Experiences in teaching Hydrogen Technologies in the framework of the International Campus of Excellence Andalucia TECH

The increasing importance of hydrogen technologies is demanding prepared professionals in the numerous topics related to this energy vector. Apart from some basic and advanced courses given by actual experts in these topics, universities must play an important role in the general formation of future professionals. In this way, the International Campus of Excellence (ICE) Andalucía TECH has created several degrees taught jointly by its participating universities of Seville (US) and Málaga (UMA). Among those degrees, Energy Engineering is the best suited to the field of this conference because this degree provide future engineers with specialized training in energy generation, transformation and management. In relation to hydrogen technologies this degree includes a cross sectional optional unit named Hydrogen Based Systems (4.5 ECTS/112.5 h), which is placed in the last term of the fourth year and sharing space with work placement and mobility courses. This paper will present some author’s experiences as teachers of that unit during its short history of only three years. In spite of being a joint degree, which is coordinated between both universities (US and UMA) trying to give similar contents, it is interesting to highlight the differential experiences coming from the teachers of both universities in relation to the teaching methodologies and academic results.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Desalination effluents and the establishment of the non-indigenous skeleton shrimp Paracaprella pusilla Mayer, 1890 in the south-eastern Mediterranean

A decade long monitoring programme has revealed a flourishing population of the non-indigenous skeleton shrimp Paracaprella pusilla in the vicinity of outfalls of desalination plants off the Mediterranean coast of Israel. The first specimens were collected in 2010, thus predating all previously published records of this species in the Mediterranean Sea. A decade-long disturbance regime related to the construction and operation of the plants may have had a critical role in driving the population growth

Distribución vertical de la macrofauna en sedimentos contaminados del interior del puerto de Ceuta

Se ha estudiado la distribución vertical de la macrofauna y su relación con las variables fisicoquímicas de los sedimentos contaminados del puerto de Ceuta. La obtención de las muestras se llevó a cabo mediante buceo con escafandra autónoma y empleando instrumentos de muestreo tipo core (10 cm × 17 cm × 35 cm), diferenciándose las siguientes profundidades en la columna de sedimento: 0-2 cm, 2-5 cm, 5-10 cm y más de 10 cm. Los crustáceos Pariambus typicus Kröyer, 1844; Apseudes latreilli Milne-Edwards, 1820; Corophium runcicorne Della Valle, 1893; C. sextonae Crawford, 1937; el molusco Parvicardium exiguum (Gmelin, 1791) y los poliquetos Pseudomalacoceros tridentata (Southern, 1914) y Exogone verrugera Cleparede, 1868 se localizaron en los dos primeros centímetros de sedimento, mientras que el molusco Loripes lacteus (L., 1758) y los poliquetos Platynereis dumerilii Audouin y Milne-Edwards, 1833; Cirratulus cirratus (Muller, 1776) y Cirriformia tentaculata (Montagu, 1808) dominaron en los niveles más profundos, observándose el incremento en el tamaño de los individuos con el aumento de la profundidad. Los análisis multivariantes reflejaron que el porcentaje de pelitas, la materia orgánica total y, especialmente, la relación lípidos malténicos/lípidos asfalténicos, fueron los principales factores condicionantes de la distribución vertical de las especies en el sedimento.Vertical distribution of the macrofauna in polluted sediments from the harbour of Ceuta Vertical distribution of the macrofauna and its relationship with physico-chemical parameters were studied in polluted sediments from the harbour of Ceuta. The samples were collected with cores (10 cm × 17 cm × 35 cm) by scuba divers. The following depths in the sediment column were included in the study: 0-2 cm, 2- 5 cm, 5-10 cm and more than 10 cm. The crustaceans Pariambus typicus Kröyer, 1844; Apseudes latreilli Milne-Edwards, 1820; Corophium runcicorne Della Valle, 1893; C. sextonae Crawford, 1937; the mollusc Parvicardium exiguum (Gmelin, 1791), and the polychaetes Pseudomalacoceros tridentata (Southern, 1914) and Exogone verrugera Cleparede, 1868 were found in the first 2 cm of the sediment, whereas the mollusc Loripes lacteus (L., 1758) and the polychaetes Platynereis dumerilii Audouin & Milne Edwards, 1833; Cirratulus cirratus (Muller, 1776) and Cirriformia tentaculata (Montagu, 1808) were dominant in deeper strata, and size increased with depth. A multivariate analysis showed that the percentage of silt and clays, the total organic matter, and especially, the maltenes/asphaltenes ratio, were the main factors affecting species’ vertical distribution

Redescription of Caprella hirsuta Mayec 1890 (Crustacea, Amphipoda, Caprellidea) from the Strait of Gibraltar

Caprella hirsuta Mayer, 1890 is redescribed based on specimens collected from the Strait of Gibraltar (Southern Spain-Northern Africa) during a study of the amphipod fauna from these coasts. Careful examination of these caprellids revealed differences with the previous descriptions, mainly the structure of gnathopod 2, pereopods and abdomen. Key words: Crustacea, Amphipoda, Caprellidea, Caprella hirsuta, Strait of Gibraltar, Redescription.Caprella hirsuta Mayer, 1890 is redescribed based on specimens collected from the Strait of Gibraltar (Southern Spain-Northern Africa) during a study of the amphipod fauna from these coasts. Careful examination of these caprellids revealed differences with the previous descriptions, mainly the structure of gnathopod 2, pereopods and abdomen. Key words: Crustacea, Amphipoda, Caprellidea, Caprella hirsuta, Strait of Gibraltar, Redescription.Caprella hirsuta Mayer, 1890 is redescribed based on specimens collected from the Strait of Gibraltar (Southern Spain-Northern Africa) during a study of the amphipod fauna from these coasts. Careful examination of these caprellids revealed differences with the previous descriptions, mainly the structure of gnathopod 2, pereopods and abdomen. Key words: Crustacea, Amphipoda, Caprellidea, Caprella hirsuta, Strait of Gibraltar, Redescription

Seasonal variations of the vagile fauna associated with Ectopleura crocea (Cnidaria, Hydrozoa) in El Rompido marina (Huelva)

El desarrollo urbano en las zonas costeras se ha visto incrementado notablemente en los últimos años, resultando en un mayor número de estructuras artificiales en los ambientes marinos. Diversos organismos han sido capaces de colonizar las superficies de estos nuevos hábitats, incluyendo al hidrozoo Ectopleura crocea (Agassiz, 1862). Se estudió la epifauna asociada a esta especie a lo largo de un año en Puerto Marina, El Rompido, Huelva, con el objetivo de describir su composición y fluctuaciones temporales. En total, se encontraron 22 especies, mayoritariamente crustáceos anfípodos, siendo los más abundantes Stenothoe tergestina (Nebeski, 1881) y Apocorophium acutum (Chevreux, 1908). No se encontraron diferencias significativas a lo largo del ciclo anual en los valores de abundancia y diversidad, si bien se detectaron cambios en la composición faunística. En general, la diversidad de especies encontrada en Ectopleura crocea fue menor que la citada en otros estudios en hábitats naturales. Se sugiere que ello puede ser debido a características intrínsecas del hábitat artificial, tales como una menor heterogeneidad de sus superficies o a un mayor estrés ambiental. Por ello, para contrastar la consistencia a lo largo del tiempo y la causa de tales patrones, sería necesario estudios futuros que abarcasen un mayor rango temporal.Urban development in coastal areas has increased considerably in recent years, which has resulted in a larger number of artificial structures in coastal marine environments. However, various organisms have been able to colonize the surfaces of these new habitats, including the hydrozoan Ectopleura crocea (Agassiz, 1862). We studied the epifauna associated with this species over a year in Puerto Marina, El Rompido, Huelva, with the objective of describing its composition and temporal fluctuations. In total, 22 species were found, mostly amphipod crustaceans, the most abundant of these being Stenothoe tergestina (Nebeski, 1881) and Apocorophium acutum (Chevreux, 1908). No significant differences were found between the abundance and diversity of epifauna throughout the year, although changes in epifauna composition were detected over time. In general, the diversity found on Ectopleura crocea in the present study was lower than that reported by other studies in natural habitats. It is suggested that this could be due to intrinsic characteristics of the artificial habitat, such as lower heterogeneity of its surfaces or greater environmental stress. To contrast consistency over time and the causes of these patterns future studies carried out over a larger time period will be necessar

Cambios ontogenéticos e intraespecíficos observados en la rádula de Polycera aurantiomarginata García and Bobo, 1984 (Gastropoda Opisthobranchia)

Polycera aurantiomarginata García and Bobo 1984 has a stable population in the intertidal area of El Portil beach (Huelva, SW Spain). This fact allowed specimens of different sizes to be collected from March 2001 to December 2003. In this paper, the ontogenetic variations of the radula of P. aurantiomarginata are studied. The radulae of 141 specimens were examined, 138 from El Portil and 3 from La Herradura (Granada, SE Spain). Specimens of 1.5-2 mm in length lack the typical radula described for P. aurantiomarginata. They have the so called pre-radula whose teeth are different in size and shape from the typical radula of the adults. In the specimens of 3 and 4 mm the pre-radula coexists with the characteristic radula, which is the single structure present in the specimens larger than 4 mm. The following features of the radula are included in this study: radular length, number of teeth rows and length of the outer lateral teeth. According to the three measured variables, the affinities among specimens without a pre-radula were established through cluster analysis, which defined three different groups (4-10 mm, 11-22 mm and 23-48 mm). Correlations between specimen length and radula length, number of rows and mean length of outer lateral teeth were significant. Feeding strategies could be related to the different morphology of the radula established by the Cluster analysis.Polycera aurantiomarginata García y Bobo, 1984 muestra una población estable y en la localidad de El Portil (Huelva, SW de España), lo que ha permitido la recolección, desde marzo de 2001 hasta diciembre de 2003, de un alto número de ejemplares de todos los tamaños. En el presente estudio se ha extraído la rádula de 141 animales, 138 recogidos en la zona intermareal de El Portil y 3 en La Herradura (Granada). Los tamaños de los animales han oscilado entre 1.5 mm y 48 mm. Se ha podido observar en los individuos de entre 1.5 y 2 mm la existencia de una pre-rádula cuyos dientes son morfológicamente diferentes a los de la rádula de los individuos mayores; sin embargo, en los ejemplares de 3 y 4 mm esta pre-rádula coexiste con la rádula típica, siendo esta estructura la única presente en individuos de longitud igual o mayor a 4 mm. A cada una de las rádulas extraídas, tanto con pre-rádula o sin ella, se le ha medido la longitud total de la cinta, la longitud del diente lateral externo y el número de filas de dientes. Considerando los tres parámetros medidos, las afinidades entre los ejemplares sin pre-rádula se establecieron a partir de análisis de Cluster, que definieron tres grupos distintos (4-10 mm, 11-22 mm y 23-48 mm). Las correlaciones existentes entre la longitud de los individuos y la longitud de la rádula, el número de filas y la longitud media de los dientes fueron significativas. Las diferencias morfológicas reconocidas en los grupos considerados podrían estar relacionadas con distintas estrategias alimentarias.

Characterization of a proximal Sp1 response element in the mouse Dlk2 gene promoter

<p>Abstract</p> <p>Background</p> <p>DLK2 is an EGF-like membrane protein, closely related to DLK1, which is involved in adipogenesis. Both proteins interact with the NOTCH1 receptor and are able to modulate its activation. The expression of the gene <it>Dlk2 </it>is coordinated with that of <it>Dlk1 </it>in several tissues and cell lines. Unlike <it>Dlk1</it>, the mouse <it>Dlk2 </it>gene and its locus at chromosome 17 are not fully characterized.</p> <p>Results</p> <p>The goal of this work was the characterization of <it>Dlk2 </it>mRNA, as well as the analysis of the mechanisms that control its basal transcription. First, we analyzed the <it>Dlk2 </it>transcripts expressed by several mouse cells lines and tissues, and mapped the transcription start site by 5' Rapid Amplification of cDNA Ends. <it>In silico </it>analysis revealed that <it>Dlk2 </it>possesses a TATA-less promoter containing minimal promoter elements associated with a CpG island, and sequences for Inr and DPE elements. Besides, it possesses six GC-boxes, considered as consensus sites for the transcription factor Sp1. Indeed, we report that Sp1 directly binds to the <it>Dlk2 </it>promoter, activates its transcription, and regulates its level of expression.</p> <p>Conclusions</p> <p>Our results provide the first characterization of <it>Dlk2 </it>transcripts, map the location of the <it>Dlk2 </it>core promoter, and show the role of Sp1 as a key regulator of <it>Dlk2 </it>transcription, providing new insights into the molecular mechanisms that contribute to the expression of the <it>Dlk2 </it>gene.</p

Chromosomal mapping of rRNA genes, core histone genes and telomeric sequences in Brachidontes puniceus and Brachidontes rodriguezi (Bivalvia, Mytilidae)

<p>Abstract</p> <p>Background</p> <p>Chromosome rearrangements are an important part of the speciation process in many taxa. The study of chromosome evolution in bivalves is hampered by the absence of clear chromosomal banding patterns and the similarity in both chromosome size and morphology. For this reason, obtaining good chromosome markers is essential for reliable karyotypic comparisons. To begin this task, the chromosomes of the mussels <it>Brachidontes puniceus </it>and <it>B. rodriguezi </it>were studied by means of fluorochrome staining and fluorescent <it>in situ </it>hybridization (FISH).</p> <p>Results</p> <p><it>Brachidontes puniceus </it>and <it>B. rodriguezi </it>both have 2n = 32 chromosomes but differing karyotype composition. Vertebrate-type telomeric sequences appear at both ends of every single chromosome. <it>B. puniceus </it>presents a single terminal major rRNA gene cluster on a chromosome pair while <it>B. rodriguezi </it>shows two. Both mussels present two 5S rDNA and two core histone gene clusters intercalary located on the long arms of two chromosome pairs. Double and triple-FISH experiments demonstrated that one of the 5S rDNA and one of the major rDNA clusters appear on the same chromosome pair in <it>B. rodriguezi </it>but not in <it>B. puniceus</it>. On the other hand, the second 5S rDNA cluster is located in one of the chromosome pairs also bearing one of the core histone gene clusters in the two mussel species.</p> <p>Conclusion</p> <p>Knowledge of the chromosomal distribution of these sequences in the two species of <it>Brachidontes </it>is a first step in the understanding of the role of chromosome changes on bivalve evolution.</p