A major T cell antigen of Mycobacterium leprae is a 10-kD heat-shock cognate protein.

Several mycobacterial antigens, identified by monoclonal antibodies and patient sera, have been found to be homologous to stress or heat-shock proteins (hsp) defined in Escherichia coli and yeast. A major antigen recognized by most Mycobacterium leprae-reactive human T cell lines and cell wall-reactive T cell clones is a 10-kD protein that has now been cloned and sequenced. The predicted amino acid sequence of this protein is 44% homologous to the hsp 10 (GroES) of E. coli. The purified native and recombinant 10-kD protein was found to be a stronger stimulator of peripheral blood T cell proliferation than other native and recombinant M. leprae proteins tested. The degree of reactivity paralleled the response to intact M. leprae throughout the spectrum of leprosy. Limiting-dilution analysis of peripheral blood lymphocytes from a patient contact and a tuberculoid patient indicated that approximately one third of M. leprae-reactive T cell precursors responded to the 10-kD antigen. T cell lines derived from lepromin skin tests were strongly responsive to the 10-kD protein. T cell clones reactive to both the purified native and recombinant 10-kD antigens recognized M. leprae-specific epitopes as well as epitopes crossreactive with the cognate antigen of M. tuberculosis. Further, the purified hsp 10 elicited strong delayed-type hypersensitivity reactions in guinea pigs sensitized to M. leprae. The strong T cell responses against the M. leprae 10-kD protein suggest a role for this heat-shock cognate protein in the protective/resistant responses to infection

EC98-761 Farm*A*Syst Nebraska’s System for Assessing Water Contamination Worksheet 10: Livestock Yards Management

Why should I be concerned? Livestock yards, such as barnyards, holding areas and feedlots, are areas of concentrated livestock wastes and a potential source of nutrient, solids, and bacteria contamination of surface and groundwater. Yards that are abandoned permanently or are inactive temporarily also represent a potential risk

EC98-772 Farm*A*Syst Nebraska’s System for Assessing Water Contamination Worksheet 8: Household Wastewater Treatment

Why should I be concerned? Virtually all farms and rural housing use a septic system or similar on-site wastewater treatment system. While these systems are generally economical and safe, household wastewater can contain contaminants that degrade water quality for uses such as drinking, pet and stock watering, food preparation, and cleaning. Potential contaminants in household wastewater include disease-causing bacteria, infectious viruses, household chemicals, and excess nutrients, such as nitrate

EC98-795 Farm*A*Syst Nebraska’s System for Assessing Water Contamination Fact Sheet 17: Improving Stormwater Management at Residential Sites

Stormwater is water from irrigation, rain or melting snow that does not soak into the ground. It flows from rooftops, over paved areas and bare soil, and across sloped lawns. As it flows, this runoff can collect and transport soil, pet waste, livestock manure, salt, pesticides, fertilizer, oil and grease, leaves, litter and other potential pollutants. A heavy rainstorm isn’t needed to send pollutants rushing toward streams, wetlands and lakes. A garden hose alone can supply enough water

EC98-749 Farm*A*Syst Nebraska’s System for Assessing Water Contamination Risk Fact Sheet 12: Improving Silage Storage

Silage is an important feed for livestock-based agriculture. When properly harvested and stored, silage poses little or no pollution threat, but improper handling can lead to a significant flow of silage juices (or leachate) from the silo. Leachate is an organic liquid that results from pressure in the silo or from extra water entering the silo. It is usually a problem only when silage is fresh, or just after storage. This loss of leachate represents a loss of nutrient value from the silage

EC98-767 Farm*A*Syst Nebraska’s System for Assessing Water Contamination Fact Sheet 4: Improving Pesticide Storage and Handling

We’ll look at five areas of pesticide management on your farm: 1. pesticide storage practices 2. mixing and loading practices 3. spill cleanup 4. container disposal practices 5. other management practices When handling pesticides, wear proper protective clothing and equipment at all times. See the pesticide label for details

Relationship between Massachusetts Youth Screening Instrument-second version and psychiatric disorders in youths in welfare and juvenile justice institutions in Switzerland

BACKGROUND: There is growing evidence that it is important to have well-standardized procedures for identifying the mental health needs of youths in welfare and juvenile justice institutions. One of the most widely used tools for mental health screening in the juvenile justice system is the Massachusetts Youth Screening Instrument-second version (MAYSI-2). To contribute to the body of research examining the utility of the MAYSI-2 as a mental health screening tool; the first objective of the current study was to examine the relationship between the MAYSI-2 and the Schedule for Affective Disorders and Schizophrenia for School-Age Children, Present and Lifetime version (K-SADS-PL) in a sample of Swiss youths in welfare and juvenile justice institutions using a cross-sectional design. Secondly, as the sample was drawn from the French-, German- and Italian-speaking parts of Switzerland, the three languages were represented in the total sample and consequently differences between the language regions were analyzed as well. The third objective was to examine gender differences in this relationship. METHODS: Participants were 297 boys and 149 girls (mean age = 16.2, SD = 2.5) recruited from 64 youth welfare and juvenile justice institutions in Switzerland. The MAYSI-2 was used to screen for mental health or behavioral problems that could require further evaluation. Psychiatric classification was based on the Schedule for Affective Disorders and Schizophrenia for School-Age Children, Present and Lifetime version (K-SADS-PL). Binomial logistic regression analysis was used to predict (cluster of) psychiatric disorders from MAYSI-2 scales. RESULTS: The regression analyses revealed that the MAYSI-2 scales generally related well to their corresponding homotypic (cluster of) psychiatric disorders. For example, the alcohol/drug use scale identified the presence of any substance use disorder and the suicide ideation scale identified youths reporting suicide ideation or suicide attempts. Several MAYSI-2 scales were also related to heterotypic (cluster of) psychiatric disorders. For example, the MAYSI-2 scale alcohol/drug use, was positively related to any disruptive disorder. Furthermore, the results revealed gender differences in the relationship between the MAYSI-2 and K-SADS-PL (e.g., in the boys\u27 subsample no MAYSI-2 scale was significantly related to any affective disorder; whereas, in the girls\u27 subsample the MAYSI-2 scales depressed-anxious and somatic complaints were significantly related to any affective disorder). CONCLUSIONS: Overall, The MAYSI-2 seems to serve well as a first-stage screen to identify service needs for youths in welfare and juvenile justice institutions in Switzerland. Its effectiveness to identify the presence of (cluster of) psychiatric disorders differs between genders