14 research outputs found

    英語授業におけるアクティブ・ラーニングの実質化をめざして

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    This paper deals with the study of the antioxidant activity of diterpene glycoside of stevioside in the absence of stress factors and under the influence of heavy metals. Results showed different response of stevioside to - it reduced catalase activity by 34% as compared to control, but virtually had no effect on the enzymatic activity of an ascorbate peroxidase. Heavy metals at suboptimal concentrations (10 μM) also did not significantly change the activity of the studied enzymes. Growing of plants at a sublethal concentration of pollutants (1 mM) was accompanied by a sharp increase in the activity of ascorbate peroxidase, and, otherwise, decrease in catalase activity. Plants pretreatment with diterpene glycosides ensured reduction of the negative effects of heavy metals on the activity of these enzymes, i.e., ascorbate peroxidase activity was lower and catalase activity was higher. The influence of stevioside also led to 4-fold increase in the content of non-enzymatic antioxidant - proline that may indicate an increasing stress resistance of plants to negative environmental factors

    The antioxidant properties of stevioside under the influence of heavy metals

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    This paper deals with the study of the antioxidant activity of diterpene glycoside of stevioside in the absence of stress factors and under the influence of heavy metals. Results showed different response of stevioside to - it reduced catalase activity by 34% as compared to control, but virtually had no effect on the enzymatic activity of an ascorbate peroxidase. Heavy metals at suboptimal concentrations (10 μM) also did not significantly change the activity of the studied enzymes. Growing of plants at a sublethal concentration of pollutants (1 mM) was accompanied by a sharp increase in the activity of ascorbate peroxidase, and, otherwise, decrease in catalase activity. Plants pretreatment with diterpene glycosides ensured reduction of the negative effects of heavy metals on the activity of these enzymes, i.e., ascorbate peroxidase activity was lower and catalase activity was higher. The influence of stevioside also led to 4-fold increase in the content of non-enzymatic antioxidant - proline that may indicate an increasing stress resistance of plants to negative environmental factors

    Influence of stevioside and heavy metals on physiological and biochemical parameters of winter wheat

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    Diterpene stevioside glycoside ability to increase resistance to heavy metals influence on germinating seedlings of Mironovskaya 808 type winter wheat is studied. It is shown that CdSO4, CuSO4, and ZnSO4 (10 μmol) inhibited the plant growth and caused accumulation of proline amino acid. Preliminary growing on stevioside solution (10-8 mole) for 5 days considerably decreased toxic effect of heavy metals on plant growth, amounts of heavy metals in roots and leaves of germinating seedlings and increased the proline level

    Protector role of stevioside under the influence of heavy metals on the proliferation of sprouts of wheat root cells

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    Diterpenoids is a large family of natural compounds, which in their chemical structure represent the coalescence of carbohydrate cycles, have a different hemometry, and show a polymorphous biological activity. Such compounds include a tetracyclic dipeptide - steviol, which is an agglomerate of glycosides derived from the extract of the herb Stevia Rebaudiana Bertoni. One of the glycosides of this herb is stevioside. This paper deals with the study of the proliferative a ctivity of root cells under the action of the diterpene glycoside - stevioside in the absence of the stress factors and under the action of heavy metals. According to the obtained results, stevioside increases the mitotic activity of the root cell of wheat sprouts by 29%. All the studied heavy metals (Cd, Cu and Zn) inhibited to various extent the proliferation of the root cells at a high concentration (1 mM), while at a low concentration (10 μM) only a cadmium-containing sample showed a reliable difference with the control. Pretreatment with stevioside reduced the negative effect of heavy metals on the mitotic cell activity, which indicates the protective role of stevioside in the influence of pollutants on the plants

    The chemical structure of steviol-glycosides as base of biological activity

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    © 2016,International Journal of Pharmacy and Technology. All rights reserved.This article is dedicated to study of the peculiarities of influence of glycosides extracted from Stevia Rebaudiana plant,namely stevioside,steviolbioside,rebaudioside A and C as well as of commercial mixture of steviol-glycosides “Sweta” on growth as well as on physical and biochemical processes of wheat plants. Compounds with glycoside nature often demonstrate various types of biological activity. The chemical structure of stevia glycosides is similar to the well-known plant growth and development regulating chemicals,i.e. gibberellins and alongside with that these glycosides induce the same physiological reactions in plants as gibberellins do. This fact puts glycosides within the zone of intense interest from the point of view of phytophysiological investigations. Since the difference between the mentioned compounds lies in composition and structure of a carbohydrate branch of a glycoside molecule study of correlation between the structure and the biological influence of such molecules on plants is the question of major priority. The article represents data indicative of direct relation between the chemical structure of a carbohydrate branch of a glycoside molecule and the impact on Triticumaestivum plants induced by it. There was demonstrated ambiguous nature of the studied compounds impact on the physiological and biochemical state of wheat plants

    The antioxidant properties of stevioside under the influence of heavy metals

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    This paper deals with the study of the antioxidant activity of diterpene glycoside of stevioside in the absence of stress factors and under the influence of heavy metals. Results showed different response of stevioside to - it reduced catalase activity by 34% as compared to control, but virtually had no effect on the enzymatic activity of an ascorbate peroxidase. Heavy metals at suboptimal concentrations (10 μM) also did not significantly change the activity of the studied enzymes. Growing of plants at a sublethal concentration of pollutants (1 mM) was accompanied by a sharp increase in the activity of ascorbate peroxidase, and, otherwise, decrease in catalase activity. Plants pretreatment with diterpene glycosides ensured reduction of the negative effects of heavy metals on the activity of these enzymes, i.e., ascorbate peroxidase activity was lower and catalase activity was higher. The influence of stevioside also led to 4-fold increase in the content of non-enzymatic antioxidant - proline that may indicate an increasing stress resistance of plants to negative environmental factors

    The chemical structure of steviol-glycosides as base of biological activity

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    © 2016,International Journal of Pharmacy and Technology. All rights reserved.This article is dedicated to study of the peculiarities of influence of glycosides extracted from Stevia Rebaudiana plant,namely stevioside,steviolbioside,rebaudioside A and C as well as of commercial mixture of steviol-glycosides “Sweta” on growth as well as on physical and biochemical processes of wheat plants. Compounds with glycoside nature often demonstrate various types of biological activity. The chemical structure of stevia glycosides is similar to the well-known plant growth and development regulating chemicals,i.e. gibberellins and alongside with that these glycosides induce the same physiological reactions in plants as gibberellins do. This fact puts glycosides within the zone of intense interest from the point of view of phytophysiological investigations. Since the difference between the mentioned compounds lies in composition and structure of a carbohydrate branch of a glycoside molecule study of correlation between the structure and the biological influence of such molecules on plants is the question of major priority. The article represents data indicative of direct relation between the chemical structure of a carbohydrate branch of a glycoside molecule and the impact on Triticumaestivum plants induced by it. There was demonstrated ambiguous nature of the studied compounds impact on the physiological and biochemical state of wheat plants

    Protector role of stevioside under the influence of heavy metals on the proliferation of sprouts of wheat root cells

    No full text
    Diterpenoids is a large family of natural compounds, which in their chemical structure represent the coalescence of carbohydrate cycles, have a different hemometry, and show a polymorphous biological activity. Such compounds include a tetracyclic dipeptide - steviol, which is an agglomerate of glycosides derived from the extract of the herb Stevia Rebaudiana Bertoni. One of the glycosides of this herb is stevioside. This paper deals with the study of the proliferative a ctivity of root cells under the action of the diterpene glycoside - stevioside in the absence of the stress factors and under the action of heavy metals. According to the obtained results, stevioside increases the mitotic activity of the root cell of wheat sprouts by 29%. All the studied heavy metals (Cd, Cu and Zn) inhibited to various extent the proliferation of the root cells at a high concentration (1 mM), while at a low concentration (10 μM) only a cadmium-containing sample showed a reliable difference with the control. Pretreatment with stevioside reduced the negative effect of heavy metals on the mitotic cell activity, which indicates the protective role of stevioside in the influence of pollutants on the plants

    The chemical structure of steviol-glycosides as base of biological activity

    Get PDF
    © 2016,International Journal of Pharmacy and Technology. All rights reserved.This article is dedicated to study of the peculiarities of influence of glycosides extracted from Stevia Rebaudiana plant,namely stevioside,steviolbioside,rebaudioside A and C as well as of commercial mixture of steviol-glycosides “Sweta” on growth as well as on physical and biochemical processes of wheat plants. Compounds with glycoside nature often demonstrate various types of biological activity. The chemical structure of stevia glycosides is similar to the well-known plant growth and development regulating chemicals,i.e. gibberellins and alongside with that these glycosides induce the same physiological reactions in plants as gibberellins do. This fact puts glycosides within the zone of intense interest from the point of view of phytophysiological investigations. Since the difference between the mentioned compounds lies in composition and structure of a carbohydrate branch of a glycoside molecule study of correlation between the structure and the biological influence of such molecules on plants is the question of major priority. The article represents data indicative of direct relation between the chemical structure of a carbohydrate branch of a glycoside molecule and the impact on Triticumaestivum plants induced by it. There was demonstrated ambiguous nature of the studied compounds impact on the physiological and biochemical state of wheat plants
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