115 research outputs found
Real-Time Human Motion Capture with Multiple Depth Cameras
Commonly used human motion capture systems require intrusive attachment of
markers that are visually tracked with multiple cameras. In this work we
present an efficient and inexpensive solution to markerless motion capture
using only a few Kinect sensors. Unlike the previous work on 3d pose estimation
using a single depth camera, we relax constraints on the camera location and do
not assume a co-operative user. We apply recent image segmentation techniques
to depth images and use curriculum learning to train our system on purely
synthetic data. Our method accurately localizes body parts without requiring an
explicit shape model. The body joint locations are then recovered by combining
evidence from multiple views in real-time. We also introduce a dataset of ~6
million synthetic depth frames for pose estimation from multiple cameras and
exceed state-of-the-art results on the Berkeley MHAD dataset.Comment: Accepted to computer robot vision 201
Play and Learn: Using Video Games to Train Computer Vision Models
Video games are a compelling source of annotated data as they can readily
provide fine-grained groundtruth for diverse tasks. However, it is not clear
whether the synthetically generated data has enough resemblance to the
real-world images to improve the performance of computer vision models in
practice. We present experiments assessing the effectiveness on real-world data
of systems trained on synthetic RGB images that are extracted from a video
game. We collected over 60000 synthetic samples from a modern video game with
similar conditions to the real-world CamVid and Cityscapes datasets. We provide
several experiments to demonstrate that the synthetically generated RGB images
can be used to improve the performance of deep neural networks on both image
segmentation and depth estimation. These results show that a convolutional
network trained on synthetic data achieves a similar test error to a network
that is trained on real-world data for dense image classification. Furthermore,
the synthetically generated RGB images can provide similar or better results
compared to the real-world datasets if a simple domain adaptation technique is
applied. Our results suggest that collaboration with game developers for an
accessible interface to gather data is potentially a fruitful direction for
future work in computer vision.Comment: To appear in the British Machine Vision Conference (BMVC), September
2016. -v2: fixed a typo in the reference
Data supporting development and validation of liquid chromatography tandem mass spectrometry method for the quantitative determination of bile acids in feces
Measuring bile acids in feces has an important role in disease prevention, diagnosis, treatment, and can be considered a measure of health status. Therefore, the primary aim was to develop a sensitive, robust, and high throughput liquid chromatography tandem mass spectrometry method with minimal sample preparation for quantitative determination of bile acids in human feces applicable to large cohorts. Due to the chemical diversity of bile acids, their wide concentration range in feces, and the complexity of feces itself, developing a sensitive and selective analytical method for bile acids is challenging. A simple extraction method using methanol suitable for subsequent quantification by liquid chromatography tandem mass spectrometry has been reported in, âExtraction and quantitative determination of bile acids in fecesâ [1]. The data highlight the importance of optimization of the extraction procedure and the stability of the bile acids in feces post-extraction and prior to analysis and after several freeze-thaw cycles
Development of a food composition database for the estimation of dietary s-methyl cysteine sulfoxide from vegetables
A food composition database estimating S-methyl cysteine sulfoxide (SMCSO) was created following a systematic literature search. SMCSO data (705 entries) from 19 vegetables were summarised: brassicas (n = 10) and alliums (n = 9). The highest SMCSO in brassicas was reported in Brussels sprouts (median [range]: 318 [68â420] mg/100 g fresh weight (FW)) whilst the lowest was in radish (19 [4â45] mg/100 g FW). Brussels sprouts were almost twice as concentrated in SMCSO as cauliflower, followed by cabbage, kale, broccoli, kohlrabi, swede, Chinese cabbage, and turnips. The alliums highest in SMCSO were Chinese chives (271 [185â413] mg/100 g FW) followed by rakkyo and garlic, with substantially less found in shallots, onion, and leek. Literature reporting SMCSO content in food is sparse. Further research is required to quantify SMCSO in commercially available vegetables and other foods, in order to update and expand this database for application to large populations and future intervention studies
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A Peroxidase Peroxiredoxin 1-Specific Redox Regulation of the Novel FOXO3 microRNA Target let-7
Abstract Precision in redox signaling is attained through posttranslational protein modifications such as oxidation of protein thiols. The peroxidase peroxiredoxin 1 (PRDX1) regulates signal transduction through changes in thiol oxidation of its cysteines. We demonstrate here that PRDX1 is a binding partner for the tumor suppressive transcription factor FOXO3 that directly regulates the FOXO3 stress response. Heightened oxidative stress evokes formation of disulfide-bound heterotrimers linking dimeric PRDX1 to monomeric FOXO3. Absence of PRDX1 enhances FOXO3 nuclear localization and transcription that are dependent on the presence of Cys31 or Cys150 within FOXO3. Notably, FOXO3-T32 phosphorylation is constitutively enhanced in these mutants, but nuclear translocation of mutant FOXO3 is restored with PI3K inhibition. Here we show that on H2O2 exposure, transcription of tumor suppressive miRNAs let-7b and let-7c is regulated by FOXO3 or PRDX1 expression levels and that let-7c is a novel target for FOXO3. Conjointly, inhibition of let-7 microRNAs increases let-7-phenotypes in PRDX1-deficient breast cancer cells. Altogether, these data ascertain the existence of an H2O2-sensitive PRDX1-FOXO3 signaling axis that fine tunes FOXO3 activity toward the transcription of gene targets in response to oxidative stress. Antioxid. Redox Signal. 28, 62â77
NOVA : Rendering Virtual Worlds with Humans for Computer Vision Tasks
Today, the cutting edge of computer vision research greatly depends on the availability of large datasets, which are critical for effectively training and testing new methods. Manually annotating visual data, however, is not only a labor-intensive process but also prone to errors. In this study, we present NOVA, a versatile framework to create realistic-looking 3D rendered worlds containing procedurally generated humans with rich pixel-level ground truth annotations. NOVA can simulate various environmental factors such as weather conditions or different times of day, and bring an exceptionally diverse set of humans to life, each having a distinct body shape, gender and age. To demonstrate NOVA's capabilities, we generate two synthetic datasets for person tracking. The first one includes 108 sequences, each with different levels of difficulty like tracking in crowded scenes or at nighttime and aims for testing the limits of current state-of-the-art trackers. A second dataset of 97 sequences with normal weather conditions is used to show how our synthetic sequences can be utilized to train and boost the performance of deep-learning based trackers. Our results indicate that the synthetic data generated by NOVA represents a good proxy of the real-world and can be exploited for computer vision tasks
Sensitive and quantitative determination of short-chain fatty acids in human serum using liquid chromatography mass spectrometry
Short-chain fatty acids (SCFAs) are increasingly being monitored to elucidate the link between gut health and disease. These metabolites are routinely measured in faeces, but their determination in serum is more challenging due to their low concentrations. A method for the determination of eight SCFAs in serum is described here. High-resolution mass spectrometry and gas chromatography were used to identify the presence of isomeric interferences, which were then overcome through a combination of chromatographic separation and judicious choice of MS fragment ion. The SCFAs were derivatised to form 3-nitrophenylhydrazones before being separated on a reversed-phase column and then detected using liquid chromatography tandem mass spectrometry (LC-QQQ-MS). The LODs and LOQs of SCFAs using this method were in the range 1 to 7 ng mLâ1 and 3 to 19 ng mLâ1, respectively. The recovery of the SCFAs in serum ranged from 94 to 114% over the three concentration ranges tested
Experimental evaluation of the effect of steel and polypropylene fibers and recycled aggregates on the mechanical properties of concrete
In recent years, a new type of fiber-reinforced concrete, consisting of several different types of fibers, known as hybrid fibers reinforced concrete, has attracted the attention of researchers. The aim of this paper for experimental evaluation of the effect of replacement ratio of recycled aggregates with natural aggregates on the mechanical properties of reinforced concrete with hybrid fibers (steel and polypropylene fibers). To this end, reinforced concrete with hybrid fibers with volume fractions of "0.0%" , "0.5%" , and "1%" of steel fibers and "0.0%" and "0.4%" of polypropylene fibers and replacement ratios of "0.0%" , "25%" , and "50%" recycled coarse aggregate of natural coarse aggregates were tested for compressive strength, Brazilian tensile strength, and flexural strength by four-point bending tests. The results show that increasing the replacement ratio of recycled aggregate leads to a decrease in compressive, tensile, and flexural strength. If polypropylene and steel fibers are added to concrete containing recycled aggregates, the compressive, tensile, and flexural strengths of concrete increase, whose steel fibers are more efficient in improving the tensile and flexural strength of concrete than polypropylene fibers. The combination of polypropylene fibers with steel fibers increases energy absorption and increases the flexural toughness of concrete containing recycled aggregates. Moreover, reinforced concrete with hybrid fibers does not disintegrate after breaking, and hybrid fibers play an important role in maintaining the cohesion of concrete
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