Exogenous application of platelet-leukocyte gel during open subacromial decompression contributes to improved patient outcome

Background: Platelet-leukocyte gel (PLG) is being used during various surgical procedures in an attempt to enhance the healing process. We studied the effects of PLG on postoperative recovery of patients undergoing open subacromial decompression (OSD). Methods: PLG was produced from platelet-leukocyte-rich plasma (P-LRP), prepared from a unit of whole blood. Forty patients were included in the study. Self-assessed evaluations, using the American Shoulder and Elbow Surgeons scoring system of activities of daily living (ADL), joint instability, pain levels, pain medications, and clinical evaluations for range of motion were conducted. Results: Platelet and leukocyte counts were significantly increased in the P-LRP compared to baseline counts. Treated patients demonstrated decreased visual analog scales for pain and used significantly less pain medication, had an improved range of motion during passive forward elevation, external rotation, external rotation with arm at 90 degrees abduction, internal rotation, and cross body adduction compared to control patients (p < 0.001). No differences in the instability score were observed between the groups. Furthermore, treated patients performed more ADL (p < 0.05). Conclusion: In the PLG-treated group, recovery was faster and patients returned earlier to daily activities and also took less pain medication than control subjects

Teacher's corner : evaluating informative hypotheses using the Bayes factor in structural equation models

This Teacher's Corner paper introduces Bayesian evaluation of informative hypotheses for structural equation models, using the free open-source R packages bain, for Bayesian informative hypothesis testing, and lavaan, a widely used SEM package. The introduction provides a brief non-technical explanation of informative hypotheses, the statistical underpinnings of Bayesian hypothesis evaluation, and the bain algorithm. Three tutorial examples demonstrate informative hypothesis evaluation in the context of common types of structural equation models: 1) confirmatory factor analysis, 2) latent variable regression, and 3) multiple group analysis. We discuss hypothesis formulation, the interpretation of Bayes factors and posterior model probabilities, and sensitivity analysis

What Are You Trying to Say? The Interface as an Integral Element of Argument

Graphical interfaces to digital scholarly editions are usually regarded as disconnected from the content of the edition, enough so that an argument has developed against the use of interfaces at all. We argue in this paper that the indifference and even hostility to interfaces is caused by a widespread incomprehension of their argumentative utility. In a pair of case studies of published digital editions, we conduct a detailed examination of the argument their interface makes, and compare these interface rhetorics with the stated intentions of the editors, exposing a number of contradictions between ‘word’ and ‘deed’ in the interface designs. We end by advocating for an explicit consideration of the semiotic significance of the elements of a user interface: that editors reflect on what aspect of the argument their interface expresses, and how that is adding, or perhaps subtracting, from the points they wish to make

Protein-protein modelling using cryo-EM restraints

The recent improvements in cryo-electron microscopy (cryo-EM) in the past few years are now allowing to observe molecular complexes at atomic resolution. As a consequence, numerous structures derived from cryo-EM are now available in the Protein Data Bank. However, if for some complexes atomic resolution is reached, this is not true for all. This is also the case in cryo-electron tomography where the achievable resolution is still limited. Furthermore the resolution in a cryo-EM map is not a constant, with often outer regions being of lower resolution, possibly linked to conformational variability. Although those low to medium resolution EM maps (or regions thereof) cannot directly provide atomic structure of large molecular complexes, they provide valuable information to model the individual components and their assembly into them. Most approaches for this kind of modelling are performing rigid fitting of the individual components into the EM density map. While this would appear an obvious option, they ignore key aspects of molecular recognition, the energetics and flexibility of the interfaces. Moreover, these often restricts the modelling to a unique source of data, the EM density map. In this chapter, we describe a protocol where an EM map is used as restraint in HADDOCK to guide the modelling process.Comment: 28 pages including 7 figure