PROTECTION OF EMPLOYEES IN OUR LEGAL SYSTEM WITH REFERENCE TO AGROCOMPLEX

This study deals with the always present question of the safety and protection of employees which is especially popular in the conditions of transitional changes that our society is going through and in accordance with it our economic system, in which agrocomplex has a significant place. There is an emphasis on general conditions in which employees work and on normative solutions of our legal system which follow the norms contained in the law of the EU and in comparative legal system as well

PROTECTION OF EMPLOYEES IN OUR LEGAL SYSTEM WITH REFERENCE TO AGROCOMPLEX

This study deals with the always present question of the safety and protection of employees which is especially popular in the conditions of transitional changes that our society is going through and in accordance with it our economic system, in which agrocomplex has a significant place. There is an emphasis on general conditions in which employees work and on normative solutions of our legal system which follow the norms contained in the law of the EU and in comparative legal system as well

Phone call duration with City Department of Urgent medical care in the chest pain case, before medical assistance is sent

Introduction: Chest pain or discomfort is a potentially life threatening medical state. Every delay from symptoms onset to delivering medical assistance is of utmost importance. Avoiding prehospital delay is crucial. Aim: Establish phone call duration with an emergency operator in the cases of chest pain. Method: Electronic switchboard database from the City Department of Urgent medical care was used. We analysed accepted calls from April 1st to December 1st, 2009. Measured time starts with emergency operator call acceptance and ends with forwarding the call to the dispacher. Results: We analysed 2469 phone calls, with minimal duration of 82 seconds, maximal duration of 385 seconds and mean duration of 182 seconds; 50% of phone calls were finished after 167 seconds and 90% of all calls after 288 seconds. Discussion: Recommended time in different Emergency care facilities varies from 60s to 90s to 120s. One of the standards recommends that 99% of phone calls should be finished within 90s, which is much shorter then our research showed. Conclusion: Our research showed rather long mean and maximal phone call durations. Time frame must be defined as a rule, and although it may not always be able to act within, it is something which should be defined and aspired to

Effects of substrates and temperatures on Cynara cardunculus L. seed germination

The aim of this study was to determine the effects of substrates and temperatures on Cynara cardunculus seed germination. The tests encompassed the following substrates - filter paper, sand and compost. The follow-ing temperature regimes were applied on each substrate used for germination tests: 20/30°C (T 1), 20°C (T 2) and 25°C after 10-day chilling at 10°C (T 3). The analysis of variance showed that there were statistically significant differences between the first counts and the total seed germination, depending on the substrate type, tempera-ture regime and medium × temperature regime interaction The highest germination was detected on filter paper, whereas the lowest was on sand. Furthermore, the highest germination was determined at a constant temperature of 20°C (T 2), while the lowest, at a temperature regime of 25°C after 10-day chilling at 10°C (T 3)

Identification and characterization of a novel flavin-containing spermine oxidase of mammalian cell origin.

During polyamine catabolism, spermine and spermidine are first acetylated by spermidine/spermine N(1)-acetyltransferase (SSAT) and subsequently oxidized by polyamine oxidase (PAO) to produce spermidine and putrescine, respectively. In attempting to clone the PAO involved in this back-conversion pathway, we encountered an oxidase that preferentially cleaves spermine in the absence of prior acetylation by SSAT. A BLAST search using maize PAO sequences identified homologous mammalian cDNAs derived from human hepatoma and mouse mammary carcinoma: the encoded proteins differed by 20 amino acids. When either cDNA was transiently transfected into HEK-293 cells, intracellular spermine pools decreased by 75% while spermidine and N (1)-acetylspermidine pools increased, suggesting that spermine was selectively and directly oxidized by the enzyme. Substrate specificity using lysates of oxidase-transfected HEK-293 cells revealed that the newly identified oxidase strongly favoured spermine over N (1)-acetylspermine and that it failed to act on N (1)-acetylspermidine, spermidine or the preferred PAO substrate, N (1), N (12)-diacetylspermine. The PAO inhibitor, MDL-72,527, only partially blocked oxidation of spermine while a previously reported PAO substrate, N (1)-( n -octanesulphonyl)spermine, potently inhibited the reaction. Overall, the data indicate that the enzyme represents a novel mammalian oxidase which, on the basis of substrate specificity, we have designated spermine oxidase in order to distinguish it from the PAO involved in polyamine back-conversion. The identification of an enzyme capable of directly oxidizing spermine to spermidine has important implications for understanding polyamine homoeostasis and for interpreting metabolic and cellular responses to clinically relevant polyamine analogues and inhibitors

Radioactivity and measurements of sediment deposition rate of the Drenova reservoir (B&H)

This work presents the first estimate of the radioactivity and sediment deposition rate of the Drenova reservoir. The radioactivity and sedimentation rate were computed applying the 210Pb and 137Cs methods. Samples of 210Pb and 137Cs were taken from four boreholes drilled in the Drenova reservoir in June 2010. Vertical distribution of the natural and artificial radionuclides in four boreholes was examined using a gamma spectrometry measurement with HpGe detectors, Gamma X type (10 keV-3 MeV). Activities ranging from 122-8 Bq/kg were found for 210Pb, and from 140-0.8 Bq/kg for 137Cs. The sedimentation rate in the Drenova reservoir varied from 1.96 to 2.90 cm per year for unsupported 210Pb and 0.47 to 5.33 cm per year for 137Cs

MHC-restricted fratricide of human lymphocytes expressing survivin-specific transgenic T cell receptors

The apoptosis inhibitor protein survivin is overexpressed in many tumors, making it a candidate target molecule for various forms of immunotherapy. To explore survivin as a target antigen for adoptive T cell therapy using lymphocytes expressing survivin-specific transgenic T cell receptors (Tg-TCRs), we isolated HLA-A2–allorestricted survivin-specific T cells with high functional avidity. Lymphocytes expressing Tg-TCRs were derived from these T cells and specifically recognized HLA-A2+ survivin+ tumor cells. Surprisingly, HLA-A2+ but not HLA-A2– lymphocytes expressing Tg-TCRs underwent extensive apoptosis over time. This demise was caused by HLA-A2–restricted fratricide that occurred due to survivin expression in lymphocytes, which created ligands for Tg-TCR recognition. Therefore, survivin-specific TCR gene therapy would be limited to application in HLA-A2–mismatched stem cell transplantation. We also noted that lymphocytes that expressed survivin-specific Tg-TCRs killed T cell clones of various specificities derived from HLA-A2+ but not HLA-A2– donors. These results raise a general question regarding the development of cancer vaccines that target proteins that are also expressed in activated lymphocytes, since induction of high-avidity T cells that expand in lymph nodes following vaccination or later accumulate at tumor sites might limit themselves by self-MHC–restricted fratricide while at the same time inadvertently eliminating neighboring T cells of other specificities