Comparison between measured and computed magnetic flux density distribution of simulated transformer core joints assembled from grain oriented and non-oriented electrical steel

The flux distribution in an overlapped linear joint constructed in the central region of an Epstein Square was studied experimentally and results compared with those obtained using a computational magnetic field solver. High permeability grain-oriented (GO) and low permeability non-oriented (NO) electrical steels were compared at a nominal core flux density of 1.60 T at 50 Hz. It was found that the experimental results only agreed well at flux densities at which the reluctance of different paths of the flux are similar. Also it was revealed that the flux becomes more uniform when the working point of the electrical steel is close to the knee point of the B-H curve of the steel

Doxorubicin Removal from Water using Acid-treated Activated Carbon, Multi-walled Carbon Nanotubes, and Montmorillonite

Medical wastewater is a significant contributor to environmental pollution, posing severe risks to both human health and the environment. To resolve this challenge, the removal of anti-cancer drugs from medical wastewater has been considered. This study investigated the removal of doxorubicin, an effective anti-cancer drug, from an aqueous solution using three types of adsorbents: activated carbon, multi-walled carbon nanotube, and montmorillonite. Our findings revealed that carbon nanotubes exhibited superior performance in doxorubicin removal from water compared to the other two adsorbents. Specifically, the maximum adsorption capacity of doxorubicin with an initial concentration of 50 mg L–1 on the carbon nanotube reached 500 mg g–1. In addition, surface modification of the adsorbents with acid resulted in a 15 % and 41 % increase in adsorption capacity, and an 85 % and 67 % reduction in equilibrium time for carbon nanotube and montmorillonite, respectively. The increasing pH proved to enhance the adsorption efficiency of carbon nanotubes and activated carbon, with the best performance achieved at solution pH of 10 and 8 for MWCNTs and AC, respectively

Image_1_The expression and role of the Lem-D proteins Ankle2, Emerin, Lemd2, and TMPO in triple-negative breast cancer cell growth.pdf

BackgroundTriple-negative breast cancer (TNBC) is a sub-classification of breast carcinomas, which leads to poor survival outcomes for patients. TNBCs do not possess the hormone receptors that are frequently targeted as a therapeutic in other cancer subtypes and, therefore, chemotherapy remains the standard treatment for TNBC. Nuclear envelope proteins are frequently dysregulated in cancer cells, supporting their potential as novel cancer therapy targets. The Lem-domain (Lem-D) (LAP2, Emerin, MAN1 domain, and Lem-D) proteins are a family of inner nuclear membrane proteins, which share a ~45-residue Lem-D. The Lem-D proteins, including Ankle2, Lemd2, TMPO, and Emerin, have been shown to be associated with many of the hallmarks of cancer. This study aimed to define the association between the Lem-D proteins and TNBC and determine whether these proteins could be promising therapeutic targets.MethodsGENT2, TCGA, and KM plotter were utilized to investigate the expression and prognostic implications of several Lem-D proteins: Ankle2, TMPO, Emerin, and Lemd2 in publicly available breast cancer patient data. Immunoblotting and immunofluorescent analysis of immortalized non-cancerous breast cells and a panel of TNBC cells were utilized to establish whether protein expression of the Lem-D proteins was significantly altered in TNBC. SiRNA was used to decrease individual Lem-D protein expression, and functional assays, including proliferation assays and apoptosis assays, were conducted.ResultsThe Lem-D proteins were generally overexpressed in TNBC patient samples at the mRNA level and showed variable expression at the protein level in TNBC cell lysates. Similarly, protein levels were generally negatively correlated with patient survival outcomes. siRNA-mediated depletion of the individual Lem-D proteins in TNBC cells induced aberrant nuclear morphology, decreased proliferation, and induced cell death. However, minimal effects on nuclear morphology or cell viability were observed following Lem-D depletion in non-cancerous MCF10A cells.ConclusionThere is evidence to suggest that Ankle2, TMPO, Emerin, and Lemd2 expressions are correlated with breast cancer patient outcomes, but larger patient sample numbers are required to confirm this. siRNA-mediated depletion of these proteins was shown to specifically impair TNBC cell growth, suggesting that the Lem-D proteins may be a specific anti-cancer target.</p