Initial tests and operation of a 110 GHz, 1 MW gyrotron with evacuated waveguide system on the DIII-D tokamak

A gyrotron producing nominally 1 MW at 110 GHz has been installed at the DIII-D tokamak and operated in a program of initial tests with a windowless evacuated transmission line. The alignment and first test operation were performed in an air environment at atmospheric pressure. Under these conditions, the tube produced rf output in excess of 800 kW for pulse lengths greater than 10 msec and power near 500 kW for pulse lengths of about 100 msec into a free space dummy load. The gyrotron was operated into evacuated corrugated waveguide in the full power parameter regime for pulse lengths of up to 500 msec injecting greater than 0.5 MW into DIII-D for a preliminary series of experiments. Generated powers greater than 900 kW were achieved. A parasitic oscillation at various frequencies between 20 and 100 MHz, which was generated during the pulsing of the gyrotron electron beam, was suppressed somewhat by a capacitive filter attached to the gyrotron itself. Addition of a magnetic shield intended to alter the magnetic field geometry below the cathode eliminated internal tube sparks. Rework of the external power and interlock circuitry to improve the immunity to electromagnetic interference was also done in parallel so that the fast interlock circuitry could be used. The latest results of the test program, the design of the free space load and other test hardware, and the transmission line will be presented

Stability of High Voltage Modulators for Nonlinear Loads

OAK-B135 Gyrotrons have a nonlinear voltage--current characteristic such that the small signal or ac impedance changes as operational voltage and currents are reached. The ac impedance determines the stability of a voltage or current control system. this can become particularly challenging when several gyrotron are connected in parallel to a single modulator. With all gyrotrons hooked to a common ground, large current loops can be generated as well as non-canceling currents in individual coaxial lines. These inequalities can provide the required feedback impulse to start an oscillation condition in the power system for the tubes. Recent operation of two CPI 110 GHz gyrotrons in the MN class from a single modulator on DIII-D has shown instability in the power system. An oscillation in the drive current occurs at various points in the ramp up and flat top portions of the 80 kV voltage pulse with each tube drawing 40 A at full voltage. Efforts to stabilize these instabilities are presented along with some modeling and examination of the issues for gyrotron modulators

Status of the DIII-D 110 GHz ECH system

The DIII-D program is presently commissioning the first NM gyrotron of a planned 3 MW, I 10 GHz electron cyclotron heating (ECH) system for off-axis electron heating and current drive. Advanced tokamak (AT) research in DIII-D and other tokamaks requires the ability to control the current density profile. ECH offers the ability to localize the heating and driven current in a controllable manner and is not dependent upon, the local plasma conditions, so it appears to be an ideal tool for AT research. The planned rf sources for the DIII-D system are I MW state-of-the-art internal mode-converter gyrotrons, with one gyrotron being manufactured by GYCOM, a Russian company, and two gyrotrons being manufactured by CPI (formerly Varian). The GYCOM gyrotron has been tested at the factory to 960 kW, 2 seconds and has been shipped to GA where it is now undergoing initial checkout and testing. The first CPI gyrotron has been assembled and factory tested to 530 kW, 2 seconds and 350 1352 kW, 10 seconds. Both the GYCOM and CPI gyrotrons are limited in pulse length at full power by thermal limits on the output window. The second CPI gyrotron is expected to be ready for testing in April 1996. This paper will report on the initial experiences of using the GYCOM I MW, 110 GHz internal mode- converter gyrotron, at General Atomics, and the observed effects the ECRH power has on the DIII-D plasma

Removable silicon insertion stiffeners for neural probes using polyethylene glycol as a biodissolvable adhesive

Abstract not provide

Practical Experiences with the 6 Gyrotron System on the DIII-D Tokamak

OAK-B135 The gyrotron installation on the DIII-D tokamak now comprises six 110 GHz gyrotrons in the 1 MW class, three manufactured by CPI[1] and three by Gycom [2]. Two tetrode rectifier/modulator/regulator power supplies were constructed to provide power for the CPI gyrotrons. A second system uses three mod/reg tetrodes connected in parallel, which are fed by a dual parallel tetrode mod/reg to power the Gycom tubes. the windowless evacuated transmission lines are up to 100 m in length, with 80% transmission efficiency. Engineering solutions were developed in specific problematic areas encountered in the development of this complex system, including: Gyrotron instability; high voltage circuit instability; gyrotron conditioning; rf beam forming and coupling to waveguide; output window vacuum seals; material control; launcher mechanics and diagnosis; polarizer mechanics; dummy loads; power measurements; polarization measurements; cooling; calorimetry; and operating controls. The system is in routine operation in support of tokamak experiments, with peak generated power of about 5 MW at 2 s. pulse length and about 3 MW for 5 s. pulses. This presentation focuses on practical lessons learned in the development and operation of these systems

Intracellular polyphosphate length characterization in polyphosphate accumulating microorganisms (PAOs): Implications in PAO phenotypic diversity and enhanced biological phosphorus removal performance

Polyphosphate (polyP) accumulating organisms (PAOs) are the key agent to perform enhanced biological phosphorus removal (EBPR) activity, and intracellular polyP plays a key role in this process. Potential associations between EBPR performance and the polyP structure have been suggested, but are yet to be extensively investigated, mainly due to the lack of established methods for polyP characterization in the EBPR system. In this study, we explored and demonstrated that single-cell Raman spectroscopy (SCRS) can be employed for characterizing intracellular polyPs of PAOs in complex environmental samples such as EBPR systems. The results, for the first time, revealed distinct distribution patterns of polyP length (as Raman peak position) in PAOs in lab-scale EBPR reactors that were dominated with different PAO types, as well as among different full-scale EBPR systems with varying configurations. Furthermore, SCRS revealed distinctive polyP composition/features among PAO phenotypic sub-groups, which are likely associated with phylogenetic and/or phenotypic diversity in EBPR communities, highlighting the possible resolving power of SCRS at the microdiversity level. To validate the observed polyP length variations via SCRS, we also performed and compared bulk polyP length characteristics in EBPR biomass using conventional polyacrylamide gel electrophoresis (PAGE) and solution 31P nuclear magnetic resonance (31P-NMR) methods. The results are consistent with the SCRS findings and confirmed the variations in the polyP lengths among different EBPR systems. Compared to conventional methods, SCRS exhibited advantages as compared to conventional methods, including the ability to characterize in situ the intracellular polyPs at subcellular resolution in a label-free and non-destructive way, and the capability to capture subtle and detailed biochemical fingerprints of cells for phenotypic classification. SCRS also has recognized limitations in comparison with 31P-NMR and PAGE, such as the inability to quantitatively detect the average polyP chain length and its distribution. The results provided initial evidence for the potential of SCRS-enabled polyP characterization as an alternative and complementary microbial community phenotyping method to facilitate the phenotype-function (performance) relationship deduction in EBPR systems

Insect Eggs Can Enhance Wound Response in Plants: A Study System of Tomato Solanum lycopersicum L. and Helicoverpa zea Boddie

Insect oviposition on plants frequently precedes herbivory. Accumulating evidence indicates that plants recognize insect oviposition and elicit direct or indirect defenses to reduce the pressure of future herbivory. Most of the oviposition-triggered plant defenses described thus far remove eggs or keep them away from the host plant or their desirable feeding sites. Here, we report induction of antiherbivore defense by insect oviposition which targets newly hatched larvae, not the eggs, in the system of tomato Solanum lycopersicum L., and tomato fruitworm moth Helicoverpa zea Boddie. When tomato plants were oviposited by H. zea moths, pin2, a highly inducible gene encoding protease inhibitor2, which is a representative defense protein against herbivorous arthropods, was expressed at significantly higher level at the oviposition site than surrounding tissues, and expression decreased with distance away from the site of oviposition. Moreover, more eggs resulted in higher pin2 expression in leaves, and both fertilized and unfertilized eggs induced pin2 expression. Notably, when quantified daily following deposition of eggs, pin2 expression at the oviposition site was highest just before the emergence of larvae. Furthermore, H. zea oviposition primed the wound-induced increase of pin2 transcription and a burst of jasmonic acid (JA); tomato plants previously exposed to H. zea oviposition showed significantly stronger induction of pin2 and higher production of JA upon subsequent simulated herbivory than without oviposition. Our results suggest that tomato plants recognize H. zea oviposition as a signal of impending future herbivory and induce defenses to prepare for this herbivory by newly hatched neonate larvae

The use of common bean (Phaseolus vulgaris ) traditional varieties and their mixtures with commercial varieties to manage bean fly (Ophiomyia spp .) infestations in Uganda

The bean fly (Ophiomyia spp.) is considered the most economically damaging field insect pest of common beans in Uganda. Despite the use of existing pest management approaches, reported damage has remained high. Forty-eight traditional and improved common bean varieties currently grown in farmers’ fields were evaluated for resistance against bean fly. Data on bean fly incidence, severity and root damage from bean stem maggot were collected. Generalized linear mixed model (GLMM) revealed significant resistance to bean fly in the Ugandan traditional varieties. A popular resistant traditional variety and a popular susceptible commercial variety were selected from the 48 varieties and evaluated in pure and mixed stands. The incidence of bean fly infestation on both varieties in mixtures with different arrangements (systematic random versus rows), and different proportions within each of the two arrangements, was measured and analysed using GLMMs. The proportion of resistant varieties in a mixture and the arrangement type significantly decreased bean fly damage compared to pure stands, with the highest decrease in damage registered in the systematic random mixture with at least 50 % of resistant variety. The highest reduction in root damage, obvious 21 days after planting, was found in systematic random mixtures with at least 50 % of the resistant variety. Small holder farmers in East Africa and elsewhere in the world have local preferences for growing bean varieties in genetic mixtures. These mixtures can be enhanced by the use of resistant varieties in the mixtures to reduce bean fly damage on susceptible popular varieties

Salivary Glucose Oxidase from Caterpillars Mediates the Induction of Rapid and Delayed-Induced Defenses in the Tomato Plant

Caterpillars produce oral secretions that may serve as cues to elicit plant defenses, but in other cases these secretions have been shown to suppress plant defenses. Ongoing work in our laboratory has focused on the salivary secretions of the tomato fruitworm, Helicoverpa zea. In previous studies we have shown that saliva and its principal component glucose oxidase acts as an effector by suppressing defenses in tobacco. In this current study, we report that saliva elicits a burst of jasmonic acid (JA) and the induction of late responding defense genes such as proteinase inhibitor 2 (Pin2). Transcripts encoding early response genes associated with the JA pathway were not affected by saliva. We also observed a delayed response to saliva with increased densities of Type VI glandular trichomes in newly emerged leaves. Proteomic analysis of saliva revealed glucose oxidase (GOX) was the most abundant protein identified and we confirmed that it plays a primary role in the induction of defenses in tomato. These results suggest that the recognition of GOX in tomato may represent a case for effector-triggered immunity. Examination of saliva from other caterpillar species indicates that saliva from the noctuids Spodoptera exigua and Heliothis virescens also induced Pin2 transcripts