevity and their offspring have significantly larger high-density lipoprotein concentrations (HDL-C) particle sizes due to the increased homozygosity for the I405V variant in the cholesteryl ester transfer protein (CETP) gene. In this study, we investigate the association of CETP and HDL-C further to identify novel, independent CETP variants associated with HDL-C in humans. Methods: We performed a meta-analysis of HDL-C within the CETP region using 59,432 individuals imputed with 1000 Genomes data. We performed replication in an independent sample of 47,866 individuals and validation was done by Sanger sequencing. Results: The meta-analysis of HDL-C within the CETP region identified five independent variants, including an exonic variant and a common intronic insertion. We replicated these 5 variants significantly in an independent sample of 47,866 individuals. Sanger sequencing of the insertion within a single family confirmed segregation of this variant. The strongest reported association between HDL-C and CETP variants, was rs3764261; however, after conditioning on the five novel variants we identified the support for rs3764261 was highly reduced (βunadjusted=3.179 mg/dl (P value=5.25×10−509), βadjusted=0.859 mg/dl (P value=9.51×10−25)), and this finding suggests that these five novel variants may partly explain the association of CETP with HDL-C. Indeed, three of the five novel variants (rs34065661, rs5817082, rs7499892) are independent of rs3764261. Conclusions: The causal variants in CETP that account for the association with HDL-C remain unknown. We used studies imputed to the 1000 Genomes reference panel for fine mapping of the CETP region. We identified and validated five variants within this region that may partly account for the association of the known variant (rs3764261), as well as other sources of genetic contribution to HDL-C

Long-term unsteadiness and large-scale structures in Rayleigh-Bénard convection with and without electromagnetic forcing

This dissertation focuses on turbulent thermal convection, which occurs in a wide range of (geo)physical situations, like in the atmosphere, the oceans, the interior of stars or planets, and engineering applications, like metal casting or crystal growth processes. In this work, a special type of thermal convection, Rayleigh-Bénard convection, is studied with and without an additional electromagnetic body force. Experiments are performed in a rectangular RB convection cell of aspect-ratio ?=4 filled with water. Particle image velocimetry (PIV), laser Doppler anemometry (LDA), and liquid crystal thermography (LCT) measurements are used to obtain velocity and temperature information. The first part of this dissertation describes an investigation of the large-scale circulation (LSC) in Rayleigh-Bénard convection. In the second part, a study of the boundary layers in Rayleigh-Bénard convection is described. The last part reports on the control and enhancement of the heat transfer in Rayleigh-Bénard convection using an additional electromagnetic body force.Multi-Scale PhysicsApplied Science