549 research outputs found

    Novedades canónicas del año 2005

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    El artículo presenta varios temas y novedades que sucedieron en el año 2005 con respecto a la Iglesia católica. Este año, el acontecimiento más importante y trágico fue el fallecimiento del Papa Juan Pablo II quien llevó más de 26 años en su posición. Durante ese tiempo, Juan Pablo II ha dejado un ejemplo de santidad que sigue influyendo a los millones de católicos del mundo. Con la elección de su sucesor, Benedicto XVI, la Iglesia continuó con su trabajo, particularmente con la introducción de la Instrucción "Dignitas connubii" que discute las causas de nulidad del matrimonio

    Rapid detection of Listeria monocytogenes in food using culture enrichment combined with real-time PCR.

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    A rapid method for the detection of Listeria monocytogenes in foods combining culture enrichment and real-time PCR was compared to the ISO 11290-1 standard method. The culture enrichment component of the rapid method is based on the ISO standard and includes 24h incubation in half-Fraser broth, 4h incubation in Fraser broth followed by DNA extraction and real-time PCR detection of the ssrA gene of L. monocytogenes. An internal amplification control, which is co-amplified with the same primers as the L. monocytogenes DNA, was also included in the assay. The method has a limit of detection of 1-5CFU/25g food sample and can be performed in 2 working days compared to up to 7days for the ISO standard. A variety of food samples from retail outlets and food processing plants (n=175) and controls (n=31) were tested using rapid and conventional methods. The rapid method was 99.44% specific, 96.15% sensitive and 99.03% accurate when compared to the standard method. This method has the potential to be used as an alternative to the standard method for food quality assurance providing rapid detection of L. monocytogenes in food

    Crónica de Derecho Canónico del año 2009

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    Pope Urban II’s Council of Piacenza, Oxford University Press, Oxford-New York 2011 [RESEÑA]

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    obert SOMERVILLE, Pope Urban II’s Council of Piacenza, Oxford University Press, Oxford-New York 2011, VIII + 151 pp
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