980 research outputs found
Microwave Spectroscopy
Contains research objectives, summary of research and reports on two research projects.Joint Services Electronics Programs (U. S. Army, U. S. Navy, and U. S. Air Force) under Contract DAAB07-71-C-030
Antisense Suppression of the Small Chloroplast Protein CP12 in Tobacco Alters Carbon Partitioning and Severely Restricts Growth
Abstract
The thioredoxin-regulated chloroplast protein CP12 forms a multienzyme complex with the Calvin-Benson cycle enzymes phosphoribulokinase (PRK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PRK and GAPDH are inactivated when present in this complex, a process shown in vitro to be dependent upon oxidized CP12. The importance of CP12 in vivo in higher plants, however, has not been investigated. Here, antisense suppression of CP12 in tobacco (Nicotiana tabacum) was observed to impact on NAD-induced PRK and GAPDH complex formation but had little effect on enzyme activity. Additionally, only minor changes in photosynthetic carbon fixation were observed. Despite this, antisense plants displayed changes in growth rates and morphology, including dwarfism and reduced apical dominance. The hypothesis that CP12 is essential to separate oxidative pentose phosphate pathway activity from Calvin-Benson cycle activity, as proposed in cyanobacteria, was tested. No evidence was found to support this role in tobacco. Evidence was seen, however, for a restriction to malate valve capacity, with decreases in NADP-malate dehydrogenase activity (but not protein levels) and pyridine nucleotide content. Antisense repression of CP12 also led to significant changes in carbon partitioning, with increased carbon allocation to the cell wall and the organic acids malate and fumarate and decreased allocation to starch and soluble carbohydrates. Severe decreases were also seen in 2-oxoglutarate content, a key indicator of cellular carbon sufficiency. The data presented here indicate that in tobacco, CP12 has a role in redox-mediated regulation of carbon partitioning from the chloroplast and provides strong in vivo evidence that CP12 is required for normal growth and development in plants.</jats:p
GGD 37: An Extreme Protostellar Outflow
We present the first Spitzer-IRS spectral maps of the Herbig-Haro flow GGD 37 detected in lines of [Ne III], [O IV], [Ar III], and [Ne v]. The detection of extended [O IV] (55 eV) and some extended emission in [Ne v] (97 eV) indicates a shock temperature in excess of 100,000 K, in agreement with X-ray observations, and a shock speed in excess of 200 km s(-1). The presence of an extended photoionization or collisional ionization region indicates that GGD 37 is a highly unusual protostellar outflow.Jet Propulsion Laboratory, under NASA 1407NASA 1257184Jet Propulsion Laboratory (JPL) 960803University of Rochester 31419-5714Astronom
Efficient Implementation and the Product State Representation of Numbers
The relation between the requirement of efficient implementability and the
product state representation of numbers is examined. Numbers are defined to be
any model of the axioms of number theory or arithmetic. Efficient
implementability (EI) means that the basic arithmetic operations are physically
implementable and the space-time and thermodynamic resources needed to carry
out the implementations are polynomial in the range of numbers considered.
Different models of numbers are described to show the independence of both EI
and the product state representation from the axioms. The relation between EI
and the product state representation is examined. It is seen that the condition
of a product state representation does not imply EI. Arguments used to refute
the converse implication, EI implies a product state representation, seem
reasonable; but they are not conclusive. Thus this implication remains an open
question.Comment: Paragraph in page proof for Phys. Rev. A revise
Groundwater : meltwater interaction in a proglacial aquifer
Groundwater plays a significant role in the hydrology of active glacial catchments, with evidence that it may buffer changes in meltwater river flow and partially compensate for glacial loss. However, to date there has been little direct research into the hydrogeology and groundwater dynamics of proglacial aquifers. Here we directly investigate the three dimensional nature of a proglacial sandur (floodplain) aquifer in SE Iceland, using hydrogeological, geophysical, hydrological and stable isotopic techniques, and provide evidence of groundwater-melt water dynamics over three years.
We show that the proglacial sandur forms a thick (at least 50-100 m), high permeability (transmissivity up to 2500 m2/day) aquifer, extending over an area of approximately 6 km2. At least 35 million m3 of groundwater is stored in the aquifer, equivalent to ~23-28% of total annual river flow through the catchment. The volume of mean annual groundwater flow through the aquifer is at least 0.1-1 m3/sec, equivalent to ~10-20% of mean annual river flow. Groundwater across the aquifer is actively recharged from local precipitation and strongly influenced by individual rainfall events and seasonal precipitation. Glacial meltwater influence on groundwater also occurs in a zone extending from 20-500 m away from the meltwater river, for at least 3km down-sandur, and to at least 15 m deep. Within this zone summer recharge from the river to groundwater occurs when meltwater river flows are high, maintaining high summer groundwater levels compared to winter levels; and groundwater temperature and chemistry are strongly influenced by meltwater. Beyond this zone there is no substantial meltwater influence on groundwater.
From ~2 km down-sandur there is extensive groundwater discharge via springs, supporting semi-perennial streams that form distinct local ecosystems, and providing baseflow to the main meltwater river.
This research indicates that predicted continued climate change-related reductions in glacier coverage and increases in precipitation are likely to increase the significance of groundwater storage as a water resource, and of groundwater discharges in maintaining environmental river flows in glacier catchments
The Initial Configuration of Young Stellar Clusters: A K-band Number Counts Analysis of the Surface Density of Stars
We present an analysis of K-band stellar distributions for the young stellar
clusters GGD 12-15, IRAS 20050+2720, and NGC 7129. We find that the two deeply
embedded clusters, GGD 12-15 and IRAS 20050+2720, are not azimuthally symmetric
and show a high degree of structure which traces filamentary structure observed
in 850 micron emission maps. In contrast, the NGC 7129 cluster is circularly
symmetric, less dense, and anti-correlated to 850 micron emission, suggesting
recent gas expulsion and dynamical expansion have occured. We estimate stellar
volume densities from nearest neighbor distances, and discuss the impact of
these densities on the evolution of circumstellar disks and protostellar
envelopes in these regions.Comment: 44 pages, 26 figures, Accepted to ApJ. Changes include extinction
mapping, Monte Carlo field star modeling, and Nyquist sampled azimuthal
stellar distributions. A version with full resolution figures is available at
http://astro.pas.rochester.edu/~rguter/preprints/gutermuth_sd.tar.g
Interpreting ~1 Hz magnetic compressional waves in Mercury's inner magnetosphere in terms of propagating ion‐Bernstein waves
We show that ~1 Hz magnetic compressional waves observed in Mercury's inner magnetosphere could be interpreted as ion‐Bernstein waves in a moderate proton beta ~0.1 plasma. An observation of a proton distribution with a large planetary loss cone is presented, and we show that this type of distribution is highly unstable to the generation of ion‐Bernstein waves with low magnetic compression. Ray tracing shows that as these waves propagate back and forth about the magnetic equator; they cycle between a state of low and high magnetic compression. The group velocity decreases during the high‐compression state leading to a pileup of compressional wave energy, which could explain the observed dominance of the highly compressional waves. This bimodal nature is due to the complexity of the index of refraction surface in a warm plasma whose upper branch has high growth rate with low compression, and its lower branch has low growth/damping rate with strong compression. Two different cycles are found: one where the compression maximum occurs at the magnetic equator and one where the compression maximum straddles the magnetic equator. The later cycle could explain observations where the maximum in compression straddles the equator. Ray tracing shows that this mode is confined within ±12° magnetic latitude which can account for the bulk of the observations. We show that the Doppler shift can account for the difference between the observed and model wave frequency, if the wave vector direction is in opposition to the plasma flow direction. We note that the Wentzel‐Kramers‐Brillouin approximation breaks down during the pileup of compressional energy and that a study involving full wave solutions is required.Key PointsThe ion‐Bernstein (IB) mode is highly unstable to proton loss cones at MercuryThe IB mode can become highly compressional as it propagatesRay tracing of the IB mode predicts compression peaking the off equatorPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/112180/1/jgra51808.pd
Optimizing RuBP regeneration to increase photosynthetic capacity
The regeneration of RuBP is a major factor limiting photosynthesis at sub-saturating light levels. Optimizing this process by overexpressing the enzymes sedoheptulose-1,7-bisphosphatase (SBPase) and fructose-1,6-bisphosphate aldolase (FBP aldolase) is predicted to increase wheat photosynthetic capacity. With combined funding from the
BBSRC and CIMMYT, transgenic lines have been produced to overexpress either SBPase or FBP aldolase in a common UK wheat cultivar and in two CIMMYT lines. Current efforts are characterizing the most promising lines – that is, with considerably more SBPase or FBP aldolase – for further studies of the effects on photosynthetic capacity
A mathematical model for fibro-proliferative wound healing disorders
The normal process of dermal wound healing fails in some cases, due to fibro-proliferative disorders such as keloid and hypertrophic scars. These types of abnormal healing may be regarded as pathologically excessive responses to wounding in terms of fibroblastic cell profiles and their inflammatory growth-factor mediators. Biologically, these conditions are poorly understood and current medical treatments are thus unreliable.
In this paper, the authors apply an existing deterministic mathematical model for fibroplasia and wound contraction in adult mammalian dermis (Olsenet al., J. theor. Biol. 177, 113–128, 1995) to investigate key clinical problems concerning these healing disorders. A caricature model is proposed which retains the fundamental cellular and chemical components of the full model, in order to analyse the spatiotemporal dynamics of the initiation, progression, cessation and regression of fibro-contractive diseases in relation to normal healing. This model accounts for fibroblastic cell migration, proliferation and death and growth-factor diffusion, production by cells and tissue removal/decay.
Explicit results are obtained in terms of the model processes and parameters. The rate of cellular production of the chemical is shown to be critical to the development of a stable pathological state. Further, cessation and/or regression of the disease depend on appropriate spatiotemporally varying forms for this production rate, which can be understood in terms of the bistability of the normal dermal and pathological steady states—a central property of the model, which is evident from stability and bifurcation analyses.
The work predicts novel, biologically realistic and testable pathogenic and control mechanisms, the understanding of which will lead toward more effective strategies for clinical therapy of fibro-proliferative disorders
Maps of Solar Wind Plasma Precipitation onto Mercury’s Surface: A Geographical Perspective
Mercury is the closest planet to the Sun, possesses a weak intrinsic magnetic field, and has only a very tenuous atmosphere (exosphere). These three conditions result in a direct coupling between the plasma emitted from the Sun (namely, the solar wind) and Mercury’s surface. The planet’s magnetic field leads to a nontrivial pattern of plasma precipitation onto the surface that is expected to contribute to the alteration of the regolith over geological timescales. The goal of this work is to study the solar wind plasma precipitation onto the surface of Mercury from a geographical perspective, as opposed to the local time-of-day approach of previous precipitation modeling studies. We employ solar wind precipitation maps for protons and electrons from two fully kinetic numerical simulations of Mercury’s plasma environment. These maps are then integrated over two full Mercury orbits (176 Earth days). We found that the plasma precipitation pattern at the surface is most strongly affected by the upstream solar wind conditions, particularly the interplanetary magnetic field direction, and less by Mercury’s 3:2 spin-orbit resonance. We also found that Mercury’s magnetic field is able to shield the surface from roughly 90% of the incoming solar wind flux. At the surface, protons have a broad energy distribution from below 500 eV to more than 1.5 keV, while electrons are mostly found in the range 0.1-10 keV. These results will help to better constrain space weathering and exosphere source processes at Mercury, as well as interpret observations by the ongoing ESA/JAXA BepiColombo mission
- …