Gynandromorphs in the genera Ophiogomphus Selys, 1854 and Ischnura Charpentier, 1840 (Odonata: Gomphidae,Coenagrionidae)

A gynandromorph of Ophiogomphus smithi Tennessen and Vogt from Wisconsin and a gynandromorph of Ischnura hastata (Say) from Alabama are described. The specimens appear to be bilateral in that they display mostly left/right separation of male and female characters

First Records of \u3ci\u3eGomphaeschna Furcillata\u3c/i\u3e and \u3ci\u3eAnax Longipes\u3c/i\u3e in Wisconsin (Odonata: Aeshnidae)

(excerpt) The two new records of Odonata reported here for Wisconsin are north- western range extensions for each species. The genus Gomphaeschna Selys contains two species, antilope (Hagen) and furcillata (Say), the distributions of which are limited to eastern North America. Gomphaeschna antilope ranges from New Jersey west to Ohio and south to Louisiana and Florida (Needham and Westfall 1955). Gomphaeschna furcillata is similarly distributed in the south, but ranges further north, to Nova Scotia and Maine eastward, and to Michigan westward

New Distribution Records for \u3ci\u3eOphiogomphus Howei\u3c/i\u3e (Odonata: Gomphidae)

New distribution records establish Ophiogomphus howei as a resident species in Wisconsin. Kentucky, Maine, and Minnesota are added as new state records for this seldom-encountered gomphid dragonfly. Larval habitats appear to be undisturbed rivers 10-200 m wide, with sand and gravel sub-strates

How Did E. M. Walker Measure the Length of the Labium of Nymphs of \u3ci\u3eAeshna\u3c/i\u3e and \u3ci\u3eRhionaeschna\u3c/i\u3e (Odonata: Aeshnidae)?

The exhaustive studies of nymphs of Aeshna Fabricius and Rhionaeschna Förster by E. M. Walker (1912-1958) have long guided the taxonomy of these groups and formed the basis for keys still in use today. However, uncertainty about how he measured the length of the labium, including the varied terminology he used over the duration of his career concerning this structure, has led to confusion about application of his taxonomic recommendations. We recalculated ratios of the maximum width/length [W(max)/L] by measuring the illustration dimensions of folded labia and prementums in publications throughout his career and compared these data with the ratios he stated in those publications and with ratios derived from measurements of specimens in our collections. Our results show that from 1912 to 1941, Walker restricted length measurement to the prementum proper (which he called the “mentum of the labium”), exclusive of the ventrally visible portion of the postmental hinge. However, in 1941 he reported ratios from length measurements done two ways, excluding the postmental hinge in his description of the nymph of A. verticalis Hagen, but including the hinge in his description of the nymph of A. septentrionalis Burmeister (Whitehouse 1941). In Walker’s most recent and influential work (1958), he included the postmental hinge in labium length measurements of nine species, but restricted length measurements to the prementum for five others. He was consistent with the use of terms, using both “folded labium” by which he meant the prementum plus the postmental hinge, and “prementum” by which he meant only that structure. However, Walker’s descriptions of the labium in his latest work are buried in long, frequently punctuated sentences that for most species include the terms “folded labium” and “prementum” in the same sentence, so careful reading is required to know which term is intended in the width/length ratio. Width/length ratios we each calculated independently were invariably similar for a given species and were usually similar to Walker’s stated ratio for that species. These similarities affirm our conclusion that while labium measurements must be done with care, they are closely repeatable among workers and will consistently lead to correct determinations in properly designed couplets of dichotomous keys to these genera. We recommend measuring the length of the prementum proper in future studies of these genera when labium ratios are calculated because we found less variability in those cases than when the measurements included the postmental hinge. An approximate conversion between the two methods of calculating W(max)/L ratios can be made as follows: ratio calculated when the length of the prementum excluding the postmental hinge is used x 0.88 is approximately equal to the ratio when the postmental hinge is included for species of Aeshna and Rhionaeschna in North America

A Hybrid Male in the Genus Ophiogomphus (Odonata: Gomphidae)

A gomphid male from west-central Wisconsin (Eau Claire County, North Fork Eau Claire River, 11 June 1994, K. J. Tennessen leg) with characters that are intermediate between Ophiogomphus carolus Needham, 1897 and Ophiogomphus rupinsulensis (Walsh), 1862 is described and illustrated. The specimen appears to be a hybrid based on intermediate character states of 1) color pattern (slightly closer to O. carolus), 2) hamule morphology (shaped slightly more like those of O. carolus), and 3) anal appendage morphology (slightly more like those of O. rupinsulensis)

A Hybrid Male in the Genus Ophiogomphus (Odonata: Gomphidae)

A gomphid male from west-central Wisconsin (Eau Claire County, North Fork Eau Claire River, 11 June 1994, K. J. Tennessen leg) with characters that are intermediate between Ophiogomphus carolus Needham, 1897 and Ophiogomphus rupinsulensis (Walsh), 1862 is described and illustrated. The specimen appears to be a hybrid based on intermediate character states of 1) color pattern (slightly closer to O. carolus), 2) hamule morphology (shaped slightly more like those of O. carolus), and 3) anal appendage morphology (slightly more like those of O. rupinsulensis)

Efficacy of Morphological Characters for Distinguishing Nymphs of \u3ci\u3eEpitheca Cynosura\u3c/i\u3e and \u3ci\u3eEpitheca Spinigera\u3c/i\u3e (Odonata: Corduliidae) in Wisconsin

Attempts to distinguish exuviae and last-instar nymphs of Epitheca cynosura (Say) and Epitheca spinigera (Selys) (Odonata: Corduliidae) using lateral spine characters have proven to be unreliable, and recent use of setae counts on only one side of the prementum or one labial palp have led to confusion because these structures often hold unequal numbers of setae on the two sides of the same specimen. Based on exuviae of 67 reared E. cynosura and 55 reared E. spinigera from lakes throughout Wisconsin, we tested the efficacy of previously used character states for distinguishing these species and searched for new characters to improve the reliability of regional keys. The most reliable diagnostic character was the combined number of setae on both sides of the prementum and on both labial palps (≤ 35 – E. cynosura; ≥ 36 – E. spinigera), which correctly determined 96% of our specimens. For the small percentage of specimens that lie in the region of overlap in total setae number, we found that total exuviae length, cerci ÷ epiproct ratios of females, tubercle distance ÷ epiproct ratios of males, and the shape of the dorsal hook on segment 8 could be used to strengthen determinations

The Puzzling Presence of Lestes australis (Odonata: Lestidae) in Wisconsin - Does This Species Migrate?

Lestes disjunctus australis Walker, 1952 was named as a subspecies of Lestes disjunctus Selys, 1862. In recent decades it has been considered deserving of full species status by most specialists. The core of its eastern North American range is south of Wisconsin, but during April through June of some years, mature individuals, and occasionally reproductive behavior, are observed at shallow ponds and wetlands mostly in the southern half of the state. Since first recorded in Wisconsin in 2002, it has been detected in 13 of Wisconsin’s 72 counties. However, there has been no unequivocal documentation of successful reproduction in the state. Various possibilities regarding long-range dispersal or facultative migration of this species and other species of Zygoptera are discussed

Efficacy of Morphological Characters for Distinguishing Nymphs of \u3ci\u3eEpitheca Cynosura\u3c/i\u3e and \u3ci\u3eEpitheca Spinigera\u3c/i\u3e (Odonata: Corduliidae) in Wisconsin

Attempts to distinguish exuviae and last-instar nymphs of Epitheca cynosura (Say) and Epitheca spinigera (Selys) (Odonata: Corduliidae) using lateral spine characters have proven to be unreliable, and recent use of setae counts on only one side of the prementum or one labial palp have led to confusion because these structures often hold unequal numbers of setae on the two sides of the same specimen. Based on exuviae of 67 reared E. cynosura and 55 reared E. spinigera from lakes throughout Wisconsin, we tested the efficacy of previously used character states for distinguishing these species and searched for new characters to improve the reliability of regional keys. The most reliable diagnostic character was the combined number of setae on both sides of the prementum and on both labial palps (≤ 35 – E. cynosura; ≥ 36 – E. spinigera), which correctly determined 96% of our specimens. For the small percentage of specimens that lie in the region of overlap in total setae number, we found that total exuviae length, cerci ÷ epiproct ratios of females, tubercle distance ÷ epiproct ratios of males, and the shape of the dorsal hook on segment 8 could be used to strengthen determinations

Balancing Selection at a Frog Antimicrobial Peptide Locus: Fluctuating Immune Effector Alleles?

Balancing selection is common on many defense genes, but it has rarely been reported for immune effector proteins such as antimicrobial peptides (AMPs). We describe genetic diversity at a brevinin-1 AMP locus in three species of leopard frogs (Rana pipiens, Rana blairi, and Rana palustris). Several highly divergent allelic lineages are segregating at this locus. That this unusual pattern results from balancing selection is demonstrated by multiple lines of evidence, including a ratio of nonsynonymous/synonymous polymorphism significantly higher than 1, the ZnS test, incongruence between the number of segregating sites and haplotype diversity, and significant Tajima's D values. Our data are more consistent with a model of fluctuating selection in which alleles change frequencies over time than with a model of stable balancing selection such as overdominance. Evidence for fluctuating selection includes skewed allele frequencies, low levels of synonymous variation, nonneutral values of Tajima's D within allelic lineages, an inverse relationship between the frequency of an allelic lineage and its degree of polymorphism, and divergent allele frequencies among populations. AMP loci could be important sites of adaptive genetic diversity, with consequences for host–pathogen coevolution and the ability of species to resist disease epidemics